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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Mouse T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells were combined 1:1 and stimulated with CTS™ Dynabeads™ CD3/CD28 (Invitrogen) at a 1:1 ratio overnight in X Vivo-15 (Lonza ...
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bioRxiv - Immunology 2023Quote: ... T cells were first stained with surface markers togerher with LIVE/DEAD Fixable Near-IR (Cat# L34976, Invitrogen) at 4 °C for 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... T cells were cultured with mouse CD3/CD28 Dynabeads (Invitrogen) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were either cultured alone or with T cell activation beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Thermo Fisher Scientific, 11456D). After 72 hrs of culture ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were either cultured alone or with T cell activation beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Thermo Fisher Scientific, 11456D). After 72 hrs of culture ...
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bioRxiv - Immunology 2021Quote: Primed CD8+ T cells isolated from WT or Dok1/Dok2 DKO mice were loaded with Cell Trace Violet Stain (Life Technologies #C34557) or Cell Trace Far Red DDAO (Life Technologies #C34553) ...
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bioRxiv - Cancer Biology 2020Quote: ROS production from CD8+ T-cells following MDSC-derived exosomes treatment in vitro was estimated by labeling the CD8+ T-cells using CM-H2DCFDA (Invitrogen™, C6827). In short CD8+ T-cells were isolated according to the above-mentioned method ...
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bioRxiv - Immunology 2021Quote: ... and CCL2 were measured using Mouse Uncoated ELISA Kit (Invitrogen, Vienna, Austria) and mouse CXCL1/KC DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... and IgA concentration was measured by mouse IgA uncoated ELISA kit (Invitrogen) or mouse IgA ELISA Quantification Set (#E90-103 ...
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bioRxiv - Cancer Biology 2023Quote: Mouse TNF-α uncoated ELISA Kit from Invitrogen (Ref 88-7324-22) was used according to manufacturer’s instructions to quantify TNF-α concentration in 2ml supernatants from confluent 6-wells of keratinocyte cultures after 24 h of incubation ...
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bioRxiv - Immunology 2023Quote: ... IL-18 was assayed using the IL-18 Mouse ELISA Kit (Invitrogen) according to the instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Activated CD3+ T cells were isolated from Dynabead Mouse T Activator CD3/CD28 (#00775477, Thermo Fisher) using negative selection ...
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bioRxiv - Microbiology 2021Quote: ... 5-8×103 CD8+ T cells from lamina propria or 15-20×103 CD8+ T cells from tumors were processed for the RNA extraction using the Arcturus® PicoPure® RNA Isolation Kit (Thermo Fisher, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells used for transduction were obtained by magnetic separation from healthy-donor derived apheresis products (Dynabeads® Untouched™ Human CD8+ T Cells Kit, Thermo Fisher; Magnet, DynaMag™).
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bioRxiv - Cell Biology 2021Quote: ... using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific, 89881). Sulfo- NHS-SS-Biotin was dissolved in PBS supplemented with MgCl2 (0.5 mM ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated using a commercial kit (MagniSort Human CD4+ T Cell Enrichment; Affymetrix) and infected by incubation for 4 h at 37°C with 156,250 or 2,500 TCID50 (50% tissue culture infectious dose ...
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bioRxiv - Immunology 2019Quote: ... total CD4+ T cells were isolated using the Dynabeads CD4 positive T cell isolation kit (Invitrogen). Purified cells were 95%–98% pure as determined by flow cytometry analysis ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Zoology 2019Quote: ... and phycoerythrin (PE)-labeled anti-mouse CD8 (Invitrogen, USA). FITC- ...
