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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: Human islets were cultured in CMRL medium (Gibco 11530-037) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 ng/mL recombinant human EGF (Gibco Cat# PHG0311).(22 ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs were purchased from Life Technologies (for human cell lines) and from Horizon Discovery (for human PCLS) ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human EGF (Thermo Fisher Scientific, #PHG0311, 50 ng/ml), N-acetylcysteine (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...
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bioRxiv - Neuroscience 2021Quote: ... The presence of secreted Fc Fusion protein in the medium was confirmed by immunoblotting with goat anti-human IgG antibody (Invitrogen, A-21433, 1:500).
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with 150 μL 1% BSA/PBS and then incubated with 50 μL Goat anti-Human IgG Fc secondary antibody PE (ThermoFisher, cat #12-4998-82) at a final concentration of 2.5 μg/mL in 1% BSA/PBS ...
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bioRxiv - Immunology 2023Quote: Cells were stained for viability (Supplementary table 1 and Supplementary table 4) and blocked for CD16/32 with anti-human FC Receptor binding inhibitor (ThermoFisher Scientific, 14-9161-73) in PBS for 15 minutes at room temperature (Spn ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Microbiology 2023Quote: ... A portion of the Fc blocked-cells were separated to accommodate the unlabeled control while remaining samples were incubated with mouse anti-human HLA-ABC W6/32 (Invitrogen [MA5-11723], 1:40) on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS at room temperature in the dark and incubated in the appropriate secondary antibody (Anti-human 488 and Anti-rabbit 647, Invitrogen, both at 1:500) at room temperature for 1 hour while on a rotary shaker (65 rpm) ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were washed with media twice and labeled using a mouse primary antibody targeting human ICAM-1 (Thermo Fisher Scientific, Cat. # 14-0549-82) at an appropriate working concentration (1:100 dilution in media ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Complement activation was measured by quantifying deposited C3b upon adding the wells with mouse monoclonal anti-human C3b antibody (MA1-82814, ThermoFisher Scientific, diluted 1:1,000) diluted in PBS 1X/BSA 1% and incubating 1 h at ambient temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Bound ch5D3 was detected by goat anti-human secondary antibody conjugated to Alexa Fluor 647 (1 µg/mL; Thermo Fisher Scientific; cat.no. A-21445). All incubations were carried out 15 minutes at 4°C followed by stringent washes ...
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bioRxiv - Microbiology 2021Quote: ... which was boiled in the SDS loading buffer with 5% β-mercaptoethanol (Cat. #60-24-2, Acros Organics). The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using TaqMan Gene Expression Assay for rat β-actin (Actb, Rn00667869_m1, Applied Biosystems), rat Taste receptor type 1 member 3 (Tas1r3 ...
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bioRxiv - Bioengineering 2019Quote: ... and cells were washed once with DPBS and incubated with β-Galactosidase Assay Reagent (75705, Thermo Fisher Scientific), for 30 minutes at 37°C ...
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bioRxiv - Genetics 2020Quote: ... Then 5 × 106 cells were used to perform β galactosidase activity using Tropix Gal-Screen System (Applied Biosystems). The readings were normalized to MTG16 wild type activity ...
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bioRxiv - Biochemistry 2021Quote: ... samples were prepared after addition of 2.5 % β-mercaptoethanol to sample buffer (NuPAGE LDS sample Buffer, Life Technologies) and boiling for 5 min at 90 °C ...
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bioRxiv - Cell Biology 2021Quote: The following monoclonal antibodies were used: mouse anti-β-ACTIN clone BA3R (cat# MA5-15739, Thermo Fisher Scientific), mouse anti-XBP-1S clone E7M5C (cat#47134S ...
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bioRxiv - Molecular Biology 2022Quote: ... 90 µM β-mercaptoethanol (MilliporeSigma, Cat# M3148) and 100 U/mL penicillin-streptomycin (Thermo Fisher Scientific, Cat# 15140122). INS-1E cells were cultured in 11 mM RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... The heads were then homogenized in loading buffer (4X Bolt LDS, Invitrogen, Novex with 5% β-mercaptoethanol added) and heated for 10 min ...
