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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
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bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified using Coomassie Protein Assay Reagent (ThermoFisher). Protein samples (30 μg ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cell surface amino groups were biotinylated in PBSG by treating with membrane impermeant Sulfo-NHS-Biotin (Thermo Fisher Scientific) for 30 min on ice (1 mM final ...
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bioRxiv - Cell Biology 2020Quote: ... the Fibrinogen anchor in the above protocol was replaced by the protein of interest at a concentration of 50 μg/ml in 0.1 M sodium bicarbonate pH 8.3 (except for fluorescent EGF, where biotinylated EGF complexed to streptavidin-Alexa555 (Invitrogen, E35350) was pattered at 1 μg/ml in 0.1 M sodium bicarbonate ...
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bioRxiv - Immunology 2021Quote: ... The pool was incubated with biotinylated SARS CoV2 Spike extracellular domain bound to 5 μL streptavidin M280 dynabeads (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Genetics 2021Quote: ... 4sU-labelled RNA was thiol-specific biotinylated by addition of 200 μg EZ-link HPDP-biotin (ThermoFisher Scientific, 21341), biotinylation buffer (10 mM Hepes-KOH pH 7.5 and 1 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 nM biotinylated 685-bp DNA fragment (68% AT; Table S2) coupled to 3 μL M280 streptavidin dynabeads (ThermoFisher) was incubated with 5 nM prey DNA and H-NS in supplemented Filament Buffer at 5–8 μM 6:4 WT H-NS ...
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bioRxiv - Neuroscience 2021Quote: ... A ∼50% solution of either 3000 MW Tetramethylrhodamine biotinylated dextran or 10000 MW Alexa-488 conjugated dextran (Molecular Probes) was injected into the spinal cord immediately dorsal to the swim bladder using a PV820 pneumatic picospritzer (World Precision Instruments) ...
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bioRxiv - Genomics 2020Quote: ... 10% of biotinylated probes (by mass; e.g. 10 μg probes for 100 μg gDNA) were pre-coupled to Streptavidin beads (ThermoFisher) using the manufacturer recommended protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated RNA was purified also as described (Duffy and Simon, 2016) using 80 μl MyOne Streptavidin C1 Dynabeads (Invitrogen) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Neuroscience 2019Quote: ... EDE exosomes were enriched by sequential immunoprecipitation with two biotinylated monoclonal antibodies to CD31 (MEM-05, Thermo Fisher Scientific) and then CD146 (Novus Biologicals ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with the biotinylated secondary antibody (goat anti-mouse IgG Biotin, Life Technologies B-2763, 1:100) diluted in 0.1 % Triton X-100 in PBS for 1 h at RT in a humidified chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Digested DNA ends were filled-in and biotinylated with a nucleotide mix containing 0.4 mM biotin-14-dCTP (Invitrogen) instead of dCTP and 5 U/μl Klenow fragment Dpol I (NEB ...
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bioRxiv - Bioengineering 2021Quote: Biotinylated aptamer probes were attached to the following streptavidin conjugates: Streptavidin AlexaFluor™ 647 Conjugate (Thermo Fisher Scientific, S32357), Streptavidin APC Conjugate (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Purified lysozymes were then mono-biotinylated (Fleire & Batista, 2009) using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... biotinylated with 7:1 biotin-to-antibody using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (21435, Thermo Fisher Scientific), was prepared in dilution buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... after which ncRNA bound biotinylated depletion oligos were pulled down using Dynabeads™ MyOne™ Streptavidin C1 beads (Invitrogen), according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... Chemiluminescent detection of biotinylated DNA was carried out according to the manufacturer’s instruction (Thermo Scientific LightShift Chemiluminescence EMSA Kit).
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bioRxiv - Cell Biology 2021Quote: ... and the N-terminus of the construct was attached to a biotinylated DNA-coated paramagnetic bead (2.8 μm diameter, Invitrogen) via a biotin-neutravidin interaction ...
