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Citations for Thermo Fisher :
2951 - 3000 of 5181 citations for S100A12 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... PCR products were then cloned into corresponding human IgG1 expression vectors with Gibson Assembly (Thermo Fisher Scientific) and the mixture was subsequently transformed into DH5α cells ...
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bioRxiv - Neuroscience 2024Quote: Elastase generated granulin peptides were obtained by incubation of rPGRN with human neutrophil elastase (# RP-77526, Invitrogen). 50 ng/µl PGRN and 80 ng/µl elastase were incubated in 100 mM sodium citrate pH 6.0 overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... 1:2000 dilution of secondary Alexa FLUOR-647 GOAT-Anti human IgG [H+L] (Invitrogen; #A-21445) was added to the cells for 30 minutes followed by 3 washes with FACS Buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Human iPSC spheroids were washed and growth factor-depleted using DMEM/F12 (Thermo Fisher Scientific #21331-046) for 2 h prior to stimulation ...
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bioRxiv - Systems Biology 2024Quote: Human osteosarcoma epithelial cells (U-2 OS cell line) were cultivated in DMEM high glucose medium (Gibco), supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary staining was done using a goat anti-Human Alexa Fluor (AF) 594 antibody (1:1000, Invitrogen), for 30 minutes ...
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bioRxiv - Pathology 2024Quote: Gene expression on human cells and tissue was quantified using TaqManTM assays (Fisher Scientific, Supplementary Table 3) and Luna® Universal qPCR Master Mix (M3003E ...
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bioRxiv - Pathology 2024Quote: ... and CXCL1 levels were measured in the supernatant of human precision-cut liver slices using ELISA (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Passage matched (±3) human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Life technologies #A3349401) on Matrigel (Corning ...
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bioRxiv - Systems Biology 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... Since most present-day data was generated using different SNP arrays (Axiom LAT1 and Human Origin Affymetrix), this data was phased and imputed by array using TOPMed Michigan Imputation Server ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... left in resting state or activated with Dynabeads Human T-Activator CD3/CD28 (1161D, Thermo Fisher Scientific) for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-Be(2c) and SK-N-SH human neuroblastoma cells were cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic lung fibroblasts (MRC-5, European Collection of Cell Cultures) were maintained in DMEM (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: ... All human primary cell lines were cultured in DMEM high glucose supplemented with GlutaMAX (Gibco, 10566-016), with further addition of 15% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... followed by PBS washing and immunostaining with secondary AlexaFluor633 anti-human antibodies (Thermo Fisher Scientific, #A-21091) for 30 mins at 4°C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: sEV isolated from human HTLV/T cell cultures with Total Exosome Isolation Reagent (#4478359, Thermo Fisher Scientific) were solubilized in SDS buffer ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney (HEK) 293T (#CRL-3216, ATCC) cells were maintained in DMEM GlutaMAX medium (#61965026, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (#15140122 ...
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bioRxiv - Biochemistry 2024Quote: ... human full-length VPS35L (untagged, synthesized as a codon-optimized GeneString from Thermo Fisher to improve expression), VPS26C (untagged) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). For comparing tumor cell growth in different groups ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA hybridized to a Mouse Clariom S Human Transcriptome Assay microarray plate (Affymetrix, Santa Clara, CA) and the plate was scanned by a GeneTitan MC Instrument (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Immunology 2023Quote: Immortalized human SZ95 sebocytes (Zouboulis et al., 1999) were cultured in T-75 culture flasks (Thermo Scientific) with Sebomed basal medium (DMEM/F12 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once and then incubated with Alexa Fluor 488 goat anti-human IgG antibody (ThermoFisher) diluted 1/200 in PBS + 1% FCS or BB buffer ...
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bioRxiv - Cell Biology 2023Quote: The release of CXCL10 to the culture medium was detected using Human ProcartaPlex immunoassays (Invitrogen, Vienna, Austria) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Human embryonic kidney 293T cells were grown in Complete DMEM (Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 8 µg of cDNA was fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Hepatic gene expression was measured using Affymetrix Human Exon 1.0 ST v1 Array (Affymetrix, Santa Clara, CA) in adolescents and Illumina Human-6 Expression Bead Chips (Illumina ...
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bioRxiv - Bioengineering 2022Quote: Intracellular mitochondrial presence within human heart organoids was visualized using Mitotracker Deep Red FM (Thermo Fisher Scientific). Mitotracker was prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Calcium transient activity within the human heart organoids was assessed using Fluo-4 AM (Thermo Fisher Scientific). Fluo-4 AM was solubilized in DMSO per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...
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bioRxiv - Biochemistry 2023Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA) ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... HCC1395 and SF295 human cancer cell lines were grown in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA) medium supplemented with 10% FBS and 50 units/ml of penicillin/streptomycin.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
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bioRxiv - Immunology 2023Quote: The human promyelocytic leukemia HL-60 cell line (ATCC #CCL-240) was maintained in IMDM media (Gibco) with 10% heat-inactivated (HI ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
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bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
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bioRxiv - Immunology 2023Quote: The human myelogenous leukemia cell line K562 was cultured in Roswell Park Memorial Institute (RPMI) media (Gibco) with 10% fetal bovine serum (FBS ...