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Citations for Thermo Fisher :
3051 - 3100 of 3952 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: MCF-10A cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 Ham 1:1 powder (Gibco) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... from day 0 to day 2 the media was switched to a Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM F12, Gibco) based medium ...
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bioRxiv - Bioengineering 2023Quote: ... we washed the particles with PBSP (PBS with 0.5% w/v Pluronic F-127) before incubating with 10µg ml-1 streptavidin (ThermoFisher Scientific) for 30 minutes ...
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bioRxiv - Physiology 2023Quote: ... Skin tissues were shaved and cleaned with 70% ethanol then minced and incubated in DMEM/F-12 medium (ThermoFisher) with Liberase (Millipore Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... organoids were passaged using H+++B Medium (Advanced DMEM/F-12 [Thermo Fisher], HEPES pH7.2-7.5 [10mM, Thermo Fisher] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells between passages 12-24 were cultivated in DMEM/Ham’s F-12 medium (1:1 Mix) supplemented with L-glutamine and 15 mM HEPES (Gibco™ ...
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bioRxiv - Plant Biology 2023Quote: ... Detection of F-ara-EdU was performed using a Click-iT EdU Alexa Fluor 555 Imaging Kit (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM)/Hams F-12 nutrient mix (F12) GlutaMAXTM media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2024Quote: Non-transformed human mammary epithelial cells (MCF-10A, ATCC) were maintained in growth medium consisting of DME medium/Ham’s F-12 (ThermoFisher) containing HEPES and L-glutamine supplemented with 5% (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... LUHMES cells were grown at 37°C in a 5% CO2 atmosphere in DMEM/F-12 (Gibco 11330-032) containing N2 supplement (Gibco 17502048 ...
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bioRxiv - Molecular Biology 2023Quote: The human RPE cell line ARPE-19 was cultured in DMEM/F-12 (Cat #C11330500BT, Gibco, Waltham, MA, USA) media with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... The cultures were incubated for 2 h at 37°C with 5% CO2 and subsequently washed three times with Advanced Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 (Gibco)) ...
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bioRxiv - Immunology 2023Quote: Wild-type (WT) CHO-K1 cells and all stable CHO transfectant cells were grown in Ham’s F-12 medium (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... at 5,000g and 4°C following buffer-exchange in PBS (pH 7.4) with the inclusion of non-ionic surfactant Pluronic F-68 (cat#. 24040032, Gibco) (final storage solution ...
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bioRxiv - Bioengineering 2023Quote: ... The hydrogels were washed with PBS and incubated with both rhodamine phalloidin to visualize F-actin (1:600, Invitrogen) and secondary antibody (AlexaFluor 488 goat anti-mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... TrypLE reaction was quenched via dilution with cold Splitting Media (Advanced DMEM/F-12 [Gibco], 10 mM HEPES [Invitrogen] ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed three times followed by staining for F-actin (1:1,000; Alexa Fluor™ 546 Phalloidin, Invitrogen), and nuclei (DAPI ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... a TECAN infinite f plex plate reader and black 96-well luciferase plates (Thermo Fisher Scientific Nunc A/S).
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... a TECAN infinite f plex plate reader and black 96-well luciferase plates (Thermo Fisher Scientific Nunc A/S).
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bioRxiv - Cell Biology 2023Quote: Mouse primary myoblasts were isolated from the limb muscles of 2-3 day-old pups and cultured in Ham’s F-10 nutrient mixture (Invitrogen) as described before.38 The culture medium was supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... containing 3 mL of home-made Expansion Medium consisting of a 1:1 mixture of DMEM/F-12 (Gibco) and Neurobasal Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... rAAVs were then buffer-exchanged and concentrated using Millipore Amicon filter unit (UFC910008, 100K MWCO) with 1x DPBS containing 0.001% Pluronic F-68 (Gibco). AAV titer was quantified by measuring DNase-resistant genome copies using “Free-ITR” qPCR method with specific primers detecting WPRE element in AAV vectors ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Genomics 2023Quote: ... 25 mL of wash solution (DMEM/F-12 supplemented with 0.03% GIBCO BSA Fraction V, pre-warmed at 37C) was added to each plate to inactivate the trypsin ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells were transitioned to CNCC early maintenance media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 1X penicillin-streptomycin solution ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with PBS for 5 minutes at RT and F-actin was stained with Phalloidin-AF555 (Invitrogen) in Blocking buffer for 30 minutes at RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... BL21-AI [F-ompT hsdSB(r - m -) gal dcm araB::T7RNAP-tetA] was purchased commercially (ThermoFisher Scientific, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Induction into neural crest iPericytes was achieved by culturing in DMEM/F-12 plus GlutaMAX (Thermofisher Scientific, cat.#10565018) supplemented with 0.5% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting PM suspensions were then diluted with high-glucose Dulbecco’s Modified Eagle Medium /Nutrient Mixture F-12 (CAS 31331-028, DMEM/F12, Gibco) to reach a PM concentration of 1 mg mL−1 and sonicated for an additional 5 min ...
