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Citations for Thermo Fisher :
3001 - 3050 of 5129 citations for TAGAP Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... peptides were resuspended in MS-grade water and labeled with tandem mass tags (TMT 10-plex; Thermo Fisher). Samples were resolved by nano-electrospray ionization on an Orbitrap Fusion Lumos MS instrument (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75µm x 500 mm, 2µm particles; Thermo Scientific) at a flow rate of 300 nL/min (60° C ...
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bioRxiv - Cell Biology 2020Quote: ... The digests (2µL, corresponding to 2.5µg of peptides) were loaded onto a spectrometer (Q-Exactive Quadrupole-Orbitrap, Thermo Scientific) on a one-hour gradient with an intersample 30-minute blank loaded.
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bioRxiv - Microbiology 2021Quote: ... The peptide concentration was estimated using a BCA protein assay reagent kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... The peptides were separated on a Thermo EASY-Spray column (Thermo Scientific 50cm column, column temperature 45 °C) operated at a maximum pressure of 800 bar ...
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bioRxiv - Microbiology 2020Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Tryptic peptides (2μg) were loaded onto a C18 trap (75μm x 2cm; Acclaim PepMap 100, P/N 164946; ThermoFisher) at a flow rate of 2μL/min of solvent A (0.1% formic acid in LC-MS grade H2O) ...
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bioRxiv - Molecular Biology 2021Quote: ... The antiserum was affinity-purified using the peptide antigen coupled to UltraLink™ Iodoacetyl gel (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: The peptides were analyzed by nLC-MS/MS using an Ultimate 3000 RSLC nanosystem (Dionex, Thermo Scientific, USA) coupled to a Q Exactive mass spectrometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were separated across a 2.1 m□×□5 cm C18 separating column (1.9 mm Hypersil Gold, Thermo Fisher) with a linear gradient of 4-40% CH3CN and 0.3% formic acid over 6 min ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were subsequently on-line eluted into a Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) from an Acclaim PepMap™ RSLC C18 nano Viper analytical column (2 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were digested overnight (16 h) at 37°C and peptides were dried using a Speedvac (Thermo Scientific). Peptides were desalted with stage tips using the following procedure ...
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Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteinsbioRxiv - Biochemistry 2022Quote: Peptides were reconstituted in 10 μl of H2O and reacted with 80 μg of TMT10plex (Thermo Scientific, #90111) (Werner et al ...
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bioRxiv - Cancer Biology 2022Quote: ... 250 μg from each sample were digested into peptides with immobilised TPCK-trypsin beads (Thermo Fisher Scientific #20230) at 37°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Mass spectrometry analysis of tryptic peptides was performed on an LTQ-Orbitrap Elite (Thermo Scientific, San Jose, CA) mass spectrometer interfaced with an Easy-nLC 1000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting peptides were separated by nano-liquid chromatography on an easy-nLC 1200 system (Thermo Fisher Scientific) and directly sprayed into a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptides were separated by RPLC (50 cm x 75 μm Acclaim PepMap C18 column, Thermo Fisher Scientific) with over an 87-min gradient of ACN (4% to 25% ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were separated on a 50 cm and 75 μm inner diameter Easyspray column (ES803a, Thermo Fisher Scientific). Peptides were separated using a 190 min gradient of 6 – 27% acetonitrile in 1.0% formic acid with a flow rate of 300 nL/min ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptide concentrations were determined with the PierceTM BCA Protein Assay Kit according to the manufacturer’s instructions (#23225, ThermoFisher). Samples were measured on an Orbitrap Q-Exactive plus (Thermo ScientificTM ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted with 80 µg of TMT10plex (Thermo Scientific, #90111)22 label reagent dissolved in 4 µl of acetonitrile for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of peptide in stock solutions was determined using a NanoDrop One UV/Vis system (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 mins organic gradient ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted peptides were sprayed into a LTQ Orbitrap Velos mass spectrometer (ThermoFisher Scientific, San Jose, CA, USA) equipped with a nano-ESI ion source ...
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bioRxiv - Immunology 2022Quote: ... we determined the amount of peptide in the combined sample by Qubit™ Fluorometric Quantitation (Thermo Fisher Scientific). A 1 µg aliquot of each fraction was subjected to 1D-nano LC ESI-MS/MS (Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric ...
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bioRxiv - Developmental Biology 2022Quote: Desalted peptides were injected in an EASY-SprayTM PepMapTM RSLC C18 50cm X 75cm ID column (Thermo Scientific) connected to an Orbitrap FusionTM TribridTM (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: Peptides destined for phosphoproteome analysis were subjected to IMAC enrichment using a ProPacTM IMAC-10 column (Thermo Fisher) on a 1260 Infinity II system (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... The peptides from the cell line were analyzed by LC-MS/MS using the Q-Exactive (Thermo Scientific) and TripleTOF 5600 (AB Sciex ...
