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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA in the protein samples was sheared by sonication and the amount of protein was estimated using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated on 4–20% Mini-PROTEAN® TGX™ Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™, PI23227). The desirable amount of the protein lysate (15 – 30 μg ...
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bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014, respectively) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein concentration was determined using the Bicinchoninic Acid protein assay (Thermo Scientific, US). Lysates (3μg/μl in 1xSDS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was adjusted using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For co-immunoprecipitation assay ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins separated by SDS-PAGE were stained with PageBlue Protein Staining Solution (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), before extracts were stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were estimated using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific# 23227). 400-500μg of protein from the stably transfected HEK293 cells were used to immunoprecipitate FLAG-tagged MIB1 using EZview™ Red ANTI-FLAG® M2 affinity gel (MilliporeSigma# F2426 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit (Thermofisher Scientific, 23225) colorimetric reaction and quantified on a SpectraMax i3 Multi-Mode Platform (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... 40uL of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) were added to each well and then incubated at 4ºC for 4 hours on a rotator ...
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bioRxiv - Immunology 2021Quote: ... Total protein concentration was assessed using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of whole-cell protein lysates samples were boiled in Laemmli loading buffer ...
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bioRxiv - Genetics 2021Quote: ... Protein amounts were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein (in vitro samples ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined via the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted with Tissue Protein extraction reagent (T-PER, Thermofisher Scientific, USA) + Protease inhibitor cocktail (Pierce #A32961) ...
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bioRxiv - Neuroscience 2020Quote: ... Data were normalized to total protein concentration as determined by BCA protein assay (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: ... protein concentration of lysates was measured using Pierce™ BCA Protein Assay Kit (ThermoFisher), to ensure equal loading of proteins ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts (100 µg of protein) were incubated with streptavidin agarose beads (Thermo Scientific) for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were measured using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). Cytoplasmic and nuclear samples from each brain region containing 5μg total protein was boiled in Laemmli loading buffer and run on a 4%–20% Bio-Rad precast gel ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein content was quantified with a Pierce BCA Protein Assay Kit (Thermo Scientific). The lysate [50 µg protein/50 µL] was incubated with 0.5 µL of 1 M DTT solution at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher). 50 μg of protein was loaded into each lane of Mini-Protean TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein amount was confirmed with a BCA protein assay kit (Thermo Fisher Scientific). Proteins were reduced by treatment with 10 mM DTT for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific), then 30 μg protein lysates were mixed with NuPAGE™ LDS Sample Buffer (4x ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Protein gels were stained with Coomassie blue (PageBlue Protein Staining Solution; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured with Pierce BCA Protein concentration assay kit (Thermo Fisher Scientific) on a spectrophotometer plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: ... Protein content was determined with the BCA Protein Assay kit (23225, Thermo Fisher Scientific). Equal amounts of protein were resolved on gels under reducing conditions ...
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bioRxiv - Genomics 2020Quote: ... Total protein content was quantified by Qubit™ Protein Assay Kit (Thermo Fisher Scientific). Sap containing 100 ug of protein was freeze-dried and resuspended in 5% SDS and 50mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration was measured by Pierce Coomassie (Bradford) protein assay kit (ThermoFisher Scientific, 23200) and the Turner spectrophotometer SP-830.
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bioRxiv - Genomics 2020Quote: ... Protein content was measured using the Pierce BCA protein assay (Thermo Scientific, Warrington, UK). For Western Blot analysis 30 μg of protein were loaded on a 10% 1mm SDS-PAGE gel ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was determined utilizing Pierce 660 Protein Assay kit (Thermo Fisher Scientific, 22662) with ionic detergent compatibility reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Scientific). Disulfide bridges were reduced with 5 mM tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Physiology 2019Quote: ... Total protein was quantified using the Pierce Micro BCA Protein Assay Kit (Thermo Scientific). 8 µg of total protein was separated by electrophoresis on a precast 4-15% TGX gradient gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A (for H3K27ac and H3K27me3) or Protein G (for 8WG16) Dynabeads (ThermoFisher Scientific) were blocked overnight at 4°C in RIPA buffer without protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... before measuring the protein content using Micro BCA™ protein assay kit (ThermoFisher, USA). The viral/nanoparticle corona complexes were then resuspended in PBS and adjusted with ammonium bicarbonate to a final concentration of 20 mM ...
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bioRxiv - Microbiology 2019Quote: ... Total protein was quantitated by fluorometry using the Qbit Protein Assay Kit (Thermo Fisher). Beta-lactamase activity was determined by the addition of 80 ng nitrocefin to either 250 ng or 1000 ng of total protein ...
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bioRxiv - Microbiology 2019Quote: ... Tissue lysates were normalized for protein content with Pierce 660nm Protein Assay (Thermo Scientific), boiled in NuPAGE LDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... overnight followed by incubation with sepharose protein G beads (Dynabeads Protein G, Life Technologies). Precipitates and input DNA were analyzed by qRT-PCR using PRACR3-fw/rv oligonucleotides listed in Supplementary Table S2 ...
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bioRxiv - Cancer Biology 2020Quote: ... protein lysates were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated by SDS–PAGE and transferred to nitrocellulose or PVDF membranes using the Trans-blot Turbo Transfer System (BioRad ...
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bioRxiv - Biophysics 2019Quote: ... protein concentrations were evaluated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific) and 10 µg of total protein was loaded into a 16% SDS-PAGE gel ...