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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 59°C for 30s and 72°C for 30s using recombinant 5U/ul Taq polymerase prepared in 10X Buffer with 50mM MgCl2 (Invitrogen). The 388bp PCR amplicon was digested with 20U/µL EcoRV restriction enzyme prepared in 10X NEB 3.1 buffer (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Immunology 2022Quote: NK cells were enriched and incubated with 50 ng/ml recombinant mouse IL-15 (Preprotech) for four days in RPMI plus 10 % FBS with penicillin and streptomycin (Thermofisher) added in a U-bottom 96-well plate at the concentration of two million cells per ml ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids were transfected into Drosophila S2 cells using Calcium Phosphate Transfection Kit (Thermo Fisher Scientific, Paisley, Scotland, UK). Following antibiotic selection with Zeocin (InvivoGen ...
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bioRxiv - Microbiology 2020Quote: ... a recombinant bacmid DNA was generated from the sequencing-confirmed pFastBac construct using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... The bacmid was transfected into Sf9 cells to obtain recombinant baculovirus using Cellfectin II Reagent (Invitrogen Co., Carlsbad, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... fluorescent conjugate cholera toxin B (CTB; recombinant cholera toxin subunit B conjugated with Alexa fluorophores: 0.2% CTB-488 and CTB-594; Life Technologies) was injected into AL and PM.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
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bioRxiv - Microbiology 2020Quote: ... the macrophages were allowed to adhere to the culture plate for 20 minutes before 100 units (U) of murine recombinant interferon-gamma (IFNγ) (Invitrogen) was added to each well to stimulate the macrophages ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... These recombinant cells expressing individual rod opsin variants were then surface immunostained with a Dylight 550-labeled anti-HA antibody (ThermoFisher) and then fractionated according to the immunostaining intensity of expressed variants using FACS ...
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bioRxiv - Cancer Biology 2019Quote: ... The recombinant vector and miR-659 mimic were jointly transfected into MDA-MB-231 cells using Lipofectamine™2000 (Invitrogen). These cells were lysed after 24 h of culture and the activity of luciferase was determined.
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bioRxiv - Microbiology 2019Quote: ... Eight μL of Cellfectin II reagent and 3 ng of each recombinant plasmid were diluted in 100 μL Grace’s medium (Thermofisher Scientific), respectively ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Microbiology 2019Quote: ... Stable cell lines expressing the recombinant protein were initially maintained in complete medium comprising of serum free medium Sf-900 II SFM (ThermoFisher) complemented with 10% v/v foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Microbiology 2019Quote: ... bacteria resuspended to OD660 0.3 were incubated with 1 µg of recombinant protein in the presence of 1x protease inhibitors (Pierce, Thermo Scientific) in sterile 1.5 ml microcentrifuge tubes with gentle end-over-end mixing for 30 min at 25°C.
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then activated by the addition of 0.2 μg/ml LPS and 0.1 ng/ml murine recombinant interferon-γ (IFNγ) (both ThermoFisher Scientific) for 24 h ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... for 3 hr at 37 °C with 10 μg/mL recombinant laminin-521 (BioLamina, LN521-05) diluted in PBS+/+ (Gibco). Single-cell suspensions were then incubated for 3 hr at 37 °C in HUESM medium supplemented with 20 ng/mL bFGF and 10 μM Rock inhibitor Y27632 ...
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bioRxiv - Biochemistry 2020Quote: ... in a donor vector (pDONR-221) and transferred by recombinant cloning into the glutathione S-transferase (GST) fusion vector pDEST15 (Invitrogen). The plasmid was used to transform Escherichia coli ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Immunology 2022Quote: ... supplemented RPMI (RPMI-1640 supplemented with 10% fetal bovine serum, penicillin-streptomycin, Sodium Pyruvate, MEM NEAA, 30U/ml recombinant IL2 (ThermoFisher) and 55 µM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: Recombinant baculovirus was produced using sf9 insect cells following the instructions of Bac-to-Bac TOPO Expression System (Invitrogen A11339). Suspended HEK293S GnTI- cells at the concentration of 3.5-4.0×106/mL in 37°C were infected with P2 virus ...
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bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
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bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 488/555/647 Conjugate (CTB-488/555/647, 0.1% wt/vol, 80 nL, ThermoFisher C34775/C34776/C34778 ...
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bioRxiv - Molecular Biology 2023Quote: Introduction of recombinant genes into the parental HEKR4 cell line was performed according to the manufacturer’s guidelines (Thermo Fisher Scientific). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant pCS2+ plasmids containing the ORs were purified using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). The plasmids were linearized by NotI (Promega ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...