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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Detection of proteins was performed using secondary antibodies conjugated to horseradish peroxidase-HRP and the super signal west pico chemiluminescent substrate (Thermo Scientific-Pierce).
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitations (IPs) were also performed from the same lysates with the (Neuromab) antibody mAb-α-iFGF14 conjugated to protein G dynabeads (Life Technologies), using previously described methods (Bosch et al. ...
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bioRxiv - Plant Biology 2024Quote: ... The remaining supernatant was subjected to immunoprecipitation by adding 5 μg anti-Flag antibody conjugated with Dynabeads™ Protein A (Thermo Fisher) and incubated at 4℃ for 16 h ...
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bioRxiv - Neuroscience 2024Quote: ... 500 μg protein was incubated with rabbit IgG control or rabbit anti-tau antibody overnight at 4°C and pulled down by protein A/G Magnetic Agarose beads (Thermo Scientific, 88802) for 6 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Genomics 2024Quote: ... The cell-bead conjugates were then resuspended in recommended buffer with 0.5 μg antibody targeting the protein of interest: Anti-BPTF (ThermoFisher Scientific A300-973A), Anti- SRF (Cell Signaling Technology 5147S) ...
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bioRxiv - Plant Biology 2024Quote: Yeast proteins were extracted (71) and LjVIH2KD was visualized via immunodetection with a primary mouse anti-V5 antibody (Invitrogen, 1:2000 dilution) followed by a secondary Alexa Fluor™ Plus 680 goat anti-mouse antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbits were immunised with the purified protein. The rabbit serum (no. 2481) was used together with anti-rabbit-HRP antibody (Thermo Scientific, 31460) in 5% BSA/TBST for ChmA detection in immunoblotting ...
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bioRxiv - Immunology 2024Quote: ... diluted antibodies against cell surface proteins (see Supplementary Table 2) and a viability dye (LIVE/DEAD Fixable Blue, Thermo Fisher Scientific, #L23105) were added ...
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bioRxiv - Cancer Biology 2024Quote: ... total lysates containing 1 mg of protein were incubated with 1 µg of a rabbit polyclonal anti-PRMT5 antibody overnight at 4°C and were then incubated with 20 µl of Protein A/G agarose (22851, Thermo Fisher Scientific) for 3 hours at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 350 µg clarified lysate at 0.4 mg/ml was then subjected to immunoprecipitation for 75 min with a pan-anti-Ago antibody (4F9,[19]) pre-bound to Pierce protein-L magnetic beads (Thermo Scientific, #88849); 21 µg Ab ...
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bioRxiv - Cell Biology 2021Quote: Samples were run in NuPAGE Bis-Tris gels (4%-12% [Invitrogen NP0321] for small volumes of samples, 10% [Invitrogen NP0315] for larger amounts) in 1x MOPS SDS running buffer (Invitrogen NP0001 ...
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bioRxiv - Cancer Biology 2024Quote: ... Biobanking and long-term storage of GBOs25,83 was performed by transferring small GBO pieces (0.1 – 0.2 mm3) to GBO culture medium containing 10% DMSO in cryogenic vials (Thermo Fisher Scientific, 13-700-504). Vials were stored in a foam Cell Freezing Container (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Testicular tissues were cut into small pieces of 1–2 mm and digested in 10 mL DMEM: F12 media (Gibco, Waltham, MA, USA) containing 1 mg/mL type IV collagenase for 15 min on a rotor at 37°C ...
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bioRxiv - Physiology 2024Quote: ... were exposed to controlled vortices generated by the spinning movements of small encapsulated (coated) magnetic stir bars (6 x 3mm, Fischerbrand, #11888882, Fisher scientific, Leicestershire, UK) inside the dishes ...
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bioRxiv - Physiology 2024Quote: ... and exposed to controlled vortices generated by the spinning movements of small encapsulated (coated) magnetic stir bars (6 x 3 mm, Fisherbrand, #11888882, Fisher Scientific, Leicestershire, UK) inside the dishes ...
