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Citations for Thermo Fisher :
3151 - 3200 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Stained platelets were then washed in blocking buffer and mounted on poly-L-ornithine-coated glass slides using ProLongTM Glass Antifade mountant (Invitrogen), covered with coverslips and stored at 4 °C until imaging by multiphoton microscopy.
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed 4X in PBS with .5% Triton X-100 (PBS-T) and then placed in blocking solution (PBS-T with 5% normal goat serum (NGS; Invitrogen)) for 90 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed three times with 1X PBS for 5 minutes each and blocked for 30 minutes at RT with Blocking Buffer [0.4% acetylated BSA (Ambion, AM2614) in 4X SSC] in a humidified chamber ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions with primary antibodies were performed in blocking buffer containing rabbit anti-GFP (1:1000, Invitrogen, Carlsbad, CA, USA, A11122) and/or goat anti-c-Fos (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Triton X-100 for 5 min and incubated with a blocking buffer containing PBS and 5% normal goat serum (31873; Invitrogen) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in Odyssey Blocking Buffer containing HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) or anti-mouse IgG (H+L ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed three times with 0.01% Triton X-100 in PBS for 5 minutes each and then incubated in blocking solution containing corresponding secondary antibodies labeled with Alexa fluorophores (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... All stains were carried out in media containing anti-CD16/32 blocking antibody (ThermoFisher, clone 93, catalog # 14-0161-86). All flow cytometry was acquired on an LSRFortessa FACS analyzer ...
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bioRxiv - Genetics 2019Quote: ... was applied at a final concentration of 1:300 in blocking buffer for 1 hr and Hoechst 33342 (ThermoFisher #H3570) applied for 1-5 min at a concentration of 1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000; Invitrogen 22C5D8 Pgk1 at 1:6,000 ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was in-cubated for 1 h at room temperature with Pierce Protein-Free Blocking Buffer (Thermo Fisher Scientific) and again washed with PBS three times ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were washed six times with blocking solution and then incubated for two hours with Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen, USA ...
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bioRxiv - Biochemistry 2020Quote: ... After transfer and blocking of polyvinylidene fluoride (PVDF) membranes (Immobilon) in 5% BSA TBS Tween-20 buffer (Thermo Fisher Scientific), primary antibody directed against FADS2 (PA5-87765 ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were washed with PBS and incubated overnight at 4° C with blocking buffer (Thermo Scientific, Waltham, MA, cat. #37572) to eliminate nonspecific sites ...
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bioRxiv - Bioengineering 2020Quote: ... After incubation with excess streptavidin and biotin to block endogenous biotin (Endogenous Biotin Blocking Kit; Thermo Fisher Scientific, Waltham, MA), 5 µM of biotinylated HCDR3-construct was incubated on tissue overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were blocked in Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (Mouse anti-V5 antibody, Invitrogen R960-25 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 min followed by incubation with secondary antibodies diluted 1:500 in blocking buffer: goat anti-Chicken Alexa Fluor®546 (RRID: AB_2534097, ThermoFisher) and Goat anti-Rabbit Alexa Fluor®488 (RRID:AB_2534096 ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217 ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies diluted 1:1000 in blocking buffer (donkey anti-mouse Alexa 488, donkey anti-rabbit Alexa Fluor 555 or 647, Invitrogen) were applied for 4 hours at room temperature with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated in blocking solution containing secondary antibodies for 24 hr (streptavidin AlexaFluor conjugate, Invitrogen; 1:250 goat anti-mouse AlexaFluor conjugate, Invitrogen). Following staining ...
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bioRxiv - Cell Biology 2021Quote: ... secondary labeling was performed by addition of the following antibodies diluted in blocking/ permeabilization buffer for 30 minutes at room temperature: Alexa Fluor 647 or 488 donkey anti-mouse (Invitrogen), Alexa Fluor 647 or 488 donkey anti-rabbit (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was crosslinked using a UV stratalinker and blocked at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Ambion) for 2 hours while rotating ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:100 in blocking buffer and the secondary antibody (Anti-mouse IgG conjugated with Alexa 635, Goat IgG - Invitrogen) diluted 1:300 in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... The wells were then washed 5 times with phosphate-buffered saline (PBS) + 0.05% Tween 20 and blocked with SEA BLOCK blocking buffer (Thermo Scientific) in the first panning round and 5% milk powder in PBS in the second panning round ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene, OR, US; Donkey anti-Mouse, AlexaFluor 488, A21202, Invitrogen, Eugene ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated for two hours with Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody against phospho-AKT and total-AKT and Alexa Fluor 596-conjugated goat anti-mouse secondary antibody for PRMT5 (diluted 1:100 in blocking buffer, Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... followed by permeabilization and blocking for 20 min with 0.3% Triton X-100 in 10% horse serum (Gibco, 16050-122). Primary antibodies were diluted in 10% horse serum and samples were incubated with the antibody overnight at +4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were blocked at room temperature for 30 min in blocking buffer (4% (wt/vol) UltraPure BSA (Thermo Fisher Scientific) in 2× SSC supplemented with 3% (vol/vol ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... goat anti-mouse 488 or goat anti-rabbit 488 diluted in blocking buffer (A11034 and A32731, Thermo Fisher; 1:400) for 75 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... in 1X PBS before staining with primary antibodies diluted at 1:400 ratio in 5% NGS blocking buffer and a mixture of 1:1000 of Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen) and 1:5000 DAPI (Sigma-Aldrich in 1X PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The next day sections were washed three times for 10 minutes in PBST followed by a 2-hour incubation in the dark with secondary antibodies in blocking solution (1:1000 donkey anti-rabbit Alexa Fluor 594 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were incubated in a blocking/permeabilization buffer (0.3% Triton X-100 and 5% normal goat serum (Fisher Scientific) in 1X PBS ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... cells were spun down and washed with PBS then resuspended in 1mL blocking solution with either 1:500 Goat anti-Mouse IgM Heavy Chain Secondary Antibody conjugated to AlexaFluor 488 (Invitrogen), 1:500 Rabbit anti Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... and anti-mouse IgG antibody conjugated with Alexa Fluor 568 dye (A-11019, Life Technologies; 1000x dilution in blocking solution) for 60 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were then incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse AlexaFluor 568 (Invitrogen, Carlsbad, CA) at 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 x 2 mins and blocked with SeaBlock blocking buffer (Thermo Fisher) for 15 mins at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with secondary antibodies diluted in blocking buffer for 45 min (1:1000 Alexa-fluor 546 conjugated to mouse or rabbit, Invitrogen) followed by three PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor secondary antibodies were diluted to 1:500 in blocking solution and included: 488 goat anti-rat (Invitrogen #A21212), 488 goat anti-chicken (Invitrogen #A11039) ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene, OR, US; Donkey anti-Mouse, AlexaFluor 488, A21202, Invitrogen, Eugene ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then washed in blocking buffer 2X for 2 hours and subsequently incubated with Goat anti-Mouse Alexa Fluor 555 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed three times in PBS and incubated in blocking solution containing an Alexa 488 conjugated secondary antibody (1:1500; Invitrogen), washed in PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated overnight at 4°C in blocking-permeabilizing solution containing antibodies anti-PAX6 (mouse, 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...