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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for Recombinant Mouse Fetub His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 350 goat anti-mouse and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were obtained from Thermo Scientific. BHA (Cat# B1253 ...
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bioRxiv - Cell Biology 2023Quote: ... Alex Fluor 647 goat anti-mouse and Alexa Fluor 594 goat anti-mouse from Invitrogen. All secondary antibodies were used at a concentration of 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti 6xHis (mouse monoclonal, HIS.H8, Thermo Fisher Scientific, MA1-21315; WB 1:2000), anti GFP (mouse monoclonal ...
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bioRxiv - Genomics 2023Quote: ... EY.T434 mouse embryonic fibroblast cells mouse embryonic fibroblast cells were grown in DMEM (ThermoFisher 10566016) supplemented with 15% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa 594 goat anti-mouse/rat (1:1000, Invitrogen A-11032 mouse, A-11007 rat) and DAPI (1:500 ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Microbiology 2023Quote: ... The TMT10-plex-tagged sample (1 µg) was loaded onto an Acclaim PepMap100 C18 trap column (Thermo Fisher Scientific, 100 µm × 2 cm, 100 Å, 5 µm) at 5 µL/min using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... They were then rinsed in block for >30 min at room temperature before incubation in secondary antibody (1:250 Invitrogen #A21-42 AF488 anti-mouse IgM; 1:250 Life Technologies #A11029 AF488 anti-mouse IgG1; 1:250 Invitrogen #A21141 AF488 anti-mouse IgG2b) in blocking solution for > 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and secondary antibody (anti-mouse IgG2a AF647, 1:2000, Invitrogen, A-21241; anti-mouse IgG AF568, 1:2000, Invitrogen, A-11031; anti-mouse Cy5, 1:1000), sequentially ...
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bioRxiv - Biophysics 2020Quote: The pFAST-bac1vectors were used to produce recombinant baculovirus according to the protocol for the Bac-to-Bac system (Life Technologies). Recombinant GST-A3H Hap I WT and mutant baculovirus (K117E ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Recombinant bacmids were made according to instructions for the Bac-to-Bac® Baculovirus expression system given by the manufacturer (Invitrogen) using DH10EMBacY (Geneva Biotech) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were isolated according to standard protocols and recombinant plasmids were subjected to sequencing using universal M13 primers and the BigDye terminator cycle sequencing kit v1.1 (Thermo Fisher Scientific). Sequencing products were EDTA/ethanol-precipitated ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was used to infect NIH3T3 cells and stably expressing cells selected with 5 μg/ml puromycin (Thermo Fisher, A1113803). Conditional expression of CB1R was induced by the addition of Doxycycline (Millipore Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and further digested for 5 min at room temperature (22°C) using a TrypLE Express recombinant protease (ThermoFisher Scientific, cat. #12605010). The dissociated cells were sedimented by centrifugation (900 g for 10 min at 4°C) ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Developmental Biology 2019Quote: ... Recombinant HSF2 (TP310751 Origen) was desalted (ZebaTM Spin Desalting Columns, 7K molecular-weight cutoff, 0.5 ml (1034–1164, Fisher Scientific, Germany)) and biotinylated at a molar ratio biotin/protein (3:1 ...
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bioRxiv - Cell Biology 2019Quote: ... sequence-verified plasmids (wild-type and mutant variants of pbiG2ab:6His-ZZ-SNAPf-DHC1/IC2/LIC2/Tctex1/Robl1/LC8) were used to generate recombinant baculoviral genomes by Tn7 transposition into DH10Bac cells (Life Technologies). White ...
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bioRxiv - Developmental Biology 2019Quote: ... was introduced into the BglII and SpeI sites of pCR4-HBB-24XMS2SL and the recombinant HBB- 24XMS2SL-MCS sequence was subcloned into the pcDNA3 mammalian expression vector (ThermoFisher Scientific) using the NotI and XbaI sites ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculoviruses for expression of the mTTYH3 ECD1-T4L-ECD2 construct were generated using the Bac-to-Bac system (ThermoFisher Scientific). Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems) ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Immunology 2021Quote: CASP1-/- and NLRP1-/- N/TERT-1 keratinocyte cells were prepared by delivery of Cas9 ribonucleoprotein complexes containing an Alt-R CRISPR-Cas9 sgRNA and recombinant Cas9 (IDT) using the Neon Transfection System (ThermoFisher Scientific) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... luminescence was compared to a ladder of recombinant Gaussia Luciferase (NanoLight #321-500) quantified using a Gaussia Luciferase Glow Assay Kit (ThermoFisher #16161) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 150 ng were used for the BP recombinant reaction with pDONR221 according to the Gateway technology manual (Thermo Fisher Scientific). Chemically competent E ...
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bioRxiv - Cell Biology 2021Quote: ... two and three consecutive phage enrichment rounds were performed either with recombinant Miro1 immobilized on NuncTM ImmunoTM MaxiSorpTM tubes (Thermo Scientific) or HEK293-expressed GFP-Miro1 immobilized on ChromoTek GFP- Trap® Multiwell plate (Proteintech) ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Plant Biology 2021Quote: ... Inserts from the recombinant pENTR plasmids were transferred to destination vectors via gateway cloning by using the LR clonaseTM II Enzyme mix (Invitrogen, USA) and by following the manufactureŕs instructions ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant baculovirus was generated in Sf9 cells using the Bac-to-Bac system according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Recombinant proteins were then expressed by infecting Hi5 cells for 96 h at 28°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... or HIF1β were expressed in Sf9 cells that had been infected with recombinant baculovirus as described in the Bac-to-Bac® Baculovirus Expression System from Invitrogen.
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...