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Citations for Thermo Fisher :
3151 - 3200 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Membranes were hybridized at 42 °C with 5 nM of a 5’-biotinylated oligonucleotide probe (Table S4) in ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) and then washed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Transferred RNA was UV cross-linked and hybridized overnight with 100 ng/mL of 5’-biotinylated probes (Table S2) in ULTRAhyb hybridization buffer (Ambion) at 42 °C as described previously (27 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were incubated with biotinylated RBD, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidinphycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at ¼ concentration of the biotinylated RBD ...
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bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μl pre-mix (25 μl 0.4 mM Biotin-dATP (Invitrogen, #19524016), 25 μl 0.4 mM Biotin-dCTP (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Amplified and biotinylated sense-strand DNA targets were generated from total RNA using the WT Plus Kit (Thermo Fisher, MA). Fragmented and biotinylated sense-strand DNA target (5.5 µg ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... 300 μg of purified monoclonal antibodies were chemically biotinylated using a 20-fold molar excess of sulfo-NHS-biotin (ThermoFisher) for two hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2019Quote: ... mucins were also detected using biotinylated Ulex europaeus agglutinin I (UEA-I) lectin to detect fucose residues (Vector, Burlingame, CA) or with biotinylated maleimide to alkylate and detect reduced sulfhydryls (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biochemistry 2021Quote: ... briefly sonicated chromatin materials diluted using hybridization buffer and hybridized with 100pmol of biotinylated DNA probes targeting HOTTIP or LacZ containing 100 mL of Streptavidin-magnetic C1 beads (Invitrogen). RNA and DNA hybrids and RNA binding proteins were subjected to analysis by qRT-PCR and western blot with respective antibodies respectively ...
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bioRxiv - Biochemistry 2021Quote: ... that were hydrated for 10 min in assay buffer then plunged in a solution containing 1 μM 3′ end–biotinylated TAR RNA in assay buffer with 1x RNAase inhibitor (RNA Secure, Invitrogen) and 0.1 μM BSA for the RNA loading step ...
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bioRxiv - Biophysics 2021Quote: ... Purification was carried out exactly as for the non-biotinylated constructs and biotinylation was confirmed by western blot using NeutrAvidin-HRP (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... DNA was then recovered and sonicated to a length between 150 and 200 bp and biotinylated DNA fragments were purified using streptavidin beads (MyOne C1, Invitrogen). The DNA was then end-repaired and ligated to hairpin adaptor BU2 and amplified by PCR ...
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bioRxiv - Immunology 2021Quote: Immunoprecipitations for competition assays were performed with 8 μg of C-terminally biotinylated nanobody on M-280 streptavidin magnetic beads (Dynabeads, Invitrogen) and 10 μg of spike or RBD preincubated with 10 μg of the indicated (HIS-tagged ...
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bioRxiv - Immunology 2020Quote: ... Each well was washed and binding of biotinylated monoclonal antibodies was determined with a 1:30000 dilution of HRP-conjugated streptavidin (ThermoFisher). HRP was detected with TMB and stopped with 1% HCl ...
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bioRxiv - Biochemistry 2022Quote: ... About 1 pmol per sample of the biotinylated template DNA was captured on the streptavidin magnetic beads (Dinabeads MyOne streptavidin C1, Invitrogen) in buffer A (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated RNAs were pre-coupled to Nucleic Acid Compatible Streptavidin Magnetic Beads in RNA Capture Buffer (Pierce/Thermo Fisher Scientific) for 3h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The excess biotin of biotinylated mAbs was removed with 7k molecular weight-cutoff (MWCO) Zeba spin desalting columns (Thermo Scientific). Plates were coated with 2 μg/ml RBD antigen overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 5.8 μl of 1 mg/ml of PierceTM biotinylated Protein G (ThermoFisher Scientific, Waltham, MA; Cat#29988) and 20 μg of either the hnRNP H antibody (Bethyl ...
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bioRxiv - Physiology 2022Quote: ... 2×105 CD11b+ monocytic BM cells that were isolated by biotinylated anti-CD11b (1:100; clone M1/70; ThermoFisher Scientific) and anti-biotin microbeads (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... as well as ensure the specificity of streptavidin- purified preparations for biotinylated proteins via silver stain (Pierce, Thermo fisher #24612) and western blot.
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bioRxiv - Systems Biology 2024Quote: ... and 200 cycles per burst for 65 seconds) and biotinylated DNA fragments were pulled down using MyOne Streptavidin T1 beads (Invitrogen). End repair ...
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bioRxiv - Neuroscience 2024Quote: ... were injected with 0.2 µl of the anterograde axonal tracer biotinylated dextran amine (BDA, molecular weight 10 000, 0.1 µg/µl; Molecular Probes) at 0.2 µl/min into the ipsilesional stroked cortex at ...
