Labshake search
Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for 6 hydroxy 2 naphthalenesulfonic acid sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in Hanks’ Balanced Salt Solution (ThermoFisher Scientific Cat# 88284) to 12000 cells/uL for immediate downstream processing ...
-
bioRxiv - Cancer Biology 2023Quote: ... heparin salt (4 µg /ml) and pen/strep (1% v/v) (Invitrogen). Spheroids were allowed to grow for 5-10 days before being counted by a blinded observer ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated in pre-warmed EBSS (Earls Balanced Salt Solution) (Invitrogen) at 37°C for 4 hours to induce autophagy ...
-
bioRxiv - Neuroscience 2023Quote: ... in Hanks’ Balanced Salt Solution (HBSS) without calcium and magnesium (Gibco #14170161). Enzyme solution containing the tissue was immersed in a shaking water bath at 37 °C for 20 min followed by trituration with glass pipettes ...
-
bioRxiv - Bioengineering 2023Quote: ... Cascade Blue tracer dye (Cascade Blue® hydrazide, trilithium salt; ThermoFisher, C3239) was introduced to the apical medium at 50 μg/ml and perfused through the epithelial channel of the chip ...
-
bioRxiv - Microbiology 2023Quote: ... the transformation mix was diluted in Hanks’ balanced salt solution (HBSS; Gibco) and added to one well of the 6-well plate ...
-
bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 250-500 μL Hank’s balanced salt solution (Gibco, 14025092) containing 2% FBS ...
-
bioRxiv - Bioengineering 2024Quote: ... RAW264.7 cells were serially passaged using Hank’s Balanced Salt Solution (HBSS, Gibco) and cell scrapers (Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were suspended in Hanks’ balanced salt solution (Gibco, Thermo Fisher Scientific) with 5% fetal bovine serum and 2 mM EDTA ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were suspended in Hanks’ balanced salt solution (Gibco, Thermo Fisher Scientific) with 5% fetal bovine serum and 2 mM EDTA ...
-
bioRxiv - Microbiology 2024Quote: ... Parasites were pelleted and washed in Hank’s Balanced Salt Solution (HBSS, Gibco). Parasites were resuspended in lysis buffer (1% SDS ...
-
bioRxiv - Biochemistry 2024Quote: ... washed two more times with Hank’s balanced salt solution (HBSS, Thermo Scientific) and pelleted again ...
-
bioRxiv - Neuroscience 2024Quote: ... This luciferase then oxidates the luciferin compound (D-luciferin, monosodium salt, Thermofisher), which is administered to flies orally ...
-
bioRxiv - Synthetic Biology 2024Quote: ... by dissociation in Hank’s balanced salt solution with calcium and magnesium (Gibco). Cortical tissue was digested in papain according to the manufacturer’s protocol (Worthington) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peptides were reconstituted using 20 μl 0.1% formic acid and loaded on an Acclaim PepMap 100 Nano-Trap Column (100 μm x 2 cm, Thermo Fisher Scientific) packed with C18 particles (5 μm ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein samples were reconstituted in solvent A (water/ACN [98: 2 v/v] with 0.1% formic acid) and separated using a C18-PepMap column (Thermo Fisher Scientific) with a solvent gradient of 2–100% Buffer B (0.1% formic acid and 98% acetonitrile ...
-
bioRxiv - Molecular Biology 2021Quote: ... Peptides were diluted in 15 μl 0.1% formic acid and injected on an Acclaim PepMap 100 trap column (nanoViper C18, 2 cm length, 100 μM ID, Thermo Scientific). Separation was performed with an analytical EasySpray column (PepMap RSLC C18 ...
-
bioRxiv - Cancer Biology 2022Quote: ... After tryptic digestion of the proteins the resulting peptides were resuspended in sample loading buffer (2% acetonitrile and 0.05% trifluoroacetic acid) and were separated by an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific) coupled online to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2019Quote: ... The peptides from each fraction were reconstituted in 20 μL of 0.1% formic acid and loaded onto an Acclaim PepMap 100 Nano-Trap Column (100 μm × 2 cm, Thermo Fisher Scientific) packed with 5 μm C18 particles at a flow rate of 4 μL per minute ...
-
bioRxiv - Developmental Biology 2020Quote: ... developing a linear gradient from 2% to 80% acetonitrile in 0.1% formic acid within 60 minutes (RSLC nano, Dionex – Thermo Fisher Scientific) and analyzed by MS/MS ...
