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Citations for Thermo Fisher :
251 - 300 of 368 citations for Thiamine EP Impurity A Chloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... dissolved in chloroform and supplemented with 0.2 mol % octadecyl rhodamin B chloride (R18, Thermo Fisher) were vacuum dried on a rotary evaporator ...
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bioRxiv - Biochemistry 2024Quote: ... followed by red blood cell lysis with ammonium-chloride-potassium lysing buffer (Thermo Fisher Scientific)43 ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified with lithium chloride precipitation followed by the Megaclear Kit (Ambion cat #AM1908). 1 nL injection mix containing the bact2:HaloTag-NES-2A-mCerulean-CAAX plasmid DNA at 25 ng/µl and Tol2 mRNA at 25 ng/µl was injected into zebrafish AB wild-type embryos in the cell at the one-cell stage ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Massachusetts, US) for 5 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... samples were resuspended in an ammonium-chloride-potassium red blood cell lysis buffer (Thermo Fisher Scientific) and centrifuged again ...
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bioRxiv - Genetics 2020Quote: ... followed by lysis of red blood cells using Ammonium-Chloride-Potassium (ACK) lysis buffer (Gibco, A1049201), washing with Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Microbiology 2021Quote: ... Red blood cells were lysed via incubation with ammonium-chloride-potassium (ACK) lysis buffer (A1049201; Gibco) for 5 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Pooled subgingival microbial samples were resuspended in 0.85% sodium chloride buffer (Thermo-Fisher Scientific, Waltham, MA) and grouped in live (stained only with Syto9 ...
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bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed using an Ammonium-Chloride-Potassium (ACK) lysis buffer (Thermo Fisher Scientific). Fifty thousand Trp1 CD4+ T cells in PBS were adoptively transferred via the tail vein into B16F10 melanoma-bearing mice ...
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bioRxiv - Cell Biology 2023Quote: ... Magnesium 1 M (cat: AM9530G) and Sodium chloride 5 M (cat: AM9759) were obtained from Ambion. Ultrapure water (cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were suspended in 75 µL High Salt buffer (3 M sodium chloride, NaCl, Fisher Scientific; 25 mM dithiothreitol ...
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bioRxiv - Bioengineering 2024Quote: ... The weight of the initial gel was measured before a sodium chloride (NaCl, Fisher Scientific, S271) solution was added for 12 h ...
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bioRxiv - Bioengineering 2020Quote: Pentaerythritol, methacryloyl chloride, trimethylamine, tetrahydrofuran (THF, extra dry) and acetone (extra dry) were purchased from Acros Organics. ε-caprolactone (CL ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... RNA was then purified via lithium chloride precipitation by addition of LiCl Precipitation Solution (Invitrogen, Carlsbad, CA) to reach a final concentration of 2.5 M LiCl ...
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bioRxiv - Microbiology 2020Quote: ... H2O2 and copper chloride were removed using a Zeba Spin 7K MWCO desalting centrifuge column (Fisher Scientific) pre-equilibrated with the original Rucred storage buffer ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: 100 mg AG was dissolved in pyridine and 450 mg p-TsCl (p-Tosyl Chloride, Acros Organics) reagent was added ...
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bioRxiv - Cancer Biology 2023Quote: ... Red cell lysis was performed in 10 ml ACK (Ammonium-Chloride-Potassium) lysing buffer (Invitrogen, Cat # 11814389001) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... containing acid citrate dextrose (ACD) was combined with 2.75% Calcium Chloride (Fisher Scientific, Fair Lawn, NJ, USA) in a 10:1 ratio (100 mL blood/10 mL CaCl2 ...
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bioRxiv - Immunology 2023Quote: ... Erythrocytes were lysed for 3 min using an ammonium chloride-potassium phosphate buffer (ACK Lysing Buffer, Gibco). Cell numbers were counted in Turk’s solution using a Neubauer chamber.
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bioRxiv - Cancer Biology 2022Quote: ... Bone marrow cells and splenocytes were resuspended in 1mL Ammonium-Chloride-Potassium (ACK) Lysis buffer (Gibco, Invitrogen) and placed on ice for 5 min to lyse red blood cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Bone marrow cells and splenocytes were resuspended in 1mL Ammonium-Chloride-Potassium (ACK) Lysis buffer (Gibco, Invitrogen) and placed on ice for 5 min to lyse red blood cells ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in 1mL ammonium-chloride-potassium (ACK) red-cell lysis buffer (A1049201, Life Technologies) and kept on ice for 3 min ...
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bioRxiv - Physiology 2022Quote: ... Single cells were resuspended in 1 ml of ACK (ammonium-chloride-potassium) lysis buffer (Thermofisher Cat# A1049201) for 2 minutes at room temperature and washed with 2 mL of FACS buffer— 1% fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: Mice were sacrificed and immediately perfused by hand pressure with 0.89% normal saline (sodium chloride, Acros Organics) using an infusion wing set with 23GX3/4 gauge needle with 12” tubing (Terumo) ...