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bioRxiv - Genetics 2020Quote: ... cells were surface stained with cell-viability dye (Fixable Blue dead cell stain kit, Invitrogen), followed by anti-CD3-BV711 (BioLegend) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then stained with triple-washed anti-IgG magnetic beads (Untouched Mouse T Cells Kit, Thermo Fisher) rotating at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: MesLNs were collected and CD4+ T cells isolated using the Dynabeads™ Untouched™ Mouse CD4 Cells Kit (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... incorporation of glucose and fatty acid metabolism was determined by flow cytometry analysis of isolated CD8+ T cells from PLWH and control donors stained with 2-NBDG (Invitrogen) and 500/510 BODIPY (Molecular Probes ...
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bioRxiv - Immunology 2019Quote: Intracellular ATP levels were measured ex vivo on sorted CD4+ and CD8+ T cell subsets via a bioluminescence assay according to the manufactures instructions (ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... naïve CD8+ T-cells were isolated from an enriched PBMC fraction and activated with αCD3 and αCD28 beads (Dynabeads, Invitrogen) for 48 hours ...
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bioRxiv - Bioengineering 2019Quote: CD8+ and CD4+ T cells were purified from PBMC and activated by stimulation with anti-CD3/anti-CD28 beads (Thermofisher) in separate cultures ...
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bioRxiv - Genetics 2019Quote: ... Non-adherent cells in suspension from resting and PHA-stimulated cultures were removed and purified for CD8−/CD4+ T cells using Dynabeads (Invitrogen) and stored in RNAprotect Cell reagent (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... non-naïve (CCR7+CD45RA-, CCR7-CD45RA- and CCR7-CD45RA+) CD3+CD8+ T cells were directly sorted into TRIzol reagent (Thermo Fisher) on a FACSAriaFusion (BD ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... Nuclear and cytoplasmic extracts from CD8+ T cells were obtained with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, ThermoFisher). The Revert 700 Total Protein Stain (LICOR ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Cell Biology 2024Quote: Surface immunophenotyping was carried out on cells blocked in 10% normal mouse serum (Invitrogen) in PB2 and then stained with the antibodies (Supplemental Table 1) ...
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bioRxiv - Immunology 2024Quote: ... Cells were activated using Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (ThermoFisher, Cat: 11456D) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Wild-type C57BL/6 splenic-T cells are isolated using Stemcell EasySep™ Mouse T-cell Isolation kit (Stemcell #19851) and stained with Cell Trace Violet (ThermoFisher #C34571). These cells are then stimulated with anti-CD3/CD28 beads (Gibco #11456D ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation of CD4+ and CD8+ T cells was induced by stimulating cells with plate bound anti-CD3 [0.1 µg/mL] (Thermo Fisher Scientific, MA, USA) and soluble anti-CD28 [1 µg/mL] (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Mouse IFNg ELISA (Thermo Scientific, Cat.KMC4021) were performed according to the instruction ...
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bioRxiv - Immunology 2022Quote: ... T cells were purified from splenocytes using a negative selection kit (MagniSort T cell enrichment, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-beta actin (BA3R) monoclonal antibody (ThermoFisher Scientific). Species- and/or mouse isotype–specific secondary antibodies from donkey or goat and conjugated to Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA kits according to the manufacturer instructions (Mouse IFN gamma Uncoated ELISA, Invitrogen, #88-7314 ...
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bioRxiv - Microbiology 2021Quote: ... After 4x washing with PBS-T the ELISA was developed with 1-step Ultra TMB ELISA (Thermo Scientific). For generation of an oxMIF standard curve ...
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bioRxiv - Immunology 2022Quote: Mouse serum IgG and IgG2a were detected using commercially available ELISA kits (ThermoFisher) as per manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... or IL-2 Mouse Uncoated ELISA Kit (Thermo Fisher Scientific, 88-7024-76) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were isotyped using the Rapid ELISA Mouse mAbs Isotyping Kit (ThermoFisher Pierce) with anti-mouse heavy chain capture antibody (anti-IgG1 ...
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bioRxiv - Immunology 2021Quote: Cytokine concentration was measured by using IL-6 Mouse Uncoated ELISA Kit (Invitrogen) according to manufacturer’s instructions ...