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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... we performed a Click reaction according to the manufacturer’s instructions to reveal the EdU or EU (Click-iT EdU Alexa Fluor 488 imaging kit; Click-iT RNA Alexa Fluor 488 imaging kit, both from Invitrogen) to label nascent DNA or RNA respectively.
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthesized by in vitro transcription with 1μl of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). the pCS2+_ancBE4max-SpymacCas9 has been linearized using KpnI restriction enzyme ...
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bioRxiv - Neuroscience 2020Quote: A lysis reaction followed by reverse transcription was performed using the kit Taqman Gene Expression Cells-to-CT Kit (ThermoFisher) with ‘Lysis Solution’ followed by the ‘Stop Solution’ at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: The PCR products were purified to remove unincorporated primers and dNTP’s using the PCR purification kit (Purelink PCR purification kit, Invitrogen, USA). The purified products were processed for sequencing on a next generation sequencer platform @ 40X coverage (MiSeq ...
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Nulliparity affects the expression of a limited number of genes and pathways in Day 8 equine embryosbioRxiv - Developmental Biology 2022Quote: ... RNA was extracted later from each hemi-embryo using PicoPure RNA isolation kit (PicoPure RNA isolation kit, Applied Biosystems, France), which included a DNAse treatment ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the Macherey Nagel Nucleo Spin Tissue DNA kit and quantified using a Qubit fluorometer (Qubit dsDNA BR Assay Kit, Q32853, Invitrogen). Thirty-seven blood samples at T0 (pre-stress ...
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bioRxiv - Genetics 2019Quote: ... using the Ion PGM™ sequencing 200 Kit v2 and the Ion 314™ Chip Kit v2 (Thermo Fisher Scientific). Data from the PGM runs were processed using the Ion Torrent Suite 5.4 software to generate sequencing reads ...
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bioRxiv - Molecular Biology 2019Quote: ... we used either the nickel-based protocol or the Pierce Cobalt kit (Pull-Down PolyHis Protein:Protein Interaction Kit cat. #21277 from Thermo Fisher). Similar results were obtained using either the nickel or cobalt protocol ...
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bioRxiv - Immunology 2020Quote: ... The plasmids were purified using standard plasmid kits and prepared for Sanger sequencing using BigDye Terminator v3.1 Cycle Sequencing Kit (ThermoFisher # 4337455) with pSB_EF1a_seq.FOR and pSB_bGH_seq.REV primers ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA was bar-coded using the Ion Xpress Barcode Adapters kit and quantified by quantitative PCR using the Ion Library TaqMan Quantitation Kit (ThermoFisher) after purification of libraries by Agencourt AMPure XP beads (Beckman) ...
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was assessed using the Click-iT TUNEL Alexa Fluor 546 Imaging Assay kit and EdU incorporation by Click-iT EdU Alexa Fluor 546 Imaging Assay kit (both from ThermoFisher), according to the manufacturers’ instructions.
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bioRxiv - Pathology 2021Quote: ... Apoptosis was assessed using the Click-iT TUNEL Alexa Fluor 546 Imaging Assay kit and EdU incorporation by Click-iT EdU Alexa Fluor 546 Imaging Assay kit (both from ThermoFisher), according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured using a protein assay kit (Pierce™ BCA Protein Assay Kit, #23225, ThermoFisher Scientific, Cheshire, UK). Protein samples were resolved through 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Neuroscience 2021Quote: ... SP was labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers nCoV_IP2-12669Fw ...
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bioRxiv - Plant Biology 2021Quote: ... Targets were prepared with a cDNA synthesis kit followed by biotin labeling with the IVT labeling kit (GeneChip 39IVT Express; Affymetrix) and hybridized to the ATH1 gene chip for 16h as recommended by the supplier ...
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bioRxiv - Microbiology 2019Quote: ... using the Ion PGM Sequencing 400 Kit and deposited in two Ion 318 chip Kit v2 following the manufacturer’s protocol (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: RNA extraction was done using Qiagen RNeasy kit and equal amounts of RNA were reverse transcribed using the Maxima Reverse Transcriptase kit (ThermoFisher). Semi-quantitative qPCR was performed using PerfeCTa SYBR Green SuperMix (QuantaBio) ...
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bioRxiv - Cancer Biology 2022Quote: ... reagent/kit and reverse transcribed to complementary DNA (cDNA) using a High Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, #4368813). The expression of PYGL (assay ID ...
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA was extracted using a commercial kit (PureLink™ RNA Mini Kit, Ambion, Life Technologies, Carlsbad, CA, USA). After RNA extraction ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA was extracted using a commercial kit (PureLink™ RNA Mini Kit, Ambion, Life Technologies, Carlsbad, CA, USA). After RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the RNeasy Mini Kit and treated with the DNase from the Turbo DNA-free Kit (Invitrogen) according to the manufacturer’s ‘rigorous DNase treatment’ protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and quantity was assessed using Agilent BioAnalyzer RNA 6000 Pico Kit and the Invitrogen Qubit RNA HS Assay kit (Invitrogen), respectively ...
