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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Mouse S100A8 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Cancer Biology 2020Quote: ... the fusion proteins were diluted in mouse serum (Invitrogen) to 200 nM and incubated at 37°C for up to 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... or control mouse IgG and protein G Dynabeads (Invitrogen). Immunocomplexes were eluted by denaturation in NuPAGE LDS sample buffer (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Evolutionary Biology 2023Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... or Protein G (Thermo Fisher Scientific, Cat. #10002D, for mouse). HEK293T lysates were supplemented with 1.5 mM CaCl2 and 1 μM calmodulin (final concentrations ...
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bioRxiv - Systems Biology 2021Quote: ... immunoprecipitated proteins were detected with monoclonal mouse anti-V5 (Invitrogen) or mouse anti-HA.11 (BioLegend ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding mouse PRMT proteins were subcloned into pFASTBAC1 (Invitrogen) with a FLAG tag ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Cells were lysed by sonication and recombinant proteins purified by Ni2+ affinity chromatography in Ni-NTA agarose (Thermo Fisher) using 50 mM TrisHCl/500 mM NaCl/10 mM imidazole [ph 8.0] as binding buffer ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purity of recombinant proteins was verified by SDS-PAGE with staining by GelCode Blue stain reagent (Thermo Fisher scientific).
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bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...