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bioRxiv - Molecular Biology 2023Quote: ... Mass spectrometric analysis of eluting peptides was conducted on an Orbitrap Exploris 480 (Thermo Fisher Scientific; Waltham, US) instrument platform ...
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bioRxiv - Neuroscience 2023Quote: Mass spectrometer for analysis of TMT-labeled peptides (Q-Exactive™ Plus Hybrid Quadrupole Orbitrap™, Thermo Scientific)
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bioRxiv - Neuroscience 2022Quote: ... The eluted peptides were analyzed on a quadrupole orbitrap Ion trap tribrid mass spectrometer (Fusion, Thermo Fisher Scientific) in data-dependent acquisition (DDA) ...
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bioRxiv - Molecular Biology 2022Quote: ... The desalted peptides were separated by EASY-nLC 1200 and analyzed by Orbitrap Eclipse mass spectrometer (Thermo Fisher) using sceHCD-MS2 and CID–MS2–MS3–EThcD–MS2 fragmentation approach essentially as described earlier37–39 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/2 peptide were separated and analyzed with a Nano-HPLC (EASY-nLC1200, Thermo Fisher Scientific, Inc., USA) coupled to Q-Exactive MS (Thermo-Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... eluted peptides were directly sprayed onto the Q Exactive HF Orbitrap LC-MS/MS system (Thermo Fisher Scientific), using the nanoelectrospray interface ...
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bioRxiv - Molecular Biology 2022Quote: ... Tryptic peptides were loaded onto a trap column (Pepmap 100, 5 μM, 5 x 0.3 mm, ThermoFisher Scientific) at a flow rate of 10 uL/min using 2% ACN and 0.05% TFA as loading buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... desalted peptides were injected into a Thermo LTQ Orbitrap XL Mass Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) or an Orbitrap Elite™ Hybrid Ion Trap mass spectrometer ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 0.6 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, ThermoFisher Scientific) and separated on a PepMap RSLC C18 column (50 cm × 75 μm ...
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bioRxiv - Systems Biology 2024Quote: ... peptide mixtures were separated by a reversed-phase chromatography (Dionex Ultimate 3000 NCS-3500RS Nano, Thermo Fisher Scientific) coupled on-line to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... but peptides were gradient eluted from the column directly to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher) using a 155 min acetonitrile gradient from 5 to 35 % B in 120 min followed by a ramp to 45% B in 10 min and 100% B in another 10 min with a hold at 100% B for 10 min (A=2% acetonitrile in 0.5% acetic acid ...
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bioRxiv - Cancer Biology 2024Quote: Peptide samples from a 24-hour experiment were injected on a Dionex Ultimate 3000 RSLC (Thermo Fisher Scientific) connected to an Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... 100 μg peptide mixture for each sample was labeled using TMT reagent according to the instructions (Thermo Scientific). The labeled peptides were separated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane permeability of the peptide was determined by using the LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen). S ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated Pol II CTD mimic peptide (20 µg) were immobilised on 10 µl streptavidin agarose resin (Thermo Scientific) for 2 hours at 4 °C in binding buffer (10 mM HEPES [PAA catalogue no ...
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bioRxiv - Microbiology 2024Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides generated were analyzed by electrospray ionization on an Elite tandem orbitrap mass spectrometer (Thermo Scientific Inc). Nanoflow HPLC was performed by using a Waters NanoAcquity HPLC system (Waters Corporation) ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were ionized using a nanospray ion source into a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). MS/MS spectra were searched against the Uniprot human complete proteome FASTA database downloaded on 2018_10_26 ...
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bioRxiv - Genomics 2024Quote: ... around 1 μg of total peptides were analyzed on an EASY-nLC 1000 UHPLC system (Thermo Fisher Scientific) coupled via a 20 cm C18 column ID picofrit column (New Objective ...
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bioRxiv - Neuroscience 2024Quote: Tryptic peptides were analysed by LC-MS/MS using an EASY-nLC system (Proxeon Biosystems, Thermo Fisher Scientific) connected to a Velos-Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: A vector containing the Streptavidin-Binding Peptide (SBP)-Tag was prepared in pEF1 (Thermo Fisher, Cat# V 92120), and SERBP1 ORF was cloned in frame with the His-Myc-tag present in the vector to create pSBP-SERBP1 ...
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bioRxiv - Molecular Biology 2024Quote: 800 ng of peptides were analyzed on a Q-Exactive Plus mass spectrometer (Thermo Scientific, San Jose, CA) coupled to an EASY-nLCTM 1000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 90% acetonitrile in water) was employed for peptide separation on an ASY-nLC 1200 UPLC system (ThermoFisher Scientific). The separation gradient was set as follows ...