Rabbit Anti Borrelia burgdorferi sensu stricto B31 DbpB Antibody citations for Thermo Fisher

Tau phosphorylated at serine 356 is associated with Alzheimer’s disease pathology and can be lowered in mouse and human brain tissue using the NUAK inhibitor WZ4003

Lewis W. Taylor, et al., bioRxiv - Neuroscience 2023

Quote: ... Sections were washed with 0.1 M PBS containing 0.3% triton X-100 then incubated in secondary antibodies (donkey anti-rabbit Alexa-fluor 594, Invitrogen A21207 and donkey anti chicken Alexa-fluor 647, Invitrogen A78952) diluted 1:200 in block buffer at room temperature for 1 hour ...

ALK signalling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition

Marcus Borenäs, et al., bioRxiv - Cancer Biology 2023

Quote: ... Horseradish peroxidase– conjugated secondary antibodies, goat anti-mouse immunoglobulin G (IgG) (# 32230), and goat anti-rabbit IgG (# 32260, 1:5000) were purchased from Thermo Fisher Scientific ...

Cooperative activities of dICA69^N-BAR and dCIP4^F-BAR domain proteins regulate membrane tubule organization in vivo

Bhagaban Mallik, Sajad Bhat, Vimlesh Kumar, bioRxiv - Cell Biology 2023

Quote: ... Anti-mouse and anti-rabbit Alexa fluor 488/568 conjugated secondary antibodies were incubated at 1:800 dilutions (Thermo Fisher Scientific). The cells were mounted in Vectashield (Vector Laboratory ...

Selective inhibitors of the Aurora A-TPX2 protein-protein interaction exhibit in vivo efficacy as targeted anti-mitotic agents

Simon R. Stockwell, et al., bioRxiv - Biochemistry 2023

Quote: ... followed by probing with secondary antibodies (goat anti-rabbit Alexafluor 488, A11034, 1:500; goat anti-rat Alexafluor 647, A21247, 1:500, ThermoFisher Scientific) applied and washed as per the primary antibodies ...

Endolysosomal dysfunction in radial glia progenitor cells leads to defective cerebral angiogenesis and compromised Blood-Brain Barrier integrity

Ivan Bassi, et al., bioRxiv - Developmental Biology 2023

Quote: ... embryos were incubated overnight at 4°C with secondary antibodies conjugated with Alexa Fluor 633 (1:500; Goat anti-Rabbit Invitrogen A21070, Goat anti-Mouse Invitrogen A21053).

Sequence independent activity of a predicted long disordered segment of the human papillomavirus L2 capsid protein during virus entry

Changin Oh, et al., bioRxiv - Biochemistry 2023

Quote: ... Cells were then incubated at RT for 2 hours with 1:1000 Alexa Fluor (AF) 647 donkey anti-mouse and 1:200 of AF488 donkey anti-rabbit fluorescence conjugated secondary antibodies (Life Technologies). The cover slip was mounted in mounting solution with DAPI ...

WRNIP1 PREVENTS G4/R-LOOP-ASSOCIATED GENOMIC INSTABILITY

Pasquale Valenzisi, et al., bioRxiv - Cell Biology 2022

Quote: ... and incubated with the specific secondary antibody: goat anti-mouse Alexa Fluor-488 or goat anti-rabbit Alexa Fluor-594 (Molecular Probes). The incubation with secondary antibodies were accomplished in a humidified chamber for 1 h at RT ...

3D Co-culture Model on the Role of Vimentin in Notch Signaling and Vascularization

Khalil Dayekh, Kibret Mequanint, bioRxiv - Bioengineering 2023

Quote: ... then incubated with the corresponding secondary antibody (Alexa-488 conjugated goat anti-mouse and Alexa-594 conjugated goat anti-rabbit (Life Technologies)) (1:150 ...

Extracellular communication between brain cells through functional transfer of Cre mRNA

David Rufino-Ramos, et al., bioRxiv - Cell Biology 2023

Quote: ... the membranes were washed 3 times in TBS-T for 10 minutes each and incubated with an alkaline phosphatase-linked secondary goat anti-mouse/anti-rabbit antibody (1:10,000; Thermo Scientific Pierce) at RT for 1h ...

Signaling protein abundance modulates the strength of the Spindle Assembly Checkpoint

Chu Chen, et al., bioRxiv - Cell Biology 2023

Quote: ... the coverslips were washed 4 times with PHEM containing 0.05% Tween-20 and incubated with appropriate Alexa Fluor 633-conjugated secondary antibodies (Goat anti-Mouse, Invitrogen A21052 or Goat anti-Rabbit, Invitrogen A21071) at a 1:10000 dilution for 45 min at room temperature in the dark ...

