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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Recombinant Mouse Il1f6 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were developed with a mouse polyclonal anti-SmHDAC8 antibody (1/1000) or mouse polyclonal anti-SmRho1 antibody (1/1000) and peroxidase coupled anti-mouse secondary antibody (1/50 000; Invitrogen). Detection was carried out by chemiluminescence using SuperSignal™ West Dura Extended Duration Substrate (Thermo Scientific ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: Mouse forebrains fractions or HEK293T cell lysates were incubated with either mouse anti-CaMKIIα (Thermo Fisher Scientific catalog #MA1–048), rabbit anti-Shank3 (Bethyl catalog #A304-178A) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-Mcm3 antibody (M038-3; MBL) and anti-mouse IgG conjugated to Dynabeads M-280 (Thermo Fisher Scientific) were used as the primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Wells were consecutively washed two times for 5 min in washing buffer before incubation with secondary antibodies (Alexa Fluor-488 goat anti-mouse IgG, Invitrogen, A-11029; Alexa Fluor-647 goat anti-mouse IgG, Invitrogen, A-21236 ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488-conjugated goat anti-mouse-IgG antibodies and Alexa-Fluor-594-conjugated goat anti-mouse-IgG antibodies (Thermofisher) were used.
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bioRxiv - Microbiology 2020Quote: ... Samples were separated by SDS-PAGE and Western blotting carried out using a mouse mono- and polyubiquitinated ubiquitin conjugate (Ubiquigent) or mouse Ub-K63 (ThermoFisher) antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 hr at room temperature in the dark with secondary antibodies Alexa488-conjugated goat anti-mouse IgG and Alexa546-conjugated goat anti-mouse IgG (Invitrogen). Nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Additional labeling of C2C12 with MF-20 hybridoma mouse IgG2B primary antibody and Alexa Fluor 546 goat anti-mouse IgG2B secondary antibody (Invitrogen) was used to evaluate the cell differentiation into myotubes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the anti-BiP mouse monoclonal antibody and the anti-mouse secondary antibody were obtained from Invitrogen (Grand Island, NE, USA). Dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2022Quote: Cytokines in mouse lung homogenates were measured using a custom ProcartaPlexTM Mouse Mix & Match Panels kit (Invitrogen Waltham, MA, USA) on the Bio-Plex 200 (Bio-Rad Laboratories Ltd).
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bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa488- and 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa 488-/ 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec) and an AF647 conjugated donkey anti-mouse IgG antibody (Invitrogen). Following 3 washes with PBS containing 0.5% BSA ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... Secondary antibodies included goat anti-rabbit and goat anti-mouse from Novus conjugated to DyLight 650 or Janelia Fluor 646 and goat anti-mouse from ThermoFisher Scientific conjugated to Alexa Flour 488 for immunofluorescence ...
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bioRxiv - Physiology 2022Quote: ... Sections were then incubated with fluorescently conjugated secondary antibodies (Goat x Mouse IgM Alexa 350 and Goat x Mouse IgG Alexa 488, Invitrogen) and imaged using an inverted fluorescence microscope (Axio Observer ...
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bioRxiv - Immunology 2022Quote: ... the wells were incubated with PBS (control) or serially-diluted irrelevant mouse IgG (anti-green fluorescence protein of mouse IgG, ThermoFisher) or irrelevant rabbit IgG (anti-green fluorescence protein of rabbit IgG ...
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40, #124610, BioLegend; or PE anti-iNOS, #12592082, Invitrogen; or FITC anti-mouse CD8a ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies (Alexa fluor 546 goat anti-mouse IgG and Alexa fluor 488 goat anti-mouse IgG, Thermo Fisher Scientific) were incubated at 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: Larvae were dissected and stained as previously described23 with BRP (mouse BRPnc82, DSHB; diluted 1:200) and secondary Alexa Fluor 488-coupled goat anti-mouse (Invitrogen Cat#A-11001 ...
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bioRxiv - Physiology 2021Quote: ... We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix). The sections were counterstained with Nuclear Fast Red ...
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bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse IgG (H+ L) Alexa Fluor 555 and Goat anti-Mouse/Rabbit IgG (H+L) FITC was purchased from Invitrogen; Mouse anti-Flag/HA-tags beads was purchased from Abmart ...
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bioRxiv - Microbiology 2019Quote: ... for 1 hour at room temperature followed by anti-mouse IgG labeled with Alexa Fluor 488 (anti-mouse IgG-Alexa Fluor 488; Invitrogen) secondary antibodies and 4′,6′-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2021Quote: ... For immunofluorescence analysis the cells were fixed as described above and stained with a monoclonal mouse antibody against the viral capsid proteins followed by staining with a goat anti-mouse IgG Alexa Fluor 488 (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... Exogenous BMP on the cell surface was detected using the mouse anti-BMP 6C4 monoclonal antibody followed by incubation with goat anti-mouse IgG conjugated with AlexaFluor488 (Invitrogen).
