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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Recombinant Goat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Sections were subsequently incubated by primary antibody 1:500 (GFP recombinant rabbit monoclonal antibody; G10362, Life Technologies) for 48 hours at 4°C followed by three PBS washes ...
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bioRxiv - Microbiology 2022Quote: ... and 50 seconds elongation at 72°C with 1U of Pfu recombinant DNA polymerase (Thermo Fisher Scientific). The amplicon ORF45HA was cut with NheI/SmaI and inserted in pEGFP-C1 (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were generated in Sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen, USA). HEK293F cells were cultured under 5% CO2 at 37 °C and were used for transfection at a density of 2.5 × 106 cells/ml ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) and carried out in a total volume of 25 µL containing the following ...
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bioRxiv - Microbiology 2023Quote: ... 15μL of annealed guide RNA oligos were mixed with 15μL of 1μM recombinant Cas9 (IDT) in 220μL of Opti-MEM media (Gibco) and incubated for 5min at room temperature ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for recombinant DNA cloning and Golden Gate Assembly were purchased from Thermo Scientific (Waltham, MA) and New England Biolabs (Ipswich ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... either the recombinant CT584 or purified Cm EB’S were solubilized in SDS sample buffer (Novex, Life Technologies) in the presence of reducing agent (10mM DTT) ...
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bioRxiv - Plant Biology 2019Quote: ... Protein concentrations were determined using Pierce 660 nm protein assay (Thermo Scientific). Extracts with 500 μg of protein were alkylated with 14 mM chloroacetamide (CAA ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration was measured by EZQ protein quantification kit (Thermo Fisher Scientific), and 30 µg of protein from each sample was reduced with 10 mM dithiothreitol overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured by BCA Protein assay kit (Thermo Fisher Scientific) at 562 nm using BSA to produce a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) and purity was checked by sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined by a BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: Protein concentration was determined using a BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The amount of protein was determined using QuBit Protein Assay Kit (ThermoFisher) following the manufacturer’s instruction and 80 ug of protein was loaded per well ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A beads (Dynabeads Protein A, ThermoFisher Scientific, Waltham, MA, Cat #10002D) were added and samples were incubated at 4°C for 4 hrs ...
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bioRxiv - Genetics 2020Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Protein immunoprecipitation was performed using Dynabeads™ Protein G for Immunoprecipitation (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D). 30 µL of beads were used per IP ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by the BCA protein assay kit (Thermo Fisher). The protein solution was aliquoted into a small volume ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific). The lysates were fractionated with SDS– PAGE ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific). Then ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined with BCA protein assay (Thermo Fisher Scientific, 23225), and equal protein amounts were used in denaturing gel electrophoresis.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher). SDS-PAGE was used to measure beta glucocerebrosidase (GCase ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was measured using Coomassie Plus Protein Assay Reagent (Thermo scientific) and equal amounts of protein were loaded onto 6% SDS gels for anti-V5 and anti-TIM blots or 8% SDS gels for anti-CLK blots respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate protein concentration was measured with BCA Protein Assay (Thermo Fisher 23252). Lysate was diluted to 4 mg/ml in Sup6-150 ...
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bioRxiv - Molecular Biology 2021Quote: Protein content was determined using the Pierce BCA protein assay (Thermo Fisher). 4-10 μg of total protein in Laemmli sample buffer was resolved by SDS-PAGE on 9% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were measured by QuBit Protein Assay Kit (Thermo Fisher, Q33211) and sarkosyl buffer (50mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was measured with Qubit Protein Assay Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations were determined using BCA Protein assay Kit (Thermo Scientific Pierce). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... separating proteins on NuPage™ 4-12% Bis-Tris Protein Gels (Invitrogen) followed by transfer onto nitrocellulose or PVDF (for IR-Dye detection ...
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bioRxiv - Cell Biology 2022Quote: Solubilized sperm proteins were quantified using EZQ Protein Quantitation Kit (Thermo Fisher) and 2.25ug alkylated (Pierce ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... Protein concentration was determined using the BCA protein assay kit (ThermoFisher, #23225). 20 to 30 μg of protein extract was separated on 4-12% Bis-Tris plus gels ...
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bioRxiv - Cell Biology 2022Quote: ... and their protein levels measured with Pierce BCA Protein Assay kit (Thermofisher). EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was quantified using Pierce BCA protein assay kit (Thermo Fisher). Equal amount of protein were mixed with 5X SDS sample buffer [5%(vol/vol ...
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bioRxiv - Genomics 2020Quote: ... A mixture of magnetic Protein A and Protein G Dynabeads (Life Technologies) were used to isolate Ab:chromatin complexes ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration was measured using Pierce BCA Protein Assay Kit (Thermo Fisher). Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were quantified using Micro BCATM Protein assay kit (ThermoFisher Scientific). The cell lysates containing equal amounts of total proteins (30 μg ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was calculated using Pierce BCA protein assay kit (Thermo Fisher). 30 µg of protein was loaded on a 10% SDS-PAGE gel and separated by electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... Total cellular protein concentration was quantified via BCA Protein Assay Kit (ThermoFisher). 10 µg protein per sample was separated via 8% SDS polyacrylamide gel and transferred to PVDF membrane (ThermoFisher) ...
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bioRxiv - Neuroscience 2019Quote: ... and protein concentrations were determined using the BCA protein assay (Thermo Fisher). Biotinylated proteins were isolated by batch streptavidin chromatography ...
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bioRxiv - Immunology 2020Quote: ... Protein concentrations were determined using the Coomassie (Bradford) Protein Assay Kit (ThermoFisher) as directed ...
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bioRxiv - Physiology 2020Quote: ... Protein biomass was determined using a BCA Protein Assay Kit (ThermoFisher, MA). Previously frozen larval samples were removed from −80°C storage and resuspended in 500 μl of NANOpure (Barnstead ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations were estimated using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). Forty μg of total protein were separated in Bolt 4-12% Bis-Tris Plus Gels (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein quantification was performed with BCA Protein Assay Kit (Thermo Scientific-Pierce). After SDS/PAGE gel electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: ... The protein concentration was measured with Qubit Protein Assay Kit (Invitrogen, Q33211) and Qubit 4 fluorometer ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Pierce BCA Protein Assay kit (Thermo Scientific) and 50 µg of each protein sample were resolved onto 17.5% SDS-PAGE gels ...