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bioRxiv - Immunology 2024Quote: ... Becton Dickinson) along with biotinylated PvAMA1 using Streptavidin coupled with allophycocyanin (Becton Dickinson) and SYTOX Green Dead Cell Stain (Invitrogen) to gate out dead cells ...
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bioRxiv - Cell Biology 2024Quote: EV surface proteins were captured using biotinylated using Pierce™ Cell Surface Biotinylation and Isolation Kit (A44390, Thermo Fisher Scientific), digested with trypsin (Promega ...
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bioRxiv - Immunology 2024Quote: ... antigen proteins of the target were biotinylated using the EZ-link™Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher), then coupled to the fluorescent neutravidin beads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Pull-down of biotinylated DNA was performed with Dynabeads My One T1 Streptavidin beads (Thermo Fisher Scientific, catalog No.65601), and library preparation was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina ...
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bioRxiv - Synthetic Biology 2023Quote: ... the cells were incubated with biotinylated target, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at one-quarter of the concentration of the biotinylated targets ...
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bioRxiv - Immunology 2022Quote: Serum ELISAs were performed using randomly biotinylated SOSIP trimers using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: CEM WT and ADAM10 KO cells were biotinylated using the Pierce™ Cell Surface Biotinylation and Isolation Kit (Thermo Fisher). After biotinylation ...
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bioRxiv - Neuroscience 2023Quote: ... at varying depths was made targeting the deep layers of the primary motor cortex using 10% biotinylated dextran amine (BDA; 10,000 MW) (Life Technologies) (n=4 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vectastain Elite ABC kit ...
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bioRxiv - Microbiology 2023Quote: ... The EdU-labeled RNAs were then biotinylated and captured by using the Click-it Nascent RNA Capture Kit (Life Technologies), in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... using biotinylated RNA oligonucleotides (LGC biosearch technologies, Risskov, Denmark, sequences in supplements) immobilized on Dynabeads M-280 streptavidin magnetic beads (Invitrogen) and incubated with HeLa cell nuclear extract ...
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Pathology 2023Quote: Vip3Aa toxin was biotinylated with a 1:30 (protein:biotin) molar ratio of EZ-Link Sulfo- NHS-LC-Biotin (Life Technologies) for 30 minutes on ice ...
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bioRxiv - Synthetic Biology 2023Quote: ... a mixture of 3 GFP-specific biotinylated primers were used to capture GFP transcripts using Sera Mag Beads (Fisher Scientific). After a second round of DNase treatment ...
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bioRxiv - Microbiology 2023Quote: ... were biotinylated by mixing 50 μM of protein with 50 μM of EZ-Link™ Sulfo-NHS-Biotin (Thermofisher Scientific) followed by incubation on ice for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... The GDF-15 and GFP antibodies were biotinylated using an EZ-Link Micro NHS-PEG4-Biotinylation Kit from Thermo Fisher Scientific (21955 ...
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bioRxiv - Biochemistry 2022Quote: ... Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher) at room temperature prior to use.
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were then biotinylated by incubation with 1 mg/ml of the cell membrane-impermeable reagent EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher) dissolved in PBS for 30 minutes at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vecstatin Elite ABC kit ...
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bioRxiv - Bioengineering 2023Quote: The binding of the scFvs to biotinylated rHl and rLr (30 nM) was assessed using a DELFIA-based assay on black Maxisorp plates (Nunc), as previously described 32 ...
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bioRxiv - Immunology 2023Quote: ... 25 µg of Spike protein was biotinylated according to the instructions of EZ-LinkTM Micro Sulfo-NHS-LCBiotinylation Kit (ThermoFisher). Then fluorescent (APC ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were incubated with biotinylated Hsc70, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at 1/4 concentration of the biotinylated Hsc70 ...
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bioRxiv - Biochemistry 2023Quote: ... Hisα1-subCD3-WT (Legrand et al., 2020) was biotinylated with EZ-Link™ NHS-PEG4-Biotin (Thermo Scientific™ A39259) in a 1:1.5 ratio at 25°C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200, ThermoFisher Scientific A28181) antibody for two hours at room temperature and then a streptavidin conjugated Alexa-Fluor 488 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... or RSV antigen mix were biotinylated with EZ-Link Sulfo-NHS-LC-Biotin following manufacturer’s instructions and coupled to FluoSpheres NeutrAvidin beads (Molecular Probes) (16 hours ...
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bioRxiv - Genetics 2024Quote: ... Similar to SNP-seq, 500 ng of different alleles (31 bp, biotinylated) from a SNPs were attached to 25 μl streptavidin-Dynabeads (Invitrogen) and then incubated with 100 μg of Daudi nuclear extract for 1 h with or without non-biotinylated competitor (the same alleles) ...