Labshake search
Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
-
bioRxiv - Cell Biology 2020Quote: ... Nucleoporin-specific antibody was used for primary staining (over-night incubation at 4°C) and and AlexaFluor-conjugated secondary antibodies (Invitrogen; 2 hours at RT). Microscopy was performed as previously described 16 ...
-
bioRxiv - Microbiology 2021Quote: ... Healthy cell culture of the primmorphs (2–4 mm in diameter) of green color were transferred to the 24-well plates (Nalge Nunc International, Rochester, NY, USA), 1 pieces per well ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 2 h at 4℃ with Alexa fluor 488-conjugated secondary antibody (1:200, Invitrogen, #A21206, Carlsbad, CA, USA) or DAPI (1:30000 ...
-
bioRxiv - Neuroscience 2022Quote: ... acutely dissociated DRG neurons from 4 mice were first incubated in at 37 °C for 30 minutes in 4 mm Fura-2-acetoxymethyl ester and 0.2% Pluronic® F127 (Thermo Fisher Scientific, Waltham, MA), washed three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... Next day the cells were washed with fresh media and incubated at 37°C for 45 min in culture media containing 4 μM Fura-2/acetoxymethyl ester (Molecular Probes, Eugene, OR, USA). Then the cells were washed and kept in HEPES-buffered saline solution (140 mM NaCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... CKY2038 was grown to mid-log (1-2×107 cells/mL) in SC liquid media and added to the ConA treated perfusion chamber gasket (ThermoFisher, 4 chamber: 19 × 6mm) for treatment at indicated conditions (SC+1% DMSO ...
-
bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific, Basel, Switzerland). The beads were washes 5 times with lysis buffer and proteins were eluted in TEV buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... A baculovirus stock carrying the gene for DNMT1 was used to infect 4–12 l of Sf9 cells at a density of 2 million cells/ml in Sf-900™ III SFM (Invitrogen cat # 12658–027). Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044 ...
-
bioRxiv - Biochemistry 2022Quote: ... the cleared lysates were incubated for 2 h at 4°C with prewashed anti-c-myc MagBeads (Pierce Thermo Fisher Scientific, product number 88843). After five washes with lysis buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
-
bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
-
bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... 2-3 ml RBC Lysing Buffer (Invitrogen) was added to the pellet containing splenocytes and incubated at room temperature for 5-7 min ...
-
bioRxiv - Microbiology 2020Quote: ... and IFNλ−2/3 (Thermo Scientific Mm04204156_gH) and results were normalized to GAPDH (Mm.PT.39a.1 ...
-
bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
-
bioRxiv - Developmental Biology 2021Quote: ... cells were cultured for 4 days with 1 µM 4-OHT in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with GlutaMax (Thermo Fisher Scientific), non-essential amino acids (NEAA ...
-
bioRxiv - Neuroscience 2020Quote: ... Third instar larvae were dissected with cold HL3 and immediately fixed with PFA (4%) and incubated overnight at 4 C with primary antibodies (rabbit anti-Dlg, 1:1000; anti-Brp 1:100, Life Technologies). Alexa-conjugated secondary antibodies were used for secondary staining (Jackson Laboratories 1:500) ...
-
bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were ultracentrifuged for 4 hours at 50.000 rpm at 4°C in a Sorvall MX 120/150 Plus MicroUltracentrifuge (Thermo Fisher Scientific) in S55A2 rotor ...
-
bioRxiv - Immunology 2020Quote: ... mice were anesthetized using isofluorane and perfused transcardially with 4 mL PBS followed by 4 mL RNAlater™ (Invitrogen; CAT # AM7020) using a peristaltic pump at a flow rate of 0.8mL/min ...
-
bioRxiv - Biophysics 2021Quote: ... The grids were blotted at blotting force 20 for 4 s at 4°C and 100% humidity and plunged into liquid ethane using a Vitrobot (Thermo Scientific).
-
bioRxiv - Synthetic Biology 2020Quote: ... Cellular response in each well was measured via fluo-4 intracellular calcium dye using the fluo-4 NW calcium assay kit (Molecular Probes) according to manufacturer’s directions ...
-
bioRxiv - Neuroscience 2023Quote: ... MBL) for 4 days at 4°C and with Alexa-Fluor (AF) 488 goat anti-rabbit IgG (R37116, Thermo Fisher Scientific) overnight at RT followed by DAPI staining (1:10,000 ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 22,000 × g at 4°C for 20 minutes and poured over 4 mL of HisPur Cobolt resin (89964, Thermo Fisher Scientific). The slurry was rotated at 4°C for 1 hour and spun down at 700 × g for 2 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Genetics 2023Quote: ... Extracts were incubated with rotation with rabbit polyclonal anti-Piwi antibodies (4 µg per sample) at 4°C for 4h followed by overnight incubation with DynabeadsTM Protein A (50 µl, Invitrogen, 10002D) at 4°C with rotation ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: A fraction of the liver from each mouse was preserved in 4% formalin (Cat# SF100-4, Thermo Fisher Scientific, Waltham, MA) followed by 70% ethanol for histology ...
