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Citations for Thermo Fisher :
3001 - 3050 of 7890 citations for Recombinant Human NEK7 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Peaks enriched in neural crest samples were cloned from genomic DNA using standard PCR methods using AccuPrime Hi-Fidelity Taq polymerase (Invitrogen). Primers used to amplify target enhancer sequences are provided in the Key Resources table (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... respectively) were amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Fragments of genomic DNA upstream of the first methionine codon (2677 bp ...
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bioRxiv - Microbiology 2023Quote: ... All host defense systems identified in the various screens were placed under the araBAD promoter (arabinose inducible) in the pBAD Myc/His A vector (Invitrogen). Conjugative allelic exchange was carried out using R6K-origin pKEK2201 (kanamycin ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
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bioRxiv - Developmental Biology 2024Quote: ... and DsRed were cloned into pcDNA6 vector between the XhoI and XbaI sites (pcDNA6/Myc-His B; Invitrogen, Waltham, MA) with a poly(A)-tail of 84 nucleotides (Wakai et al. ...
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bioRxiv - Biochemistry 2024Quote: ... His-Smt3-Cysless GG-FAT10 (FAT10C0) was gene synthesized and subcloned into a pET28 vector by GeneArt (Thermo Fisher Scientific), the mutations are C7T ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted reaction was then transferred to a tube containing 80 µg of pre-washed Dynabeads His-Tag Isolation and Pulldown magnetic beads (Invitrogen) and incubated on a roller for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: The human anti-DNP and anti-WTA GlcNAc-β-4497 monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (21) ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Other monoclonal human anti-DNP and anti-WTA IgG of the four IgG subclasses were produced recombinantly in human Expi293F cells (Life Technologies) as described before 51 with minor changes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell binding experiment was done with the human T lymphocyte cell Jurkat and anti-human IgG antibody-Ax488 (ThermoFisher Scientific). Ax488 signal changes upon binding were detected using a flow cytometer (BD Accuri C6 ...
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bioRxiv - Cell Biology 2023Quote: HCT116 human colorectal cancer and SUIT-2 human pancreatic ductal adenocarcinoma cells were grown in McCoy’s 5A medium (Gibco, Cat. # 26600080) supplemented with 10% v/v dialyzed Foetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Human serum used for all conditions was from the same lot of pooled human AB serum (Fisher Scientific, Cat#: BP2525-100). For heat inactivation ...
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bioRxiv - Cell Biology 2024Quote: HepG2 (Human hepatoma cell line, ATCC, Manassas, Virginia, United States of America) and HEK293A (Human embryonic kidney cell line, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel droplets were overlaid with recombinant organoid medium: Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...