Oxidative stress-induced MMP- and γ-secretase-dependent VE-cadherin processing is modulated by the proteasome and BMP9/10

Caterina Ivaldo, et al., bioRxiv - Cell Biology 2022

Quote: ... antibodies were used followed by specific secondary Alexa Fluor antibodies (1:500 anti-mouse Alexa Fluor 546 and 1:500 anti-rabbit Alexa 488 Thermo Fisher). For actin staining ...

LIM-HD transcription factors are required for regeneration of neuronal and intestinal cell subtypes in planarians

M. Dolores Molina, et al., bioRxiv - Developmental Biology 2023

Quote: ... The secondary antibodies Alexa 488-conjugated goat anti-mouse and Alexa-568-conjugated goat anti-rabbit (Molecular Probes, Waltham, MA, USA) were diluted 1:400 and 1:1000 ...

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage C. elegans embryos

Pierre Gönczy, Fernando R. Balestra, bioRxiv - Developmental Biology 2023

Quote: ... slides were incubated 45-60 min at room temperature with secondary antibodies in PBT (1:500 goat anti-rabbit-Alexa488 and goat anti-mouse-Alexa568 (ThermoFisher Scientific). Slides were counterstained with 1 μg/ml Hoechst 33258 (Sigma ...

Pharmacological Induction of mesenchymal-epithelial transition chemosensitizes breast cancer cells and prevents metastatic progression

Meisam Bagheri, et al., bioRxiv - Cancer Biology 2023

Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...

Vimentin filaments integrate low complexity domains in a highly complex helical structure

Matthias Eibauer, et al., bioRxiv - Biochemistry 2023

Quote: ... in PBS containing 5% normal goat serum for 30 min at RT followed by incubation with goat anti-chicken and anti-rabbit secondary antibodies (1:400, A-11039, A-11011, Invitrogen, USA) for 30 min at RT ...

TREM2 mediates MHCII-associated CD4⁺ T cell response against gliomas

Jiaying Zheng, et al., bioRxiv - Neuroscience 2023

Quote: ... Slides were incubated with secondary antibodies of goat-anti-rat 488 and goat-anti-rabbit 594 (1:500, Invitrogen, A11006, A11037) 1.5 hours in room temperature ...

Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID

Andrea Bernardini, et al., bioRxiv - Molecular Biology 2023

Quote: ... with AF488-conjiugated secondary antibodies diluted 1:3000 in BPS (goat anti-mouse IgG, A11001 or goat anti-rabbit IgG, A11008, Life Technologies). For dual-color IF ...

Extensive remodelling of XIST regulatory networks during primate evolution

Emmanuel Cazottes, et al., bioRxiv - Genomics 2023

Quote: ... washed with 1XPBS then incubated for 40 min at RT with Alexa Fluor 488 nm anti-rabbit or Alexa Fluor 594 nm anti-mouse secondary antibodies (Life Technologies). Cells were then washed in 1XPBS and mounted in Vectashield plus DAPI (Vector Laboratories) ...

XIST dampens X chromosome activity in a SPEN-dependent manner during early human development

Charbel Alfeghaly, et al., bioRxiv - Molecular Biology 2023

Quote: ... cells were incubated for 40 min at RT with Alexa Fluor 488 nm anti-rabbit or Alexa Fluor 594 nm anti-mouse secondary antibodies (Life Technologies). Finally ...

ST8Sia2 polysialyltransferase protects against infection by Trypanosoma cruzi

Bruno Rafael Barboza, et al., bioRxiv - Microbiology 2023

Quote: ... the cells were incubated for 1 h at RT with the secondary antibodies: goat anti-rabbit immunoglobulin G (IgG) Alexa Fluor 488 and/or goat anti-mouse IgG Alexa Fluor 594 (Thermo Fisher Scientific ...

Highly variable molecular signatures of TDP-43 loss of function are associated with nuclear pore complex injury in a population study of sporadic ALS patient iPSNs

Jeffrey D. Rothstein, et al., bioRxiv - Neuroscience 2023

Quote: ... were used as primary antibodies and Goat anti-rabbit Alexa 647 and goat anti-guinea pig Alexa 568 (Thermo Fisher Scientific) were used as secondary antibodies ...

NK cells contribute to resistance to anti-PD1 therapy in immune-excluded melanomas

Ewout Landeloos, et al., bioRxiv - Cancer Biology 2023

Quote: ... Sections were washed in DPBS for 10 min and incubated with secondary antibodies (Donkey anti-rabbit Life Technologies cat. no A31573, 1:400; Donkey anti-rat ThermoFisher Scientific cat ...

Endogenous oncogenic KRAS expression increases cell proliferation and motility in near-diploid hTERT RPE-1 cells

Naushin L. Hindul, et al., bioRxiv - Cell Biology 2023

Quote: ... 40D4 Mouse mAb #2920, dilution 1/2000), anti-pAKT (Cell Signalling Technology, rabbit Antibody #9271, dilution 1/1000),anti-ERK (Thermo-Fisher Scientific: Invitrogen, mouse antibody #10221703 ...