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bioRxiv - Immunology 2024Quote: ... and the remaining red blood cells were removed using ammonium-chloride-potassium lysis buffer (Gibco, #A10492-10). After two additional washing steps using PBS ...
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bioRxiv - Microbiology 2024Quote: ... Concentrations of chloride (Cl-) and bromide (Br-) were measured by an ICS-1100 ion chromatography (Thermofisher, USA) with an analytical error of ± 1% ...
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bioRxiv - Biophysics 2022Quote: ... The conductivities of each alkali chloride were recorded using an Accumet AB200 pH/Conductivity Benchtop Meter (Fisher Scientific). The results were as follows ...
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bioRxiv - Bioengineering 2022Quote: ... filtered trypan blue solution (0.4% trypan blue in 0.81% sodium chloride and 0.06% dibasic potassium phosphate; Life Technologies) was prepared and incubated for 2 min at room temperature for viable/non-viable cell imaging under the phase contrast microscope ...
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bioRxiv - Bioengineering 2020Quote: ... EVs are either non-specifically labeled using the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (Life Technologies, catalog# O246 ...
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bioRxiv - Microbiology 2020Quote: ... diluted in Earle’s balanced salt solution supplemented with 200 mg/ml Calcium Chloride (Gibco, Life Technologies, Renfrew, UK), at 37°C with continual gentle mixing of the tube ...
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bioRxiv - Microbiology 2020Quote: ... diluted in Earle’s balanced salt solution supplemented with 200 mg/ml Calcium Chloride (Gibco, Life Technologies, Renfrew, UK), at 37°C with continual gentle mixing of the tube ...
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bioRxiv - Microbiology 2020Quote: ... in ice-cold RIPA buffer (150 mM sodium chloride, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0; Thermo Fisher Scientific), supplemented with complete Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once with Krebs-Ringer’s buffer containing 1.8 mM calcium chloride and loaded with Fluo-4 AM (1 μg/μl; ThermoFisher) plus 10 μl 0.2 % pluronic acid for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
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bioRxiv - Physiology 2023Quote: ... The pellets were then resuspended and incubated with ammonium-chloride-potassium (ACK) lysing buffer (Thermo Fisher Scientific, USA) for 3 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... The virus was then dialyzed with 1× phosphate buffered saline (pH 7.2) supplemented with 50 mM sodium chloride and 0.0001% Pluronic F68 (Thermo Fisher) using 50 kDA filters (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... was accomplished by reacting the respective oligosaccharide glycamines with Lissamine rhodamine B sulfonyl chloride (LRSC) (Acros Organics, Belgium, USA) as described previously (64) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by Poly(A) tailing procedure and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). pCMV-ancBE4max (pCMV_AncBE4max was a gift from David Liu (Addgene plasmid # 112094 ...
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bioRxiv - Microbiology 2020Quote: Approximately 5 μg of BAC DNA was linearized with NotI and full-length RNA was obtained by in vitro transcription with T7 RNA polymerase followed by lithium chloride precipitation according to the manufacturer’s protocol (mMessage-mMachine T7 Kit; Ambion). 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed and stained with 200uL of 1 mg/mL solution of Nitro Blue Tetrazolium Chloride (Invitrogen, N6495). Six biological replicates were performed ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Microbiology 2023Quote: ... Red blood cells (RBCs) were then lysed by resuspending cells in ammonium-chloride-potassium (ACK) lysis buffer (Thermo Fisher) for 2 min ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and pellets were resuspended in Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Catalog # A1049201) to lyse RBC (erythrocytes removal performed at R.T ...
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bioRxiv - Molecular Biology 2024Quote: ... We discarded the supernatant and resuspended the cell pellet in 1mL of Ammonium-Chloride-Potassium (ACK) lysis buffer (Thermofisher) and incubated for 30 seconds at room temperature to lyse erythrocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with octadecyl rhodamine B chloride (R18, 1:1,000 dilution, stock 10mg mL-1; Thermo Fisher Scientific) prior to 3D cell encapsulation.
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated in a 48 °C prewarmed saline-sodium citrate wash buffer (30 mM sodium citrate, 300 mM sodium chloride, pH7.4, Invitrogen, France) for 20 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthesized by in vitro transcription with 1μl of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). the pCS2+_ancBE4max-SpymacCas9 has been linearized using KpnI restriction enzyme ...
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bioRxiv - Immunology 2021Quote: ... Spleens and lungs were mashed through a 70-μm strainer before red blood cells were lysed using ammonium-chloride-potassium (ACK) lysis (Gibco). Cells were washed with PBS containing 1% FCS and surface receptors were stained using various antibodies ...