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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and transferred to Nitrocellulose or PVDF membranes (Invitrogen) using the iBlot 2 Transfer System (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to nitrocellulose membranes (IB301031, Invitrogen) using iBlot (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... and transferred to a PVDF Membrane (Invitrogen, IB24002) using an iBlot2 device ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred onto PDVF membrane (Thermo Scientific) for 1.5 hours at 300 mA on ice ...
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bioRxiv - Biophysics 2024Quote: ... Denmark).Macrophage membrane cholesterol was detected by Invitrogen Amplex-Red assay kit (Catalog number ...
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bioRxiv - Biochemistry 2024Quote: ... transferred to nitrocellulose membranes (iBlot, Thermo Fisher Scientific), blocked with a 1X TBST buffer supplemented with 5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... on Basement Membrane Matrix (Fisher Scientific, CB-40234A)-coated 24-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... NC membranes were imaged on iBright 1500 (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... NC membranes were imaged on iBright 1500 (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... then transferred onto a PVDF membrane (Invitrogen, IB24001) using iBlot 2 semi-dry transfer system (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... A20004)] or membrane staining with Bodipy TR ceramide (ThermoFisher D7540 ...
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bioRxiv - Biochemistry 2024Quote: ... NC membranes were imaged on iBright 1500 (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to a PVDF membrane (Thermo Fisher). Membranes were incubated overnight with 1:1000 anti-CDKN1B (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to PVDF membranes (Thermo Scientific, PI88520) after electrophoresis ...
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bioRxiv - Developmental Biology 2024Quote: ... Reduced Growth Factor Basement Membrane Matrix (Life Technologies). Cells were passaged using gentle dissociation with 0.5 mM EDTA in PBS.
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bioRxiv - Developmental Biology 2024Quote: ... The transfer onto a PVDF membrane (IB24001, Invitrogen) was done using the iBlot 2 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... The full-thickness skin tissues from the cell injection spots were collected and placed in an Optimal Cutting Temperature compound (Thermo Fisher Scientific, Hampton, NH, USA) on dry ice for embedding and freezing ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal brain slices containing anterior and posterior sections of the SC were prepared at 300 μm thickness in ice-cold HBSS (Gibco/Thermo Fisher Scientific, Waltham, MA) on a vibratome (Leica VT1000S ...
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bioRxiv - Biophysics 2020Quote: ... During the same time period the cell membrane is stained using a plasma membrane dye (CellMask™ Deep Red, ThermoFisher Scientific) at 0.5 μM concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins in the gel were then transferred to a membrane using the iBlot 2 membrane transfer device (Invitrogen, IB21001, US) (2014) ...
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bioRxiv - Microbiology 2022Quote: ... To stain the plasma membrane of hemocytes we used CellMask green plasma membrane stain stock solution (C37608, Invitrogen, Waltham, MA, USA) and for the nuclei we used the Hoechst 33342 Solution (20mM ...
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bioRxiv - Cell Biology 2022Quote: For extraction of membrane and cytosol proteins the cells were lysed according to the Mem-PER Plus Membrane Protein Kit protocol (#89842, ThermoFisher Scientific). The supernatants were kept frozen in −80 °C until total protein quantification ...
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bioRxiv - Neuroscience 2023Quote: ... were subjected to SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes or nitrocellulose membranes and blocked with I-Block (ThermoFisher, T2015). Primary antibodies used were ...
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bioRxiv - Cell Biology 2024Quote: ... the parasite suspension prepared as described above was additionally filtered through a hydrophilic membrane of 5 μm pore size (Cyclepore Tracked Etched Membrane, Cytiva, Fisher Scientific). The functionalized 18- or 32 mm diameter glass coverslips (micropatterned or not ...
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived NGN2 neurons were maintained in 6-well plates and were lysed on day 21-24 for membrane protein extraction using the Mem-PER Plus Membrane Protein Extraction kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... transferred to nitrocellulose membranes and the membranes were probed with the following antibodies: a rabbit anti-MAVS polyclonal antibody (Thermo Fisher), a rabbit anti-STING polyclonal antibody (Cell Signaling) ...
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bioRxiv - Microbiology 2023Quote: An amount of 2 mL of the above diluted culture (∼5×106 cells/species/membrane) was then spread evenly and immobilized on a 0.2 μm nitrocellulose filter membrane (4.7 cm diameter; Thermo Fisher Scientific, MA) on a flat-bottom ...
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bioRxiv - Plant Biology 2022Quote: ... Elution and separation of peptides on the analytical column (75 cm x 75 µm analytical EASY-Spray column packed with PepMap RSLC C18, 2 µm material, Thermo Fisher Scientific; heated to 60 °C) was achieved at a flow rate of 300 nl min-1 using a 140 min gradient going from 95 % buffer A (0.1 % formic acid ...
