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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Peptide analyses (corresponding to 1 µg protein) were performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) connected to an EASY-nLC 1200 ultra-HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 5.8 μl of 1 mg/ml of PierceTM biotinylated Protein G (ThermoFisher Scientific, Waltham, MA; Cat#29988) and 20 μg of either the hnRNP H antibody (Bethyl ...
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bioRxiv - Developmental Biology 2024Quote: ... A mixture of hydrophilic and hydrophobic magnetic beads was used to clean-up the proteins at a ratio of 20:1 beads:proteins (Sera-Mag Speed beads, Fisher Scientific). After addition of ACN to a final concentration of 50% ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transiently transfected at 50-60% confluency with plasmid expressing protein of interest (Table 1) and/or target specific siRNA using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: ... and C-X-C Motif Chemokine Ligand 1 (CXCL1) levels were measured in liver protein lysates and EDTA-plasma by enzyme-linked immunosorbent assay (ELISA) (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
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bioRxiv - Immunology 2024Quote: Total IgG was isolated from 1 mL plasma via affinity chromatography using Protein G Plus Agarose (Pierce Thermo Fisher Scientific) and cleaved into F(ab’)2 fragments using IdeS ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were first dialyzed into buffer containing 1 mM TCEP and then incubated with AlexaFluor 594 C5-maleimide (Invitrogen) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Plant Biology 2023Quote: ... The reduced CAM2-His6 protein was then mixed with 1 mg EZ-Link™ Maleimide Activated HRP (Thermo Fisher Scientific) in a molar ratio of 1:1 and incubated overnight at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Cell lysates was denatured under reducing conditions by boiling 30 μg of total protein with 1 M DTT and 4X LDS sample buffer (Invitrogen) at 95°C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The cornea samples were further incubated with a primary antibody targeting a tight junction marker protein (ZO-1, #40-2200, Invitrogen) at 4°C for 48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were measured using Quick Start Bradford 1× Dye Reagent (Bio- Rad®) on a NanoDrop2000 (Thermo Fisher Scientific) or DC Protein-Assay (Bio-Rad® ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were measured by BCA and samples were diluted to 1 mL of 0.5 mg/mL in RIPA buffer in low protein binding collection tubes (Thermo Fisher) containing 25 μL of NanoLink® magnetic streptavidin beads ...
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bioRxiv - Cell Biology 2023Quote: Blockage of EV HSP70 protein was performed by the pre-incubation of EVs with 1 μg/ml anti-HSP70 antibody (Invitrogen) [32] in high glucose DMEM (4.5 g/l ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatin preclearing was performed using IgG isotype (1 ug IgG/106 cells)-Protein G Agarose-coated beads (Thermo Fisher Scientific) mixed with chromatin ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates from WB5 lysis buffer were adjusted to the same protein concentration before incubating them with 1/50 (volbeads/vollysate) equilibrated NeutrAvidin-agarose beads (ThermoFisher) over-night at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Protein expression was monitored by immunoblotting with a 1:1000 dilution of Anti V5 Antibody (Fisher Scientific Catalog # R960-25) or a 1:1000 dilution of Anti-GFP polyclonal Antibody (Product # PA1-980A) ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with an anti-TasA antibody (rabbit) at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (Thermo Fisher). A secondary anti-rabbit IgG antibody conjugated to horseradish peroxidase (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... Sonicated chromatin was incubated overnight with 6 μg of C/EBPβ or IgG antibody (listed in Table 1) followed by 2h incubation with Dynabeads Protein G beads (Invitrogen). After five washes with LiCl buffer (100mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Each serum sample analyzed in this study was first passed through a 1 mL Protein G agarose (Thermo Fisher, 20397) affinity column in gravity mode ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by overnight incubation with conjugated anti-green fluorescent protein (anti-GFP) rabbit antibody (1:2000; catalog no. A21311; Invitrogen). Finally ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with ice-cold 1×DPBS and lysed in M-PER mammalian protein extraction reagent (ThermoFisher, Prod#78505) supplement with protease inhibitor cocktail (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: ... antibodies were added at a 1/500 dilution for the incubation step and their immobilization was performed using Dynabeads Protein G (Invitrogen). The IP products were washed six times with PBS prior to sRNA extraction using the Trizol reagent (Ambion) ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and a maximum amount of proteins (2.3-4 mg) was loaded on 1 mg of Dynabeads M-280 Streptavidin (ThermoFisher Scientific), previously equilibrated with RIPA buffer ...
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bioRxiv - Microbiology 2024Quote: ... 1 s pulses on low output) and protein concentrations were determined using a reducing agent-compatible BCA assay (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were then transferred into fresh Eppendorfs and frozen at −80°C (for radiolabeled glucose to protein) or their protein concentrations calculated (Western blot) using a Pierce BCA Protein Assay kit (Thermo Scientific, Cat#23225, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Neuroscience 2023Quote: Total protein levels in cortex homogenates were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Protein amounts were determined using a Qubit4 Fluorometer and Qubit Protein Assay kit (Thermo Fisher Scientific). GUS activity was calculated in (nmol 4-MU ...
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bioRxiv - Microbiology 2020Quote: ... Lysate protein concentrations were determined using a BCA protein assay kit according to manufacturer’s instructions (ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected for protein quantification using Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: Changes in protein expression were monitored using Click-iT Protein Synthesis Assay kit (Thermo Fisher Scientific). Oocytes and 2 cell embryos were cultured in M16 medium with the methionine analogue HPG at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and DNA-protein complexes were pulled down using Protein A/G agarose beads (Thermo Fisher, 20423). Agarose beads were washed with the following buffers ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined in the supernatant by BCA Protein Assay Kit (Thermo Fisher Scientific, #23227). 100 μg of protein was transferred into a new tube and the final volume was adjusted to 100 μL with 100 mM triethylammonium bicarbonate (TEAB) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants were collected for measuring the protein concentration with BCA Protein Assay Kit (ThermoFisher Scientific). Cytosolic and nuclear extracts were prepared using NE-PER reagents (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the supernatant measured using the Pierce BCA protein assay kit (Thermo Scientific) was used as a control.
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bioRxiv - Immunology 2021Quote: ... and sample protein concentration determined using the Pierce® BCA Protein Assay Kit (Thermo scientific, UK). SDS-PAGE was then performed ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were mixed with 20 μL Protein G-conjugated magnetic beads (DB10009, Thermo Fisher Scientific) with 2.0 μg antibody ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration for each sample was determined using the Pierce BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Supernatants were collected and protein quantification was performed using a BCA protein assay kit (Thermo Scientific). A total of 2,000 μg of protein extract was used to perform the immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... The extracted proteins were quantified by Pierce™ Modified Lowry Protein Assay Kit (Thermo Fisher Scientific) prior to precipitation by the phenol/chloroform method (Wessel and Flügge 1984) ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate total protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific, 23225).
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bioRxiv - Evolutionary Biology 2021Quote: ... Total protein extracts were prepared by using B-PER® Bacterial Protein Extraction Reagent (Thermo Scientific) as per manual ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of pooled triplicates was determined using the BCA protein assay (Thermo Fisher Scientific) and 8 μg of total protein subjected to immunoblotting against cleaved caspase-3 and −7 (1:200 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration was determined with the BCA Protein Assay Kit (Thermo Fisher Scientific, Shanghai, China).
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were assessed for total protein using the BCA protein quantitation kit (Thermo Fisher Scientific, UK). 15 μg of protein were separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: Oocyte surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific), modified here for oocytes as previously described (Lynagh ...