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bioRxiv - Biochemistry 2022Quote: SOSIP trimers were randomly biotinylated following manufacturer’s guidelines using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific). The Pierce Biotin kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... a PDL-coated mylar film dish was biotinylated by incubating NHS-biotin (Thermo Fisher Scientific, 200 4 µg/ml) for 3h ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 pmol RNAs were biotinylated using Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher Scientific, USA, Cat. No. 20160) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Biotinylated ligation ends were then pulled down using 10 μl of Dynabeads© MyOne™ Streptavidin C1 (Invitrogen, 650.01) per μg of DNA ...
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bioRxiv - Immunology 2022Quote: ... During this time the primary antibody was biotinylated according to manufacturer protocol (Thermo Scientific EZ-Link NHS-PEG4-Biotin). Blocking solution was removed and 100 μl/well of biotinylated mAb 1C7C7 ...
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bioRxiv - Molecular Biology 2022Quote: ... The size and integrity of the biotinylated RNA were checked on a 6% Urea gel (Thermo Scientific, Darmstadt, Germany). Immediately before use ...
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bioRxiv - Genomics 2019Quote: ... Each sample of biotinylated DNA was pulled down with 100μl Dynabeads MyOne Streptavidin C1 beads (Thermal Fisher Scientific, 65002) by incubating at room temperature for 15min ...
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bioRxiv - Genomics 2019Quote: ... The biotinylated primers and undigested library members were removed using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, Waltham, MA), using the recommended “Immobilize nucleic acids” protocol ...
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bioRxiv - Bioengineering 2019Quote: ... was biotinylated using a 5:1 molar excess of Ez-Link™ Sulfo-NHS-LC-Biotin (Thermo-Fisher Scientific) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated RNA was purified also as described (Duffy and Simon, 2016) using 80 μl MyOne Streptavidin C1 Dynabeads (Invitrogen) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Bioengineering 2019Quote: ... the prepared bacterial suspension was incubated with 10 µg/mL biotinylated Salmonella polyclonal antibody (Thermo Scientific, Waltham, MA, USA) on a vortex mixer at 500 rpm at room temperature for 1 hr ...
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bioRxiv - Immunology 2020Quote: ... S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher). Streptavidin-BV510 (BD ...
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bioRxiv - Genomics 2022Quote: Biotinylated informative DNA ligation products were pulled down using Dynabeads MyOne streptavidin C1 paramagnetic beads (Thermo Fisher cat. #65001). After thorough washing of the ligation products-beads complex and having the sample in 35.7μl of volume ...
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bioRxiv - Synthetic Biology 2022Quote: The RBDs were biotinylated by amine coupling with sulfo-NHS-SS-Biotin according to the manufacturer’s manual (Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of biotinylated Igs after desalting was measured at 280 nm via spectrophotometer (Nanodrop ND-1000, ThermoFisher Scientific); biotin incorporation was measured using the Pierce Biotin quantitation kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 5303 SPRN SPRN-5) was biotinylated using the EZ-Link NHS-PEG4 Biotinylation Kit (Thermo Scientific, catalog No. PI21455) and used at a concentration of 1µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... both RBD and HEL were site-specifically biotinylated in house using EZ-link maleimide-PEG2-biotin (ThermoFisher, CAT#A39261) so that the binding epitopes on these proteins were not modified ...
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bioRxiv - Molecular Biology 2023Quote: ... an amino acid analog of methionine containing an alkyne moiety that can be biotinylated by click-it chemistry (ThermoFisher), for nascent protein translation labeling followed by SDS-PAGE and HRP-streptavidin Western analysis ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated RNA was purified also as described (Duffy and Simon, 2016) using 80 μl MyOne Streptavidin C1 Dynabeads (Invitrogen) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hSA4 antibody (in-house antibody) was generated using 200 μg Sulfo-NHS-LC Biotin (Pierce, Thermo Fisher Scientific), diluted 1:200 in blocking buffer and added to the plate for 1 hour at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... sections were washed 3 times with PBS and incubated at room temperature with biotinylated universal secondary antibody (Life technologies). After washing with PBS x3 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated IgG retained on plates was reacted with a 1:10,000 dilution Streptavidin-HRP conjugate (Thermo Scientific, cat. N504) in ADA-WB containing 10 mg/ml BSA for one hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...