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bioRxiv - Microbiology 2024Quote: ... Elution and separation of peptides on the analytical column (75 cm x 75 µm EASY-Spray column packed with PepMap RSLC C18, 2 µm material, Thermo Fisher Scientific; heated to 60°C) was achieved at a flow rate of 300 nl min-1 using a 140 min gradient going from 95% buffer A (0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Both genomic DNA and cDNAs were analyzed on DT96 real-time DNA amplifier (DNA-Technology) using SYTO™ 13 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific). RT-qPCR values were normalized to the rp49 mRNA and genomic DNA qPCR values were normalized to rp49 genomic qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... all samples were purified using AmpureXP beads at a volumetric ratio of 0.5X with a final elution in 13 µl of H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The cDNA was then quantified using the Qubit High Sensitivity dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... As a control, siGENOME Non-Targeting Pool #1 (Dharmacon, D-001206-13-05) and a second independent predesigned PURA siRNA (Thermo Fisher Scientific, 289567) was used as described for the PURA siRNA pool ...
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bioRxiv - Plant Biology 2021Quote: ... was carried out to determine the relative expression levels of 13 selected genes on StepOne™ Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green ...
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bioRxiv - Microbiology 2022Quote: ... which were cut into small pieces of ∼5 × 5 mm and sterilised with 100% ethanol (for light microscopy and SEM) or 13 mm round Thermanox plastic coverslips (Nalgene Nunc International, Rochester, NY) scratched with sandpaper and sterilised with 100% ethanol (for SBFSEM ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was harvested from mouse islets and INS1(832/13) cells by using a QIAGEN RNeasy Plus Kit (Cat# 74034) and a TRIzol™ reagent (Invitrogen, cat#15596018) respectively ...
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bioRxiv - Neuroscience 2022Quote: ... were stained for hematoxylin and eosin or immunostained for glial fibrillary acidic protein (GFAP; rat-anti-GFAP; #13-0300 Thermo Fisher Scientific; RRID:AB_2532994), ionizing calcium binding adaptor protein 1 (IBA1 ...
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bioRxiv - Microbiology 2022Quote: ... cultures were diluted to an OD750 nm = 1.0 in 200 μL of the respective medium and monitored with multipoint absorbance measurements (13 coordinates, OD750 nm) over 20 min on a Varioskan Flash microplate reader (Thermo Fisher Scientific, Germany). Microplates were kept in the reader at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822], anti-IL-13 PE/Cy7 [Invitrogen, 25-7133-82]) at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells treated with the indicated concentrations of selected compounds for 72 h in 100 μL of incubation media were supplemented with 20 μL of 0.6 mM resazurin (Acros Organics 62758-13-8) and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC colonies were detached in clusters using a 13 min incubation with collagenase type IV (250 IU/mL final concentration) (Thermo Fisher Scientific; 17104019) and transferred to 6-well low adhesion plates in ESC media supplemented with 10 µM ROCK Inhibitor (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... I/1Ki (boa constrictor kidney cells)81 and I/1Ki-Δ (refered to as I/1Ki/SDeV in this paper)13 were maintained in Minimal Essential Medium Eagle (MEM; Gibco #31095-029) supplemented with 10% heat-inactivated fetal calf serum (Sigma-Aldrich #F75424) ...
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bioRxiv - Bioengineering 2023Quote: ... Donor blood (20 mL) was collected in a sterile fashion into BD vacutainer spray coated K2EDTA tubes (Fisher Scientific, cat. 13-680-61) and PBMCs were isolated using density gradient centrifugation on Histopaque and expanded by using StemPro-34 Serum Free Complete Media (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 25,000-50,000 cells were plated and transfection of specified constructs or empty vector (6-13 µg/nL) was performed with Lipofectamine 2000 (Thermo Fisher Scientific, 1166819) and Opti-MEM (Gipco ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of each sample was pooled together to make a “pooled sample” for a given derivatization batch and 80 μL of each individual sample were loaded into a 150 μL glass insert (CAS#13-622-207, Thermo scientific, Florence, KY) inside of a GC vial with a 9 mm PTFE/red rubber septum (CAS#C4000-30 ...
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bioRxiv - Microbiology 2023Quote: Axenic haptomonad cells were generated by culturing 1 × 106 cells/ml promastigotes on 13 mm round Thermanox plastic coverslips (Nalgene Nunc International, Rochester, NY) scratched with sandpaper and sterilised with 100% ethanol (for proteomics and electron microscopy ...
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bioRxiv - Genetics 2023Quote: ... amount of diluted Cas9 Nuclease (Cat#1081058) and gRNA:tracrRNA duplex were mixed with 13 µL of Opti-MEM Medium (Thermo Fisher Scientific, Cat#51985091) and incubated for 5 minutes at room temperature ...
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bioRxiv - Pathology 2024Quote: ... Oligos were resuspended into a 10 µM working stock and set up in a PCR reaction to prime to the CRISPR tail (primer 13) with dNTPs and high-fidelity polymerase (Phusion polymerase, Thermo Scientific #F-530S) (Ta = 55 °C ...