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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... an additional 100 μL of EGM-2 without growth factor was added with 1.5 μL of 1 mg/mL calcein-am (ThermoFisher Cat # C1430). Cells were then imaged 30 minutes later at the 4-hour timepoint ...
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bioRxiv - Neuroscience 2023Quote: ... and 62.5 ng of cDNA was amplified using TaqMan probes for vascular endothelial growth factor alpha (Vegfa) and 18S as endogenous control (Life Technologies), and SYBR green probes for cd93 ...
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bioRxiv - Cancer Biology 2023Quote: ... per condition with 200 μl serum-free DMEM supplemented with 20 ng/ml basic fibroblast growth factor (Invitrogen, Grand Island, NY), 10 □ng/ml epidermal growth factor (BioSource ...
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bioRxiv - Immunology 2022Quote: ... the eBioscience FoxP3/Transcription Factor Staining Kit (00-5523-00) was used per manufacturer’s instructions with intracellular antibodies: PE-conjugated RORγT (Invitrogen; 12-6981-82), PE-Cy7-conjugated FoxP3 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Cell Biology 2023Quote: ... 30,000 fibroblasts/mesenchymal cells per well) in modified MTEC medium diluted 1:1 in growth factor-reduced Matrigel (cat. no. CB-40230A, Thermo Fisher). Modified MTEC culture medium is composed of SABM with insulin ...
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bioRxiv - Cell Biology 2023Quote: Contact activation and tissue factor-induced thrombin generation times (TGT) were performed in Nunclon Delta Surface 96 well microtiter plates (Thermo Scientific).31 The assay was performed in 25 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... (74) were cultured in keratinocyte serum-free media (KSFM) supplemented with bovine pituitary extract and human epidermal growth factor (Thermo Fisher). HCT116 human colon carcinoma lines were obtained from ATCC and maintained following their recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... spheres were plated as neural progenitors onto a 10cm dish precoated with growth factor reduced (GFR) Matrigel (CB-40230A, Fisher Scientific). Neural progenitors were allowed to form neuronal rosettes and sustained in N2B27 media alone for days 8–15 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 40 min at 4°C using the eBioscience™ Foxp3/Transcription Factor Fixation kit (Thermo Fisher Scientific) and permeabilized using the kit’s permeabilization buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... with 50,000 mouse endothelial cells (harvested from mouse lungs by CD31 selection and expanded in vitro as previously described 67) and resuspended in 50 µl of Geltrex™ reduced growth factor basement membrane matrix (A1413301, Gibco). 100 µl of 1:1 GFP+:endothelial cell mixture was plated on transwell inserts with 0.4 µm pores and allowed to solidify for 30 mins in a humidified CO2 incubator (EOE ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Intranuclear and intracellular marker were stained after fixation and permeabilization of cells with the FoxP3/Transcription Factor staining buffer set (Thermo Fisher) or Cytofix/Cytoperm (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Cells that received an intracellular stain were fixed and permeabilized using the FOXP3/Transcription Factor staining buffer set (Thermo Fisher Scientific). Flow cytometry data were collected on the LSRFortessa X-20 ...
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bioRxiv - Immunology 2024Quote: ... Parallel co-cultures were kept for one week to assess expression of FOXP3 and HELIOS (Stability) by intracellular staining using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience, ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were first surface stained as described above and fixed (eBioscience Transcription Factor Staining Set; Thermo Fisher Scientific, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Individual strips with a small amount of medium were then carefully moved to pre-chilled micro-centrifuge tubes and then mixed with cold reduced growth factor basement membrane matrix Geltrex (Thermo Fisher). The dilution of Geltrex by the introduced medium was less than 20% ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized and stained with indicated antibodies using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific). Data were acquired on a BD LSRII or Fortessa cytometer ...
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bioRxiv - Cancer Biology 2024Quote: ... 150,000 KPARG12C or 400,000 3LL-ΛNRAS cells were mixed 1:1 with Geltrex LDEV-Free Reduced Growth Factor matrix (Thermo Fisher Scientific) and injected subcutaneously into one flank ...
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bioRxiv - Immunology 2023Quote: ... All cells were then fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (00-5523-00, ThermoFisher), as per manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed and permeabilized using FOXP3 Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Catalog no. 00-5523-00) and stained for intracellular markers RORψt anti-mouse PE-CF594 (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Rosettes were plated on growth factor reduced matrigel coated 6 well plates in ‘pNPC medium’ composed of a 1:1 mixture of Neurobasal (ThermoFisher Scientific) and DMEM/F12 ...
