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Citations for Thermo Fisher :
251 - 300 of 8852 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Immunology 2021Quote: ... MIP-1b(CCL4)), growth factors (BDNF, G-CSF, GM-CSF, PDGF-BB, VEGF-A) and other factors (CD40L, Granzyme B) (Invitrogen, Carlsbad, CA). Differences in induction of proteins post stimulation were tested using unpaired t-tests with Welch’s correction ...
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bioRxiv - Immunology 2021Quote: ... MIP-1b(CCL4)), growth factors (BDNF, G-CSF, GM-CSF, PDGF-BB, VEGF-A) and other factors (CD40L, Granzyme B) (Invitrogen, Carlsbad, CA). Differences in induction of proteins post stimulation were tested using both unpaired (Control-EtOH ...
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bioRxiv - Immunology 2021Quote: ... and GM-CSF using custom ProcartaPlex assay kits (Invitrogen). Analyte concentrations were determined using a Luminex100/200 Flexmap3D instrument coupled with Luminex XPONENT software (Luminex ...
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bioRxiv - Immunology 2020Quote: ... This was followed by induction with GM-CSF (Invitrogen) 5 ng/ml for 4 days which helps in the differentiation of BM-MNC to dendritic cells ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were cultured with GM-CSF (Thermo Fisher, PHC2013) and M-CSF (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 M HEPES (1 M HEPES, Gibco) in PBS for 20 min at 37°C ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HEK293 cells were cultured in DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 cells were cultured in DMEM medium (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: HEK293 cells were maintained in DMEM plus Glutamax (Gibco) supplemented with 10% heat-inactivated FBS in a humidified 37°C ...
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bioRxiv - Immunology 2021Quote: ... Lentiviral particles were produced in HEK293-FT cells (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were expressed in HEK293 Freestyle cells (Invitrogen) in suspension culture using serum-free media (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... We cultured HEK293 cells in DMEM medium (Gibco, #11965092) with 10% fetal bovine serum and 1% penicillin– streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... transfected with 293fectin into FreeStyle HEK293-F cells (Invitrogen), and purified from culture supernatants by sequential HisTrap Ni2+-NTA and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells (ATCC CCL-2) and HEK293-FT (ThermoFisher) were cultured at 37 °C in 5% CO2 in medium composed of high glucose Dulbecco’s modified eagles medium (DMEM ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 cells were cultured in DMEM (Gibco; Life Technologies) with 10% FBS (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 cells were cultured in DMEM (Gibco; Life Technologies) with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 Flp-In™ T-Rex™ cells (Invitrogen) or HEK293T cells (ATCC ...
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bioRxiv - Genomics 2021Quote: HEK293 cells (ATCC) were cultured in DMEM (ThermoFisher Scientific) with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2021Quote: ... Polyclonal HEK293 Flp-In T-REx cell lines (Invitrogen) were generated as described previously (Wyler et al. ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were dissociated with TrypLE (Thermo Fisher Scientific), washed ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells were transfected using Lipofectamine 3000 (Thermo Fisher). SERCA2a constructs were expressed using the mammalian expression vector pcDNA3.1 with G418 resistance ...
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bioRxiv - Cell Biology 2020Quote: ... Flp-In T-Rex HEK293 (R78007, Thermo Fisher Scientific) and HaCaT (obtained from Joan Massague’s lab at Memorial Sloan Kettering Cancer Centre ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTI- cells cultured in Freestyle 293 medium (GIBCO) supplemented with 2% FBS at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... for HEK293 FT cells and Lipofectamine 2000 (Thermo Fisher) for fibroblast cell lines ...
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bioRxiv - Molecular Biology 2020Quote: HeLa S3 and HEK293 T-REx (Thermo Fisher Scientific) cells were maintained in DMEM supplemented with 10% FBS in a humidified incubator at 5% CO2 and 37°C ...
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bioRxiv - Genomics 2021Quote: HEK293 LTV cells were cultured in DMEM medium (Gibco), supplemented with 10% FBS at 37°C ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: The HEK293 cells were maintained in DMEM medium (Gibco) supplemented with 10% FBS and 50 U penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transiently transfected using Lipofectamine 3000 (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... We cultured HEK293 cells in DMEM medium (Gibco, #11965092) with 10% fetal bovine serum and 1% penicillin–streptomycin ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and Jurkat cells were maintained in RPMI (Gibco) media containing 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were cultured in Advanced DMEM (Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Genomics 2023Quote: HEK293 cells were transfected with Lipofectamine 2000 (Invitrogen, 11668019) at a ratio of 2.8 ul lipofectamine per ug of DNA ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were maintained in DMEM medium (Gibco, USA) supplemented with 1% penicillin-streptomycin solution (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Flp-In T-REx HEK293 cells (Thermo Fisher Scientific) were co-transfected with this expression vector and pOG44 Flp-recombinase expression vector in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and A549 cells were maintained in DMEM (Invitrogen) supplemented with 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were resuspended in buffer R (Thermo Fisher), along with the RNP complex and ssODN template ...