Endogenous oncogenic KRAS expression increases cell proliferation and motility in near-diploid hTERT RPE-1 cells

Naushin L. Hindul, et al., bioRxiv - Cell Biology 2023

Quote: ... 40D4 Mouse mAb #2920, dilution 1/2000), anti-pAKT (Cell Signalling Technology, rabbit Antibody #9271, dilution 1/1000),anti-ERK (Thermo-Fisher Scientific: Invitrogen ...

Influenza infected macrophages release viral ribonucleoproteins that shape the local host response

Svenja Fritzlar, et al., bioRxiv - Microbiology 2023

Quote: ... Membranes were washed in PBS-T and incubated with fluorescent secondary antibody (anti-mouse or anti-rabbit conjugated to AlexaFluor488 or 647, both Life Technologies) for 2-3h at RT ...

Excitatory effects of dentate gyrus mossy cells on granule cells and area CA3: an vitro and in vivo study in adult mice

Hannah L. Bernstein, et al., bioRxiv - Neuroscience 2023

Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...

EPIGENETIC MODULATION VIA THE C-TERMINAL TAIL OF H2A.Z

László Imre, et al., bioRxiv - Cell Biology 2023

Quote: ... using Alexa fluor 488 (A488) or Alexa fluor 647 (A647) conjugated goat anti-mouse IgG or goat anti-rabbit IgG antibodies (Thermo Fisher Scientific ...

Inhibition of striatal indirect pathway during second postnatal week leads to long lasting deficits in motivated behavior

Pedro R. Olivetti, et al., bioRxiv - Animal Behavior and Cognition 2024

Quote: ... Alexa Fluor-conjugated secondary antibodies (donkey anti-rabbit Alexa Fluor-546 and goat anti-chicken Alexa Fluor-488, Invitrogen, 1:1000) were used for fluorescent signal detection and incubated for 1 hour at room temperature ...

T_reg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastoma

Xiaodan Qin, et al., bioRxiv - Cancer Biology 2023

Quote: ... Secondary antibodies included horseradish-peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:2000 or 1:5000; ThermoFisher, 31430 or 31460). Proteins were detected by ECL chemiluminescence detection kits (ThermoFisher ...

Pharmacological cAMP stimulation via prostaglandin receptors rescues ciliary defects in CEP290-deficient human and mouse models

France de Malglaive, et al., bioRxiv - Genetics 2023

Quote: ... the sections were washed three times with PBS 1X and then incubated for 1 hour with donkey anti-mouse (or anti-rabbit) Alexa Fluor 488nm (or 555nm) secondary antibodies (1:200, Thermofisher Scientific). DAPI (Roche ...

A single cell atlas of the mouse seminal vesicle

Fengyun Sun, et al., bioRxiv - Genomics 2024

Quote: ... and incubated with either anti-rabbit or anti-rat secondary antibodies with AF594 (diluted 1:400, A11012 or A1107, Thermofisher Scientific) for 1 hour ...

Global regulation via modulation of ribosome pausing by the ABC-F protein EttA

Farès Ousalem, et al., bioRxiv - Microbiology 2024

Quote: ... of skimmed milk powder and then incubated for 2 h at RT or overnight at 4 °C in 3 ml of PBS 1X + 0.1% (v/v) Tween-20 in presence of a 1,000x dilution of anti GFP antibody (Rabbit anti-GFP, Invitrogen Thermo Fisher Scientific). After washing ...

GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

Quote: ... and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) and HRP conjugated Goat anti-mouse IgM (#31440) for immunoblotting were from Thermo Fisher. APC conjugated anti-human ganglioside GD2 antibody (#357306 ...

Passage of the HIV capsid cracks the nuclear pore

Jan Philipp Kreysing, et al., bioRxiv - Biophysics 2024

Quote: ... secondary antibodies donkey anti-rabbit IgG and donkey anti-mouse IgG conjugated with Alexa Fluor 488 and 647 (Thermo Fisher Scientific), respectively ...

Inhibition of EGFR/ErbB does not protect against C. difficile toxin B

Uswah Siddiqi, et al., bioRxiv - Microbiology 2024

Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).

Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1 (CTR1/SLC31A1)

Rachel Curnock, Peter J. Cullen, bioRxiv - Cell Biology 2020

Quote: ... goat anti-rabbit 800 (Invitrogen).

Poly(ADP-ribose)-binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesions

Ramhari Kumbhar, et al., bioRxiv - Cell Biology 2020

Quote: ... rabbit anti-GFP (A11122; Invitrogen), rabbit anti-PARP1 (9542 ...

ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatiotemporal control of the endolysosomal pathway

Tom Cremer, et al., bioRxiv - Cell Biology 2020

Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...

Potent but transient immunosuppression of T-cells is a general feature of CD71⁺ erythroid cells

Tomasz M. Grzywa, et al., bioRxiv - Immunology 2021

Quote: ... goat anti-rabbit IgG (Invitrogen).