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bioRxiv - Cancer Biology 2024Quote: Mouse mesothelioma cell lines with stable Cas9 were previously generated in our laboratory and cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12+Glutamax; Gibco), supplemented with 4 μg/ml Hydrocortisone (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... the expansion medium was replaced with the following differentiation medium: Neurobasal plus DMEM/Ham’s F-12 media with l-glutamine 1:1 (Gibco), N2 supplement 1x (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... GCT cell lines were routinely maintained at 37°C with 5% CO2 in complete medium consisting of Dulbecco’s modified Eagle medium: nutrient mixture F-12 (DMEM/F12) (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: ... and F-actin was labeled with phalloidin conjugated Alexa Fluor 488 (Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA) dissolved in PBS buffer (1:200 ...
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bioRxiv - Developmental Biology 2024Quote: Retinas were cultured on 13 mm/0.2 micron floating filters (Cytiva, 10417001) on DMEM/F-12 media (Gibco, 11320082) with 10% Fetal Bovine Serum (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... Human precision cut lung slices (PCLS) were purchased from IIVS and were grown in DMEM/F-12 media (Gibco) supplemented with antibiotics and antimycotic reagents.
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bioRxiv - Neuroscience 2024Quote: ... The explants were stained for F-actin with Alexa Fluor 568 Phalloidin (1:400 dilution, A12380, Thermo Fisher Scientific) or antibody anti-pERK1/2 (Thr202/Tyr204 ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μg of AM dye was dissolved in 30 μL of DMSO/Pluronic F-127 (80/20; ThermoFisher Scientific) and stored in 4 μL aliquots at −20°C ...
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bioRxiv - Neuroscience 2024Quote: ... The hair cells were stained for F-actin with rhodamine-conjugated phalloidin (1:100, Rhodamine Phalloidin, Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... NSCs were maintained and expanded in NS expansion medium composed as follows: DMEM/Ham’s F-12 media with l-glutamine (Gibco), Glucose solution 29mM (Signa-Merck) ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488-coupled phalloidin was also used at a 1/500 dilution to label filamentous (F)-actin (ThermoFisher).
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Parental A549 cells (BEI Resources #NR-52268) were cultured in Ham’s F-12 K (Kaighn’s) medium (Fisher Scientific #21127030) with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Transgenic CHO cells were maintained in an F-12 Nutrient Mixture with GlutamaxTM-I (Thermo Fisher Scientific, MA, USA), 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... A similar protocol was followed for small scale virus production when establishing individual stable cell lines with transfection being performed on 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) reagent.
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bioRxiv - Microbiology 2021Quote: ... Each 10-μl RT-qPCR mixture consisted of 2.5 µL 4X TaqMan Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA), 400 nM each primer ...
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bioRxiv - Cell Biology 2021Quote: ... the extracellular RSV virus were collected by PEG6000 precipitation method and intracellular RSV virus were collected by lysing the macrophage cells with the lysis buffer from mirVanaTM miRNA Isolation Kit (Ambion, Life Technologies, AM1560). Total RNA from RSV infected macrophage cells as well as stock virus was extracted using mirVanaTM miRNA Isolation Kit according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: The full-length cDNA for HA or a mutated gene and neuraminidase genes of influenza A/Switzerland/9715293/2013 (H3N2) virus were amplified by using SuperScript One-Step RT-PCR (Invitrogen, Grand Island, NY), and the 6:2 recombinant viruses with six internal genes of influenza A/PR/8/1934(H1N1 ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Immunology 2020Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes of hexokinase 2 (HK2 ...
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bioRxiv - Immunology 2020Quote: 0.5 - 1 μg of total RNA was prepared by column purification (MachereyNagel) and transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (RevertAid Reverse Transcriptase, Thermo Fisher Scientific, #EP0441) in a total volume of 10 or 20 μl ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...