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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for 5 5 7 7 Tetramethyl 1 5 6 7 tetrahydrocyclopenta f indazol 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: 10 uL of either Caspase-3 or −7 library glycerol stocks was used to inoculate 5 mL of auto-induction media (Invitrogen Magic Media) and allowed to incubate and express for 18 hours at 30 °C ...
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bioRxiv - Neuroscience 2020Quote: We dissected brains and ventral nerve cords of 5-7 days old males in Schneider’s insect medium (Thermo Fisher Scientific, Waltham, MA) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2021Quote: ... For the analysis of STAT3/5 phosphorylation cells were also labeled with phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated anti-phospho-STAT3 (Tyr705) (Thermo Fisher Scientific) (#25-9033-42 ...
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bioRxiv - Biochemistry 2020Quote: ... QPCR was performed on standards of diluted genomic DNA and cDNA products using specific primers (Supplemental table 5) on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Galnts gene copy numbers were calculated using the genomic DNA as a standard curve and variation between biological replicate was normalized using Actb expression level.
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were run on the QuantStudio 6 or 7 (ThermoFisher Scientific, Waltham, MA) using the qPCR reaction settings as follows ...
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bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
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bioRxiv - Genomics 2024Quote: MCF-7 cells were cultured in in 75 cm2 flasks DMEM/F-12 (ThermoFisher 10565018) supplemented with GlutaMAX (Gibco 10565018) ...
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bioRxiv - Microbiology 2021Quote: ... 0.5-1×10^7 parasitized RBCs were incubated with 4 µM BCECF-AM (B1170, ThermoFisher) and 0.02% Pluronic F-127 (p6867 ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA) and Direct-Zol RNA Mini Prep (Zymo Research ...
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bioRxiv - Biophysics 2022Quote: African green monkey kidney cells (COS-7) were cultured at 37°C and 5 % CO2 in DMEM medium containing glutamax (Gibco No. 31966-047), 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Hepa 1-6 and HuH-7 were grown in Dulbecco’s modified Eagle’s medium–high glucose (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then resuspended in aCSF and incubated with DAPI (1:1000 dilution of 1mg/mL DAPI, Thermo Fisher, #62248 or 7-AAD 7-Aminoactinomycin D, A1310, Thermo Fisher) for FACS sorting ...
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bioRxiv - Immunology 2022Quote: ... Design and Analysis QuantStudio 6/7 Pro Systems Software (Thermo Fisher Scientific, Version 2.5.0) was used to identify amplification of genes and calculate fold change from Cq values ...
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bioRxiv - Immunology 2020Quote: ... The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+) were digested with Proteinase K (Invitrogen) at 55°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 110 nm ultrathin cryosections were collected on 7×7 mm silicon wafers with fluorescent beads (PS-Speck, ThermoFisher). Light microscopy images were acquired with a widefield microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... Caspase-3/7 was quantified using the Apo-ONE® Homogeneous Caspase-3/7 Assay (Promega, Fisher scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorogenic probe 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (abbr. CPM, ThermoFisher, Cat# D346) in 100% DMSO was then added to both quench the enzymatic reaction and react with CoASH to yield the fluorescent CPM-SCoA complex for fluorescence quantification 27 ...
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bioRxiv - Neuroscience 2022Quote: ... mixed with 5% biotinylated dextrane amine (BDA, MW: 10.000, Molecular Probes: D1956 ...
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bioRxiv - Neuroscience 2021Quote: ... TMT10-plex amine reactive reagents (Thermo Fisher, 5 mg per vial) were re-suspended in 1024 μL anhydrous acetonitrile and 25 μL of reagent was added to each sample (TMT label ...
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bioRxiv - Neuroscience 2020Quote: ... or 4′,6-diamidino-2-phenylindole (DAPI, 5 μg/ml, Invitrogen) were included in the secondary antibody solution to stain nuclei.
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bioRxiv - Neuroscience 2023Quote: ... or 4°,6-diamidino-2-phenylindole (DAPI, 5 μg/ml, Invitrogen) were added during the first wash step to visualize nuclei ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then 1 h at 4°C at 7-10 rpm with dynabeads protein A (Invitrogen), equilibrated with the EB ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Cell Biology 2020Quote: ... using the ViiAtm 7 platform (Applied Biosystems). Relative quantification was conducted by either SYBRgreen fluorescent dye (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... 7 mM MgCl2 (Ambion, Cat. No. AM9530G), 10% N,N-dimethylformamide (Sigma ...
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bioRxiv - Immunology 2022Quote: ... on the ViiA 7 system (Applied Biosystems). PCR of CD19 was used for normalization ...
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bioRxiv - Immunology 2022Quote: ... on the ViiA 7 system (Applied Biosystems) as described (62 ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 µL HinFI (10U/µL, Thermo Scientific)) per 70 µL of cDNA and digested at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and a QuantStudio 7 Flex (Life Technologies) instrument ...
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bioRxiv - Neuroscience 2020Quote: ... with a ViiA 7 System (Applied BioSystems). The UBE2D2 gene transcript was used as the internal reference ...
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bioRxiv - Microbiology 2020Quote: ... and a QuantStudio 7 Flex (Life Technologies) instrument for Il17a (96) ...
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bioRxiv - Immunology 2022Quote: ... and 7-AAD (Invitrogen, 00-6993-50). CD19/CD20+ ...
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bioRxiv - Neuroscience 2022Quote: ... Potassium Chloride (Fisher Scientific- 7447-40-7) and Calcium chloride (Fisher Scientific- 10043-52-4).
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bioRxiv - Neuroscience 2022Quote: ... for 7 days in Neurobasal medium (Invitrogen) with 2% B-27 supplement (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... 7 mL of DMEM (Gibco, #11995-073) with 630 U/mL Type 1A collagenase from clostridium histolyticum (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR (Applied Biosystems, #ViiA 7) was performed using the KAPA SYBR FAST qPCR master Mix (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... CellEvent Caspase-3/7 Detection Reagent (Invitrogen) and Hoescht were added to the media at concentrations listed in S4 Table and incubated for 30min at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... CellEvent Caspase-3/7 (Life Technologies C10723), Fis1 polyclonal antibody (Proteintech 109561-AP) ...
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bioRxiv - Biochemistry 2020Quote: ... 7-hydroxycoumarin was obtained from Acros Organics, a part of Thermo Fisher Scientific (New Jersey ...
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bioRxiv - Cell Biology 2020Quote: ... on QuantStudio 7 Flex system (Life Technologies). To quantify viral genomes ...
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bioRxiv - Neuroscience 2021Quote: ... or QuantStudio 7 Pro SmartStart (Applied Biosystems) real-time PCR system ...