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bioRxiv - Neuroscience 2024Quote: Whole brains isolated from 10-month-old mice were immediately minced into small pieces with a sterile surgical blade in ice-cold Hanks’ balanced salt solution (HBSS) (Thermo Fisher Scientific, #14175095). Tissue chunks were dissociated by mechanical homogenization in 10 mL 1X HBSS containing 15 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... or IL-2 combined with spike protein for 16 hrs and used for RNA extraction using TRIzol™ reagent (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... We measured the RNA integrity using Agilent RNA screentape and RNA concentration using Qubit RNA HS assay kit (ThermoFisher Scientific). We used 1.2 µg total RNA for mRNA purification ...
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bioRxiv - Immunology 2021Quote: ... Fluorometric quantification of RNA and RNA integrity analysis was carried out using RNA Biochip and Qubit RNA Fluorescence Dye (Invitrogen). cDNA libraries were generated using NEBNext® Ultra™ II Directional (stranded ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorometric quantification of RNA and RNA integrity analysis was carried out using RNA Biochip and Qubit RNA Fluorescence Dye (Invitrogen). cDNA libraries were generated using NEBNext® Ultra™ II Directional (stranded ...
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bioRxiv - Cell Biology 2021Quote: ... RNA quality was assessed with the RNA Qubit™ RNA HS Assay Kit (ThermoFisher). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted with RNA extraction kit (Invitrogen), and quantified at Nanodrop ...
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bioRxiv - Microbiology 2020Quote: ... RNA was fragmented with RNA Fragmentation reagents (Invitrogen). Fragmentation reagent was added ...
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bioRxiv - Molecular Biology 2022Quote: ... Cellular RNA was stabilised using RNA-later (Invitrogen) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and RNA were quantified (Qubit RNA HS, Invitrogen) and validated for quality (Agilent Bioanalyzer 2100) ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured using a protein assay kit (Pierce™ BCA Protein Assay Kit, #23225, ThermoFisher Scientific, Cheshire, UK). Protein samples were resolved through 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing proteins was collected and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific). Immunoblotting analysis was carried out as previously described.(8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... following which protein-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader.
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was measured through the Bicinchoninic Acid protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™ 23225). Equal amounts of proteins extracts (20-30 μg for mitochondrial samples or 40 μg for total kidney homogenates ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Protein extraction was performed as described previously37 and protein concentration was determined using Pierce BCA protein assay kit (Thermo scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader ...
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bioRxiv - Biochemistry 2023Quote: ... Dried proteins were reconstituted in 0.2 % SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 μg protein was incubated in 0.5% SLS and 1 μg of trypsin (Serva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Protein purity and concentration were determined using Coomassie Imperial Protein Stain and Pierce bicinchoninic acid protein assay (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Total protein quantification was determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific Pierce™ BCA Protein Assay Kit) and samples were stored at -80°C until use.
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bioRxiv - Pathology 2023Quote: ... aortic protein lysates were prepared in RIPA lysis buffer and protein concentration was measured using the BCA Gold protein assay (ThermoFisher). Equal protein amounts (25μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were sonicated and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, cat.no. 23225) for subsequent analyses.
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bioRxiv - Microbiology 2024Quote: ... Dried proteins were reconstituted in 0.2% SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 µg protein was incubated in 0.5% SLS and 1 µg of trypsin (Serva ...
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bioRxiv - Molecular Biology 2021Quote: ... for 60 min followed by another digestion using 1000 U/ml collagenase type 2 and 11 U/ml dispase (Gibco, cat# 17105-041) for 30 min ...
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bioRxiv - Microbiology 2020Quote: 50µg of peptides from each sample were labeled with isobaric tandem-mass-tag (TMT) 11 plex reagents (Thermo Fisher Scientific, San Jose, CA) in 20 mM pH 8.5 HEPES with 30% acetonitrile (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sample with washing buffer (PBS, 1% Bovine Serum Albumin (BSA), cells were centrifuged (10 min, 150g, RT) and stained for microglia marker CD11b (Invitrogen, 11-0112-81) (1:100) ...
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bioRxiv - Neuroscience 2022Quote: ... HPCs in suspension were collected and spun down at 300 xG for 6 minutes and resuspended into microglia basal media (MBM, 2mL/well) containing: DMEM/F12 no phenol (Gibco # 11-039-021), 2% Insulin Transferin Selenite (Gibco # 41400045) ...