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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... One μg of total RNA was subjected to reverse transcription using Superscript III transcriptase (Invitrogen). The qRT-PCR was conducted using a Bio-Rad CFX96 system with SYBR green to determine the expression level ...
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bioRxiv - Neuroscience 2019Quote: ... One µg of RNA was used to synthetize cDNA using M-MLV reverse transcriptase (Invitrogen) with oligo-dT and random hexamers ...
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bioRxiv - Cancer Biology 2019Quote: ... Blots were blocked for one hour with 3% (weight/volume) bovine serum albumin (Fisher Scientific) and incubated overnight at 4° C with a rabbit monoclonal antibody to RHAMM [EPR4055] antibody at 1:1,000 dilution (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Real-time PCR was performed using the Step-One Real-Time PCR system (Applied Biosystems) using ChIP sample at different DNA dilutions (5 µL) ...
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Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... One microliter of amplification was mixed with 8.5 μL Hi-Di deionized formamide (Applied Biosystems), and 0.5 μL GeneScan 500 LIZ (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: The quantity and quality of RNA was assessed with a Nanodrop One Spectrophotometer (Thermo Fisher) and electrophoresis on a 1% agarose gel ...
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bioRxiv - Biochemistry 2020Quote: ... Real-time PCR was done on the Step One Real-Time PCR system (Applied Biosystems) using SYBR Green PCR Master Mix ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatant was then combined with one-half volume of Total Exosome Isolation Reagent (Invitrogen) and collected using the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA purity and concentration were measured using the NanoDrop UV Spectrophotometer (ThermoFisher ND-ONE-W). cDNA was generated with the SuperScript IV First-Strand Synthesis System (ThermoFisher 18091150 ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... FF fragments (each with a precursor for one of heteromismatches) were synthesised by Life Technologies, and cloned into plasmid pT2 using Gibson Assembly Cloning Kit (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were passaged by washing one time with phosphate-buffered saline solution (PBS; Gibco) and detached using 0.25% trypsin/2.21mM EDTA (Corning).
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bioRxiv - Cancer Biology 2020Quote: ... Complementary DNA (cDNA) was synthesized and amplified using a One Step RT PCR kit (Invitrogen) with PDLIM2-specific primers ...
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bioRxiv - Biochemistry 2020Quote: ... One aliquot was treated with DSG (disuccinimidyl glutarate) crosslinker (1 mM) (Thermo Scientific, Cat# 20593) and the other served as a DMSO only negative control ...
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bioRxiv - Microbiology 2020Quote: ... we used the RNA UltraSense™ One-Step Quantitative RT-PCR System from Applied Biosystems™ (11732927 ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were created using the Superscript IV One Step RT-PCR system (Thermofisher) and primers designed based on the results of Sanger Sequencing (Table 1) ...
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bioRxiv - Genomics 2020Quote: ... Purified dsRNA was quantified on a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and analysed by agarose/TAE gel electrophoresis to confirm quality and predicted size.
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bioRxiv - Genetics 2020Quote: ... genotyping was performed using one of four Affymetrix Axiom arrays (Affymetrix, Santa Clara, CA, USA) optimized for individuals of European ...
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bioRxiv - Microbiology 2021Quote: ... the ones with gene deletion were selected by PCR (DreamTaq DNA polymerase, Thermo Fisher Scientific) using the primers listed in supplementary material Table S3.
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bioRxiv - Microbiology 2021Quote: ... one milliliter of archaeal suspension transferred into a sterile Eppendorf tube (Fisher Scientific, Illkirch, France) was centrifuged at 17,000 g for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... One µg of isolated RNA was submitted to DNAse treatment (Ambion, Thermo Fisher Scientific Inc.) and then reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific Inc).
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bioRxiv - Microbiology 2020Quote: ... One µg of isolated RNA was submitted to DNAse treatment (Ambion, Thermo Fisher Scientific Inc.) and then reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific Inc).
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bioRxiv - Cell Biology 2021Quote: ... in a Step One Plus Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15 ...