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bioRxiv - Immunology 2022Quote: BAL and NT samples were stained in 96-wells plates using the FoxP3 / Transcription factor staining buffer set (eBioscience, Thermo Fisher). Cells were stained with α-CD4-APC (02 ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were cultured in basement membrane extract (BME, Trevingen) and overlaid with growth medium consisting of murine recombinant epidermal growth factor (EGF 50 ng/ml, Life Technologies), murine recombinant Noggin (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Cancer Biology 2022Quote: Organoid growth was evaluated in growth factor–free (GF-free) medium (Advanced DMEM/F12 [Gibco, Thermo Fisher Scientific, Waltham, MA, USA] supplemented with 1x GlutaMax [Gibco]) or StemPro medium (StemPro hESC [Invitrogen] ...
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bioRxiv - Bioengineering 2022Quote: ... and stained with primary antibodies (1:200 CD31; Dako IS610 or 2.5 μg/mL von Willebrand Factor viii; Invitrogen MA5-14029) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed in FACS buffer and fixed and permeabilized with the Foxp3 / Transcription Factor Staining Buffer Set (00-5523-00, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed once with FACS buffer and subsequently fixed for 30 minutes with fixation/permeabilisation buffer of eBioscience Foxp3/transcription factor staining buffer set (ThermoFisher Scientific). They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4 ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were grown also on plates coated Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Immunology 2023Quote: ... the cells were fixed/permeabilized overnight at 4°C in dark using eBioscience FOXP3 transcription factor staining buffer set (cat. #00-5523-00; Thermo Fisher) and FOXP3 monoclonal antibody (1:25 dilution ...
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bioRxiv - Immunology 2023Quote: ... surface-labelled cell suspensions were fixed using eBioscience Foxp3/Transcription Factor Staining Buffer Set or eBioscience IC Fixation Buffer (both from Thermo Fisher). Cell suspensions were stained with following antibodies at 4°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 0.01 ng/ml human epidermal growth factor (Pep Rotech, AF-100-15) and 1x Penicillin-Streptomycin (Gibco™, Cat. No. 15140122).
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bioRxiv - Cancer Biology 2024Quote: ... A 50 μL solution of 1:1 growth-factor-reduced Matrigel:serum-free DMEM ± 1:40 DQ-Collagen IV (Thermo Fisher Scientific) was added above the monolayer and allowed to form a gel ...
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bioRxiv - Immunology 2024Quote: ... Fixation and permealization was done using the eBioscience Foxp3/Transcription Factor Staining buffer set (ThermoFisher Scientific, catalogue no. 00-5523-00). Cells were acquired on BD FACSymphony A5 SE and Cytek Aurora and analyzed in FlowJo (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... Cells were fixed and permeabilized with the eBioscience™ FOXP3/Transcription Factor Staining Buffer Set (Invitrogen; Cat. No. 00-5523-00), followed by intracellular staining with fluorochrome-conjugated antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... chemokines and growth factors in 14 supernatant samples by Luminex using the Cytokine Human Magnetic 30-Plex Panel LHC6003M from Life Technologies™ ...
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bioRxiv - Immunology 2024Quote: ... CD45R/B220 BV510) and then fixed and permeabilized using the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen #00-5523-00) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Human dermal fibroblast-derived hiPSCs were cultured in feeder-free conditions on Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (A1413201, Gibco) diluted 1:100 in ice cold Advanced DMEM/F-12 (12634010 ...
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bioRxiv - Neuroscience 2024Quote: ... TMT16 reporter ion intensities were corrected for isotopic impurities in the Spectrum Mill protein/peptide summary module using the afRICA correction method which implements determinant calculations according to Cramer’s Rule and correction factors obtained from the reagent manufacturer’s certificate of analysis (Thermo Fisher Scientific, 90406) for lot numbers VH310017 for both synapse proteomics experiments ...
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bioRxiv - Genetics 2024Quote: ... for 30 min at 4 °C prior to fixation and permeabilization (FOXP3/Transcription Factor Staining Buffer Set, 00– 5523, ThermoFisher Scientific) for 30 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... For intracellular staining cells were fixed and permeabilized using the eBioscience Foxp3 Transcription Factor Staining Buffer Set (Cat# 00-5523-00, ThermoFisher Scientific) followed by intracellular staining with the desired antibody overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... were cultured at 37⁰C with 5% CO2 on plates coated with 75µg/ml Geltrex™ LDVE-Free Reduced Growth Factor Basement Membrane Matrix (Gibco A1413292). Cells were maintained in KnockOut™ DMEM/F-12 (12660012) ...
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bioRxiv - Cell Biology 2024Quote: ... They were cultured in Keratinocyte serum-free medium (SFM) supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (17005042, Life Technologies) at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Intracellular marker staining was performed using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, #00-5523-00). Data acquisition was conducted using an Attune NxT Flow Cytometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Annexin V-Alexa Fluor488 (used at 1 μg/mL) and eBioscience™ FOXP3/Transcription factor staining buffer set (Cat.00-5523-00) were from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.5×106 wild-type or pre-transduced VA-ES-BJ or NEPS EpS cells suspended in a 1:1 mix of PBS and Geltrex (LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix-5 mL. A1413302, Gibco/LifeTechnologies) were subcutaneously injected into the flank of NSG mice ...