-
bioRxiv - Immunology 2023Quote: ... blotted for 4 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher). All grids were screened using a ThermoFisher Glacios microscope (OSU Center for Electron Microscopy and Analysis).
-
bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, NW04122BOX) and transferred onto a PVDF membrane (Immobilon-FL ...
-
bioRxiv - Neuroscience 2022Quote: ... Three successive 5-hour paraffin wax embedding stages were performed followed by cooling and sectioning of tissue on a Leica microtome into 4 × 4 μm thick serial sections that were collected on Superfrost (ThermoFisher Scientific) microscope slides ...
-
bioRxiv - Molecular Biology 2022Quote: ... the mix was cooled down to 4°C and supplemented with 1 μl of a solution containing 4 U Terminal Transferase (Thermo Scientific), 0.1 μl 10X NEB 4 buffer (NEB) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The mixture of SUMO protease cleavage products was dialyzed overnight (4 ºC, 4 L, 20 mM Tris-HCl, 150 mM NaCl, Slide-A-Lyzer→ cassette, 10 kDa, Thermo Scientific). The hexa-histidine tagged SUMO protein was separated from non-tagged authentic Mpro present in the dialysate by IMAC and collecting Mpro in the flow through ...
-
bioRxiv - Neuroscience 2024Quote: ... larvae and dissected adult brains were fixed overnight in paraformaldehyde (PFA; 4% in 1x phosphate-buffered saline) at 4°C then dehydrated overnight in 100% methanol (A4124, Fisher Scientific) at -20°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Separation of anionic metabolites were performed using a Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific). The injection volume was 0.4 µL ...
-
bioRxiv - Cell Biology 2024Quote: ... Click-iT reaction cocktail (100 Mm Tris-buffered saline, 4 mM CuSO4, 100 mM ascorbic acid and 4 µM Alexa FluorTM488 azide (Invitrogen #A10266) was used for detecting EU ...
-
bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 g for 3 min and supernatant was run on 4–20% Bio-Rad 4–20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... -4 blot force for 4 seconds and plunged frozen in liquid ethane using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected on a Titan Krios operating at 300 keV and equipped with a Gatan K3 direct detector and a 20 eV slit width energy filter ...
-
bioRxiv - Biochemistry 2022Quote: Protein samples in 1X LDS sample buffer containing 4% β-mercaptoethanol (BME) were separated by size on 4-12% Novex NuPage Bis-Tris SPS-PAGE (Invitrogen) gels (2 h ...
-
bioRxiv - Genetics 2022Quote: ... Larval brains were fixed in 25 ml of acetic acid: 4% formaldehyde in PBS (45%:55%) for 4 min on a clean Superfrost plus slide (Fisher Scientific) and the sample was manually squashed via thumb/stamp over coverslip ...
-
bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
-
bioRxiv - Cell Biology 2023Quote: ... Extracts were clarified by centrifugation twice for 15 minutes at 16,000 rcf at 4°C and 100 μl embryo extract was rotated at 4°C overnight with 30 μl magnetic ProteinA dynabeads (Life Technologies) coupled with anti-MBP antibodies (gift from Jordan Raff ...
-
bioRxiv - Immunology 2023Quote: ... Drosophila S2 cells were mixed with Ramos cells or B1-8hi B cells at a ratio of 1:4 (4×105 B cells and 1×105 S2 cells) followed by total RNA extraction with TRIzol reagent (Thermo Fisher), DNaseI digestion ...
-
THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were washed with ice-cold PBS, harvested, and incubated (30 min, 4 °C) with rabbit anti-AF488 IgG (4 µg/mL, Thermo Fisher). AF488 fluorescence was quantified by flow cytometry (BD FACSCanto II flow cytometer ...
-
bioRxiv - Plant Biology 2024Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antibody or IgG (as control) was added and incubated for 4 hrs at 4°C with Protein G Dynabeads (Life Technologies) for antigen coupling ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissue specimens were fixed with 4% PBS-buffered (pH 7.4) paraformaldehyde for 12 h at 4°C before immersion in 30% sucrose and freezing in OCT (LAMB/OCT, Thermo Scientific) in cryomolds ...
-
bioRxiv - Neuroscience 2024Quote: Perfused brains were post-fixed overnight at 4°C in 4% PFA then cryoprotected in 30% (w/v) sucrose (Fisher Scientific) solution in PBS for at least 48 hr prior to processing ...
-
bioRxiv - Neuroscience 2024Quote: ... Brain slices were then fixed for 30 minutes in 4% PFA in PBS at 4 and labeled with streptavidin conjugated with Alexa Fluor 568 (ThermoFisher S11226) for three hours at room temperature ...