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bioRxiv - Cell Biology 2020Quote: ... 1μl sample was used for one quantitative PCR reaction using 2X Cyber green mix (Thermofisher) and CFX96 (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl and one tablet of protease and phosphatase inhibitors (A32959, Thermo Fisher Scientific) for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... and pellets of one million cells were then lysed in 500 μl of TRIzol (Invitrogen) for subsequent RNA extraction.
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bioRxiv - Bioengineering 2022Quote: ... The concentration of the isolated RNA was quantified using NanoDrop One Spectrophotometer (Thermo Fisher Scientific), then complementary DNA was synthesized using a commercial kit (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... and qPCR was performed on AB Step One Plus real-time PCR machine (Applied Biosystems) using Fast SYBR Green Master Mix (Applied Biosystems).
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated using the SuperScript III one-step RT-PCR (#12574018, ThermoFisher Scientific, USA) and random hexamers (#48190011 ...
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bioRxiv - Physiology 2022Quote: ... A one-way analysis of variance (ANOVA) was performed with the Transcriptome Analysis Suite (ThermoFisher) on all samples ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA concentrations and purity were determined using a NanoDrop One (ThermoFisher Scientific, Watham, MA) spectrophotometer ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of the purified protein was determined using a Nanodrop One (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Donald Gullberg) for one hour and with the secondary Alexa Four 488 antibody (Life Technologies) for 30 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Transformations were performed in Top10 competent bacteria (One Shot TOP10 chemically competent E. coli, Invitrogen) and selected using 100 µg/mL ampicillin or/and 34 µg/mL chloramphenicol (Euromedex) ...
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bioRxiv - Genetics 2022Quote: ... Animals were then pelleted by centrifugation (2000 rpm for one minute) and Trizol reagent (Ambion) was added to maintain RNA integrity before storage at -80°C.
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed one time with DPBS (Fisher Scientific, product number 14-190-250) and fresh complete puromycin media was added to the cells for imaging.
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bioRxiv - Neuroscience 2021Quote: ... 60 degrees for 1 min] x 40 cycles) on a Step-One Plus (Applied Biosystems) using Sybr Green (Bio-rad ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with step One Real time PCR system (Applied Biosystems, Thermofisher Scientific). The qRT-PCR reaction systems were as follows ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with step One Real time PCR system (Applied Biosystems, Thermofisher Scientific). The qRT-PCR reaction systems were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was performed with a Step-One-Plus PCR system (Applied Biosystems) by the ΔΔCT method ...
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bioRxiv - Molecular Biology 2020Quote: ... and cloned into One Shot TOP10 cells with TOPO-TA cloning kit (Invitrogen, Waltham, MA). Cloned plasmids were screened for the ∼1150 bp insert using 100 pM of M13F (5’ GTAAAACGACGGCCAG ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using the SuperScript III One-Step RT-PCR kit (ThermoFisher Scientific) on a QuantStudio 7 Flex System (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... One microgram of total RNA was reverse transcribed using SuperScript VILO cDNA Synthesis kit (Invitrogen). PCR amplification was performed with the LC FastStart DNA master SYBR green I kit in a LightCycler 1.2 instrument (Roche Diagnostic ...
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bioRxiv - Microbiology 2021Quote: ... and qPCR was performed on a Step-One-Plus real time PCR machine (Applied Biosystems) using the StepOne Software v2.3 ...
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bioRxiv - Biophysics 2021Quote: ... The antibodies’ degree of labelling was measured by spectrometric analysis (NanoDrop One, Thermo Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... Proteins were buffer-exchanged using one or two consecutive Zeba spin desalting columns (Thermo Scientific) into 300 mM ammonium acetate ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with AgPath-ID One-Step RT-PCR reagents (Thermo Fisher Scientific), panRusV-2 primers (final concentration ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using the SuperScript III One-Step RT-PCR reverse transcription kit (Thermo Fisher Scientific, Poland). Species identification was first confirmed through PCR amplification and sequencing of Cytochrome B gene (Cytb) ...
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bioRxiv - Genomics 2022Quote: ... protein A/G magnetic beads (50 µl for one gram of tissue; Thermo Fisher Scientific) were added to sheared chromatin and incubated at 4°C for 1 hour with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... After washing the membrane was detected with One Step Ultra TMB-blotting Solution (Thermo Fisher) or SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).