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bioRxiv - Immunology 2020Quote: ... and detection antibody against mouse IgA (1 in 250) from the mouse total IgA ELISA kit from Invitrogen (CA, USA). The positive control (anti-S IgG ...
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bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA kits according to the manufacturer instructions (Mouse IFN gamma Uncoated ELISA, Invitrogen, #88-7314; Mouse TNF alpha Uncoated ELISA, Invitrogen, #88-7324 ...
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bioRxiv - Physiology 2021Quote: ... sections were washed in PBS 3 x 5 min and exposed to fluorescently conjugated secondary antibodies (60 min; Goat x Mouse IgM Alexa 350 and Goat x Mouse IgG Alexa 488, Invitrogen). Sections were then washed in PBS 3 x 5 min ...
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bioRxiv - Microbiology 2021Quote: ... Western blot analysis was performed using mouse anti-Spo0A (19) as the primary antibody and goat anti-mouse conjugated with Alexa 488 (Invitrogen) as the secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 hrs at room temperature with fluorescent secondary antibodies (anti-mouse Alexa Fluor 594, Invitrogen, #R37115; anti-mouse Alexa Fluor 488, Invitrogen, #A21202 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies including goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (31430 thermo) and anti-mouse IgA HRP-conjugated (626720 Invitrogen) were diluted 1:5000 ...
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bioRxiv - Biophysics 2020Quote: ... as then detected with mouse anti-myc (1:500) and visualized with donkey anti-mouse AF-488 (Invitrogen; 1:500). Nuclei were stained with propidium iodide (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... then incubacted 3 h with fluorescent-conjugated secondary antibodies diluted in saturation buffer (Goat anti-mouse IgG1-APC and Goat anti-mouse IgG2a A555 from Invitrogen). PCLS were washed 4 times with 300 μL of PBS (2x 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... the IgG subtype-specific secondary antibodies anti-mouse IgG1 AlexaFluor 488 and anti-mouse IgG2b AlexaFluor 647 (1:1000, Invitrogen) were used.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... then placed in secondary antibody (goat anti-mouse IgG2a, Alexa 488, 1:1000, ThermoFisher; goat anti-mouse IgG1, Alexa 647, 1:1000, ThermoFisher) diluted in PBS with 2% horse serum and incubated for 2 hr at 22°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were rinsed with PBS and incubated with goat anti-mouse Alexa Fluor 546 or goat anti-mouse Alexa Fluor 488 (Invitrogen) for one hour.
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bioRxiv - Genomics 2022Quote: Mouse ES cells were cultured on irradiated mouse embryonic fibroblasts (MEFs) under standard conditions: 500 ml DMEM (Gibco, 41965-039), 20% fetal bovine serum (Biological Industries ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies were detected with goat anti-mouse IgG2a AlexaFluor 647 and goat anti-mouse IgG1 AlexaFluor 568 (both Invitrogen). Cytoplasm was detected with HCS CellMask Green (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... mouse INFB1 (FAM, Mm00439552_s1) and Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited) (Thermofisher) and Applied biosystems Taqman™ Fast Universal PCR Master Mix (2x ...
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bioRxiv - Neuroscience 2024Quote: Mouse KIBRA and PICK1 genes were cloned from mouse tissue by RT-PCR and inserted into pCRII-TOPO (Invitrogen #K4600J10) or pCR-XL-TOPO vectors (Invitrogen #K475010) ...
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bioRxiv - Immunology 2024Quote: ... sections were blocked with 5% mouse serum and labelled using anti-mouse CD8 (clone 4SM15, Invitrogen cat 14-0808-82) followed by anti-rat IgG Alexa555 ...
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bioRxiv - Immunology 2024Quote: ... 14- 4301-85), and anti-IFNAR1 (mouse IgG1κ, 16-5945-85), its isotype control (mouse IgG1κ, 14- 4714-85) were from Invitrogen. Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were incubated with secondary antibodies diluted 1:1000 in 5% BSA in PBST (anti-mouse Alexa Fluor 555, CST 4409S; anti-mouse Alexa Fluor 647, Invitrogen, A-21235 ...
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bioRxiv - Microbiology 2023Quote: ... and Spo0A-3xFLAG was detected using mouse anti-Spo0A antibody.30 Goat anti-mouse IgG conjugated with Alexa 488 (Invitrogen) was used as a secondary antibody ...
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bioRxiv - Genetics 2023Quote: Cytokines in mouse lung homogenates were measured using a custom ProcartaPlexTM Mouse Mix & Match Panels kit (Invitrogen, Waltham, MA, USA) on the Bio-Plex 200 (Bio-Rad Laboratories Ltd.).