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bioRxiv - Microbiology 2021Quote: Cell free glycosylation was conducted in S30 buffer with 0.1% n-dodecyl-β-d-maltopyranoside (DDM; Thermo Scientific) and 10 mM MnCl2 (Across Organics) ...
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bioRxiv - Plant Biology 2021Quote: ... which contained a β-glucuronidase (GUS) gene fusion created via LR Clonase (Thermo Fisher Scientific, Waltham, MA, USA) reactions.
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bioRxiv - Genetics 2022Quote: ... can be measured in live cells by using 5-(pentafluorobenzoylamino) fluorescein Di-β-D-glucopyranoside (PFB-FDGlu; ThermoFisher) as a substrate that is taken up into acidic vesicles only ...
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bioRxiv - Bioengineering 2019Quote: ... β-galactosidase enzymatic activity in tissue lysates was measured using the Galacto-Star System (Applied BioSystems; Bedford, MA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM MgCl2) with 1.5% n-Dodecyl β-D-maltoside (DDM) and 1X Halt Protease Inhibitor (Thermo Scientific) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2020Quote: 58α−β− T cell hybridoma and M12 B cell lymphoma cell lines were cultured in RPMI 1640 (Gibco) supplemented with 5% FBS (Atlanta Biologicals) ...
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bioRxiv - Biochemistry 2020Quote: ... a β-lactamase (TEM) reporter assay was performed using the LiveBLAzer-FRET B/G Loading Kit (Life Technologies). HEp-2 cells were seeded in 8-well μ-slides (Ibidi ...
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bioRxiv - Developmental Biology 2019Quote: ... 6 mM β-mercaptoethanol and 0.5% NP-40) supplemented with 75 units/ml RNAse inhibitor (Thermo Fisher Scientific) and 1X mammalian protease inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... single cell suspensions were mixed with 100 μL prewarmed 2 mM fluorescein di-β-D-galactopyranoside (FDG) (Invitrogen) diluted in water and then incubated in a 37°C water bath for 1-2 minutes to permit substrate uptake ...
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bioRxiv - Cancer Biology 2022Quote: ... TGF-β levels (pg/mL) were determined using a commercial murine ELISA kit (#BMA608-4; Invitrogen, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and combined with 11 μL loading buffer (4X NuPAGE LDS sample buffer, Thermo Fisher, containing 10% β-mercaptoethanol). The sample was heated at 95°C for 5 min and loaded into a 4-12% Criterion XT Bis-Tris Protein Gel ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 50 μmol/L of β- mercaptoethanol and 100 units/mL of Penicillin/Streptomycin (Thermo Fisher Scientific). Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl-β-D-thiogalactopyranoside (IPTG) and 3-(N-morpholino) propane sulfonic acid (MOPS) were purchased from Fisher Scientific. MTSES was purchased from Biotium.
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bioRxiv - Neuroscience 2023Quote: ... cells were first incubated with a rabbit anti-mouse C/EBP β primary antibody (Invitrogen, Catalog #PA5-104823) which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ ...
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bioRxiv - Developmental Biology 2023Quote: To assess the levels of β-galactosidase activity in senescent cells a CellEventTM Senescence Green Detection Kit (Invitrogen) was used ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
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bioRxiv - Neuroscience 2024Quote: ... TaqMan assay IDs were Mm00802131_m1 for Aqp4 and Mm00607939_s1 for β-actin (Thermo Fisher Scientific, Waltham, MA, USA), whereas primer sequences for M1 and M23 isoforms were synthesised according to (Fallier-Becker et al. ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... 24 aliquots of human blood (20 μL) from each donor were pipetted into 1 mL 2D-barcoded tubes (Matrix Storage Tubes; Thermo Fisher Scientific Inc., Waltham, MA), placed into a ANSI/SLAS microplate format compatible 96-tube racks (8 × 12 ...