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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 1 μM Fluo-4-AM (Thermo Fisher) in culture medium and incubated at 37 °C and 5% CO2 for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... were used at 4°C overnight: anti-GFP (1:250, Invitrogen), anti-DsRed(RFP ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal anti-Claudin-4 (1:100, Novex, Life Technologies, USA) and rabbit polyclonal anti-claudin-4 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:4 dilution of trypsin was used (Gibco, Cat# 15400054) to split the cells into thirds.
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bioRxiv - Bioengineering 2022Quote: ... eBCs were stained with polyclonal Synaptotagmin-4 antibody (1:50; Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Bioengineering 2020Quote: dLNs were fixed for 1 hour with 4% paraformaldehyde (Thermofisher Scientific), embedded in Tissue-tek OCT (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... GPC-4 (#13048-1-AP, Thermo Scientific, and #MABN528, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Aliquots of 4 µL of 1 mM Mitotracker Green FM (ThermoFisher) stock solution were added to the samples and were kept in the dark at 15°C for 15–20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-FABP-4 (Thermo Fisher Scientific, PA5-30591), 1:200 rabbit anti-Osteocalcin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the aliquots were incubated with 4-pentyn-1-o1 (ThermoFisher Scientific) and ADPRC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... KP-4 and PANC-1 cells were maintained in DMEM (Gibco). LOX-IMVI cells were maintained in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... ki67 (ThermoFisher, Cat. RM-9106; 1:50 overnight at 4 °C), βIII-tubulin (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-HuC/D (Elavl3/4) (1:500, Invitrogen A21271) in PBST overnight at 4 °C in a humidified chamber ...
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bioRxiv - Microbiology 2024Quote: ... A 1:4 dilution of Intracellular Fixation Buffer (Invitrogen, Waltham, MA) was added to each chamber ...
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bioRxiv - Microbiology 2023Quote: ... plus DAPI (Thermo Fisher Scientific, 1:1000 in 4% BSA PBS) at room temperature ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: Astrocytes were loaded with Fluo-4 AM (1 mM; Molecular Probes) in culture media for 20 min at 37°C followed by 10 min wash in HBSS (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of 1 μM Fluo-4 AM (ThermoFisher Scientific, F14021) in GBO medium was added to each GBO at the ALI surface for at least 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... one well of a 6 well plate was transfected with 2 μg of plasmid DNA with Lipofectamine® 2000 (Life Technologies™) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Chlorophyll content (chlorophyll a and b) was quantified with the NanoDrop One Spectrophotometer (ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μg/ml Amphotericin B (Amp B, Thermo Scientific) was added to the medium from day 10 onwards ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were seeded into 12-well plates and were transfected with 1.5 μg the RPL plasmid and 0.5 μg Cas13b gRNAs (NTC, U1-1, U1-2, U1-3) while ∼80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, Cat.# L3000015). For RIP experiments ...
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bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Immunology 2022Quote: ... Each antibody (2-4 µg per reaction) and IgG control were incubated with Dynabeads (Invitrogen) for at least 4 h at 4C with rotation ...
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bioRxiv - Neuroscience 2019Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Biochemistry 2020Quote: ... the DNA was digested by addition of 2 µL/4 U Turbo DNase (Ambion, AM2238) for 15 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... fixed using 4% paraformaldehyde and blocked with PBS containing 2% bovine serum albumin (BSA) (Invitrogen) and 0.1% normal goat serum (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... MCF7 cells were treated with 400 µM 4-thiouridine for 2 hours (4sU, Fisher Scientific). After labeling ...
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bioRxiv - Microbiology 2023Quote: ... The crosslinked membrane was prehybridized at 62°C for 2-4 hours in ULTRAhyb (Ambion) buffer and then labeled probe was added for overnight incubation ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C containing 2% v/v antibiotic-antimycotic (anti-anti, 15240, Thermo Fisher Scientific). The bone part was cut perpendicular to the longitudinal axis using a bench saw (KS230 ...
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bioRxiv - Neuroscience 2023Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Biochemistry 2023Quote: ... at 4°C before centrifugation and bead removal using a DynaMag™-2 Magnet (Invitrogen). Supernatants mixed 1:1 with Co-IP elution buffer (2X NuPAGE® LDS ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 4°C for 45 min with 2 μg/mL Alexa647 (Life Technologies)-conjugated M1 anti-FLAG (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated 2-4 hrs in Earle’s balanced salt solution (EBSS; Gibco, # 24010043). Bafilomycin A1 (referred as BafA1 ...
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bioRxiv - Cell Biology 2024Quote: ... Either 4 nM WR99210 (Jacobus Pharmaceuticals) or 2 μg/mL blasticidin S (Life Technologies, USA) was used for selecting transfectants ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µl of 5M NaCl and 2 µl of RNaseA (10 mg/mL) (ThermoFisher, # EN0531) were added to each sample and the samples were then incubated at 65°C with shaking overnight.
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...