-
bioRxiv - Cancer Biology 2019Quote: ... 0.2% SDS) at 50°C for 60 min on a Thermomixer (1200 rpm) followed by extraction with acid phenol (ThermoFisher, AM9712) and precipitation with 1:1 isopropanol:ethanol ...
-
bioRxiv - Biochemistry 2021Quote: ... using solvent A (0.1% formic acid in water) and separated on a C18 PepMap RSLC column (2 cm, 100 A; Thermo Fisher Scientific) using a linear gradient from 7 to 30% of solvent B (0.1% formic acid in acetonitrile ...
-
bioRxiv - Cancer Biology 2020Quote: Lungs and LNs were excised and immersed in 4% paraformaldehyde with 10% sucrose and 7% picric acid in PBS for 2 hours and then embedded in Tissue-Tec OCT (Fisher Scientific). 10 micron sections were prepared and slides were stained for B220 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 μm ID x 15 cm, 2 μm, Thermo Scientific) mounted on a DPV ion source (New Objective ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
-
Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
-
bioRxiv - Microbiology 2020Quote: Dried samples were reconstituted in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) for analysis on the Q-Exactive™ HF-X mass spectrometer (ThermoFisher Scientific), interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) at 300 nl/min via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: ... All data were normalized to the total protein content determined in protein pellets after neutralization with 10 N NaOH and sonication in 2% SDS plus 8 mM EDTA using a bicinchoninic acid assay (ThermoFisher Scientific) and bovine serum albumin as a standard.
-
bioRxiv - Immunology 2020Quote: ... tryptic peptides obtained from StageTip-based SCX fractionation were reconstituted in 0.1% formic acid and loaded on a nanoViper 2 cm (3 µm C18 Aq) trap column (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (50 cm ...
-
bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
-
bioRxiv - Microbiology 2022Quote: ... Amino acids were extracted on ice-cold lysis buffer [5:3:2 ratio of methanol-acetonitrile-water (Fisher Scientific, Pittsburgh, PA)] containing 3 µM of amino acid standards [Cambridge Isotope Laboratories ...
-
bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 12 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: A codon-optimized gene encoding for SARS-CoV-2 (331 to 528 amino acids, QIS60558.1) was expressed in Expi293 cells (Thermo Fisher Scientific) with human serum albumin secretion signal sequence and fusion tags (6xHistidine tag ...
-
bioRxiv - Plant Biology 2019Quote: ... They were then diluted 10x with loading buffer (2% acetonitrile, 0.05% trifluoroacetic acid) and injected on a Fusion Tribrid orbitrap mass spectrometer (Thermo Fisher Scientific) interfaced to a Dionex RSLC 3000 nano-HPLC ...
-
bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
-
bioRxiv - Genomics 2020Quote: ... The resuspended peptides in sample loading buffer (2% acetonitrile and 0.05% trifluoroacetic acid) were separated and analysed on an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific) coupled online to either a Q Exactive HF or an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Then the pellets were resuspended in 10 mL of sterile water and transferred to a 15 mL falcon tube containing 2 g of acid washed glass beads (0.5 mm of diameter, Thermofisher, G8772-100G). Cells were then disrupted using a Fastprep system (MP Biomedicals) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
-
bioRxiv - Biophysics 2022Quote: ... Electron microscopy grids were prepared in 2% (w/v) phosphotungstic acid (pH 7.5) and were examined in a Talos L120C transmission electron microscope (FEI-Thermo Fisher Scientific). Nanodisc samples were frozen in aliquots and stored at −80°C.
-
bioRxiv - Microbiology 2022Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 17 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
-
bioRxiv - Pathology 2024Quote: ... the peptide was reconstituted in 2% acetonitrile/0.1% formic acid (FA) and analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2024Quote: Dried peptides were suspended in 0.1% formic acid (FA) with 2% ACN and analyzed by LC-MS/MS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) connected to an EASY nLC 1200 (buffer A ...
-
bioRxiv - Cell Biology 2024Quote: ... iRBCs were washed twice with media to remove extracellular merozoites before being aliquoted in triplicate into a 96-well U-bottomed plate and stained with the nucleic acid dye SYTO-61 (2 μM) (Thermo Fisher) for 15 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
-
bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
-
bioRxiv - Microbiology 2023Quote: C18 purified digests were re-suspended in Buffer A* (2% acetonitrile, 0.01% trifluoroacetic acid) and separated on a Ultimate 3000 UPLC (Thermo Fisher Scientific) with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and a PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Eclipse Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface both at 300 nl/minute via the analytical columns using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...