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bioRxiv - Genetics 2022Quote: ... The pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Fisher Scientific, 78840) or resuspended in 1x RIPA buffer with protease inhibitors to extract whole cell lysate.
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bioRxiv - Microbiology 2019Quote: ... The pooled samples were cleaned with the MagBio Beads Cleanup Kit (MagBio) for a second time and quantified again using the dsDNA HS Assay kit for Qubit fluorometer (Invitrogen). The resulting library was sent to Eurofins genomics (Konstanz ...
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bioRxiv - Cell Biology 2019Quote: ... The cell pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Scientific, 78840), or resuspended in 1 x RIPA buffer with protease inhibitors to extract whole cell lysate ...
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bioRxiv - Cell Biology 2019Quote: ... All cells were authenticated by STR profiling (GenePrint 10 System kit from Promega and AuthentiFiler PCR Amplification Kit from ThermoFisher) and tested negative for mycoplasma contamination using the Cell Culture Contamination Detection Kit (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader.
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend], Zombie NIR fixable viability kit [Biolegend], eBioscience fixable viability dye eFluor 780 [Invitrogen]). Samples were analysed using a BD X-20 flow cytometer and data analysis was performed using FlowJo v10 (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend], Zombie NIR fixable viability kit [Biolegend], eBioscience fixable viability dye eFluor 780 [Invitrogen]). Samples were analysed using a BD X-20 flow cytometer and data analysis was performed using FlowJo v10 (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: ... The viability of bacteria in the particulate matters was studied using a live/dead viability kit (L7012 BacLight Viability Kit, Invitrogen) as described in Supplementary Text ...
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bioRxiv - Genetics 2020Quote: Total RNA was isolated from 5 × 106 log-stage parasites with the NEB Monarch Total RNA miniprep kit and contaminating genomic DNA was eliminated with the TURBO DNA-free kit from ThermoFisher. Samples were then subjected to first-strand cDNA synthesis using the Applied Biosystems High Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Genetics 2020Quote: ... Detection was done by either Western Breeze chromogenic kit or chemiluminescence kit (Pierce™ ECL Western Blotting Substrate, Thermo Fisher) using Kodak BioMax Light film.
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bioRxiv - Genetics 2020Quote: ... and mRNA was synthesized using an in vitro RNA transcription kit (mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit, AM1345, Invitrogen). sgRNAs were amplified and transcribed in vitro using the MEGAshortscript™ T7 Transcription Kit (AM1354 ...
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bioRxiv - Cell Biology 2020Quote: ... NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For GFP-centrin ...
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bioRxiv - Neuroscience 2021Quote: Cell viability was determined using a commercially available dual-wavelength fluorescent kit (LIVE/DEAD Viability/Cytotoxicity Assay Kit; L3224; Invitrogen). Live cells are detected by intracellular esterase activity using the dye calcein-AM (∼495/515 nm ex/em ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from 1 mL of this outgrown culture using a GeneJet Genomic DNA Purification Kit (ThermoScientific) and the concentration of genomic DNA was quantified using a Qubit dsDNA HS Assay Kit (Invitrogen). A 1 μg aliquot of genomic DNA was suspended in 15 μL of 10 mM Tris buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were transcribed with the MEGAshortscript T7 Kit from synthetic DNA oligomers and purified with the MEGAclear Transcription Clean-Up Kit (both ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... nuclear soluble and chromatin-bound protein fractions were extracted from cells using the Subcellular Protein Fractionation Kit for Cultured Cells kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJet Plasmid Miniprep kit (Thermo K0503) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Genomics 2021Quote: ... total RNA was extracted from DIII pearlfish embryos with a Qiagen RNeasy mini plus kit and reverse transcribed with a superscript IV VILO kit from ThermoFisher according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers ORF1ab_Fw ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from pooled 7-day old seedlings using the Qiagen RNeasy Plant Mini Kit and complementary DNA was synthesized using the Maxima cDNA Synthesis kit (Thermofisher). Quantitative RT-PCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... and nuclear compartments using consecutive centrifugation steps of varying speeds and specialized buffers obtained from a commercially available kit (Sub-Cellular Protein Fractionation Kit for Tissues, ThermoFisher). Bradford protein assays were performed for each compartment individually ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was removed by the DNase treatment kit (DNA-free™ DNA Removal Kit, Invitrogen, Thermo Fisher Scientific, Massachusetts, USA). Parasites were synchronized by 5% sorbitol (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... ABE8e or ABE9 mRNA was transcribed using the in vitro RNA transcription kit (mMESSAGE mMACHINE® T7 Ultra Kit, Ambion). mRNA was purified with MEGAclear™ Kit (Ambion) ...