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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for Human Cathepsin B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... human embryonic kidney 293A cells (Invitrogen) were transfected with pHelper ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human FoxP3 EF450 (Invitrogen 48477642). Cells were stained with viability dye Zombie NIRtrade Fixable Viability Kit (Biolegend Inc ...
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bioRxiv - Microbiology 2024Quote: ... Heat stable recombinant human bFGF (Gibco) at 5U/ml was supplemented to organoid formation media only on day 0 ...
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bioRxiv - Microbiology 2020Quote: ... Protein extraction and determination was performed using the B-PER™ Bacterial Protein Extraction Reagent and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively.
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bioRxiv - Cancer Biology 2020Quote: ... were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher). Libraries were then purified via solid phase reversible immobilization (SPRI ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Immunology 2021Quote: ... The remaining cells were enriched for CD8+ cells with Dynabeads™ Untouched™ Human CD8 T Cells Kit (Thermo Fisher #11348D) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: DNA encoding the SNAP-Tag was PCR-amplified from the pSNAPf vector (NEBs, #N9183S) to be inserted in frame into the pT7CFE1-CHis plasmid provided in the human IVT kit (ThermoFisher, #88882). The Gateway cloning cassette was introduced immediately after the SNAP tag sequence using the Gateway vector conversion system (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 600 ng total RNA of human primary mesenchymal stem cells were reversely transcribed using the RevertAid RT Reverse Transcription Kit (K1691, Thermo Scientific) according to the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid DNA construct was used to transfect Expi293F human cells using the Expi293F transfection kit and Expi293F expression medium (Thermo Fisher). Cells were harvested by centrifugation at 1,000 g for 30 min and resuspended in 1 x PBS supplemented with 30 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell death analysis was performed by staining primary human hepatocytes with Dead Cell Apoptosis Kit with Annexin V FITC and PI (V13242, Thermo Fisher). PI+ve cells were then quantified with the flow cytometer (Fortessa ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... The poly(A)-tailed RNA in human CCRF-SB cells was isolated from the total RNA using a Fasttrack MAG Maxi mRNA isolation kit (Life Technologies), whereas the poly(A)-tailed RNA in yeast cells and mouse tissues was isolated from the total RNA using a Dynabeads mRNA Purification kit (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies) followed by Lithium Chloride precipitation and quantitation using a NanoDrop (Thermo Fisher Scientific)
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Immunology 2020Quote: ... Secreted factors were measured in the T cell assay culture media using a Luminex kit (Cytokine Human Magnetic 30 Plex Panel, Thermo Scientific) per manufacturer’s recommendations with 4 replicates per sample ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2.5 µg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes [65] using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... primed NK cells from PWH were cultured with autologous CD4+ T cells also isolated by negative immunomagnetic selection (DynaBeads Human CD4+ T cell isolation kit, Life technologies) in the presence of 50nM Romidepsin (Selleck Chemicals ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20 ...
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bioRxiv - Biophysics 2023Quote: ... University of Belgrade-Faculty of Biology which was previously produced by several reactions of cloning and subcloning of CTG repeats originating from human DMPK locus using pJet1.2/blunt cloning vector from CloneJET PCR Cloning Kit (Thermo Fisher Scientific). The subcloned region sequence was verified with Sanger sequencing before prior use ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the CD8-depleted PBMC fraction using the MagniSort human CD4+ T cell enrichment kit (Thermo Fisher). T cell purity was routinely checked by flow cytometry (>80%) ...
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Cancer Biology 2023Quote: ... human NRP-1 (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 using an Ab labeling kit (Molecular Probes, A20186). Images were acquired with an LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Biophysics 2023Quote: ... inducible Flp-In T-Rex-293 cell line expressing human RyR2 was generated using the Flp-In T-REx Core Kit (Invitrogen, USA) as described before (54) ...
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bioRxiv - Developmental Biology 2023Quote: 100 ng total RNA was reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression Kit following the manufacturer’s protocol (Thermo Fisher Scientific). Target genes (SQSTM1 ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was isolated from tissue lysates as well as from cultured human and murine aortic or DA SMCs with the PureLink RNA Mini Kit (ThermoFisher, 12183018). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: Human liver specimens were minced to extract genomic DNAs (gDNAs) using the PureLink™ Genomic DNA Mini Kit (Invitrogen™, K182001), and genomic DNA concentrations were measured using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Biophysics 2021Quote: ... The assay was then developed using 1-Step Ultra TMB-ELISA substrate solution (Thermo Scientific). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed before developed using either the SuperSignal ELISA Femto Substrate (Thermo Fisher Scientific) or ELC Prime Western Blotting system (Sigma-Aldrich ...