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Citations for Thermo Fisher :
2901 - 2950 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-conjugated beads were prepared by incubating antibody with beads in 300μL Blocking Buffer (PBS, 0.5% BSA [Invitrogen AM2616]) overnight at 4°C with rotation.
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bioRxiv - Immunology 2023Quote: ... All staining and blocking solutions were prepared in FACS buffer (a PBS solution with 5% fetal bovine serum (Gibco) and 0.1% sodium azide) ...
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bioRxiv - Cell Biology 2023Quote: ... The prepared samples were blocked in the blocking medium (PBS supplemented with 4% fetal bovine serum (Gibco #10500-064) and 1% bovine serum albumin (BSA)(Fisher Scientific #BPE9702-100) ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were washed again and 100 µL/well of Goat anti Rabbit IgG-HRP (1:9,000 in blocking buffer) (Invitrogen) was added and incubated for one hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Plates were removed from formalin and permeabilized with 0.2% Triton-X for 10 minutes at room temperature and treated with blocking buffer (Cell Stain Buffer; ThermoFisher). Infected cells were detected by consecutive incubation with CCHFV-specific antibody 9D5 (3 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... HUVEC samples were treated with an Fc receptor blocking reagent (1 test) (Thermo Fisher Scientific, Cat. # 14-9161-73) and incubated at 4°C for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... which involved a 30-minute incubation in a blocking buffer composed of 2.5% Normal Goat Serum (NGS) (ThermoFisher; 50062Z) and 2.5% Bovine Serum Albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue sections were then blocked with a 1x concentration blocking solution (Blocker™ BSA; #37525, ThermoFisher Scientific™) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer and developed using the SuperSignal West Femto Maximum Sensitivity Substrate kit (Thermofisher Scientific, Cat no. 34095). Membranes were imaged using FluorChem M Imager (ProteinSimple ...
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bioRxiv - Microbiology 2024Quote: ... for 10m and blocked overnight by using blocking solution (PBS plus 4% bovine serum albumin, fraction V, Fisher Scientific 9002-46-8 ...
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bioRxiv - Cancer Biology 2021Quote: Online chromatography of the extracted tryptic peptides was performed using an Ultimate 3000 HPLC system (Thermo Fisher Scientific) coupled online to a Q-Exactive-Plus mass spectrometer with a NanoFlex source (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The peptides were eluted with an acetonitrile gradient and electrosprayed into an Orbitrap Discovery mass spectrometer (Thermo Scientific) for analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Desalted peptides were labelled with TMTsixplex™ Isobaric Label Reagent Set (Catalog No. 90061, ThermoFisher Scientific, Waltham, MA) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... the peptides were loaded onto a C8 analytical column (1 mm i.d. × 50 mm length, Thermo Fisher Scientific) and separated with Agilent 1290 Infinity II UHPLC system ...
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bioRxiv - Cancer Biology 2022Quote: ... Desalted peptides were then labeled with 10-plex Tandem-mass-tag (TMT) reagents following manufacturer’s instructions (Thermo Scientific). The samples were then reconstituted in volume of 20 mM ammonium formate (pH 10 ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide mixtures were analyzed by LC-MS using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: About 500 ng of peptides were analysis using an Ultimate 3000 nanoflow liquid chromatography system (Thermo Scientific, USA) connected to a hybrid Q-Exactive HFX mass spectrometry (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The labeled sample was fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) and subject to liquid chromatography–MS/MS analysis using Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted peptides were introduced into Orbitrap Fusion Lumos system through a nanospray Flex™ ion source (Thermo Scientific) with the spray voltage of 2kV and a capillary temperature of 275°C ...
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bioRxiv - Molecular Biology 2021Quote: The peptide samples were analysed using a Lumos Orbitrap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific (Proxeon) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides eluted from the column were analysed on an Orbitrap Fusion Lumos (Thermo Fisher Scientific, San Jose, CA) mass spectrometer ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of desalted peptides were labelled immediately after being quantified with Qubit protein assay (Thermo Fisher Scientific). Multiplexed TMT samples were then fractionated using high-pH reverse phase chromatography ...
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bioRxiv - Biochemistry 2019Quote: Enriched peptides from the high pH reverse phase fractions were resolubilized in high purity water (ThermoFisher, Waltham, MA) with 0.1% formic acid (98.9% purity ...
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bioRxiv - Systems Biology 2020Quote: ... were resuspended in peptide loading buffer (0.1% FA, 0.03% TFA, 1% ACN) containing 0.2 pmol of isotopically labeled peptide calibrants (ThermoFisher 88321). Peptide mixtures were separated on a self-packed C18 (1.9 μm ...
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bioRxiv - Cell Biology 2019Quote: ... The extracted peptides were analyzed on an UltiMate 3000 RSLCnano/Q-Exactive system (Thermo Fisher Scientific, Bremen, Germany) that was set up with a Nanospray Flex ion source ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The collected peptide samples were analyzed by an Easy nLC Orbitrap Fusion Lumos platform (Thermo Fisher Scientific, USA). About 100 μg peptides were loading for the proteomic label free quantification ...
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bioRxiv - Plant Biology 2019Quote: ... The digested peptides were labeled with TMT10plex™ Isobaric Label Reagent Set (90309, Thermo Fisher Scientific, lot TE264412) and combined ...
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bioRxiv - Systems Biology 2019Quote: ... peptides were introduced by nano-electrospray into the mass spectrometer using an Easy-Spray source (Thermo Fisher Scientific) and data were acquired using MultiNotch synchronous precursor selection (SPS ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were prepared in house by mixing biotinylated (NANP)9 peptide with streptavidin conjugated PE or APC (Invitrogen) in a 4:1 molar ratio ...
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bioRxiv - Pathology 2020Quote: ... Protein and peptide concentrations were measured in duplicate using the Qubit® Protein Assay Kit (Invitrogen, Carlsbad, CA). Peptides were dried by vacuum centrifugation in 25 μg aliquots ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient with a flow rate of 300 nl min−1 ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75µm x 250 mm, 2µm particles; Thermo Scientific) at a flow rate of 300 µl/min using a 58-min gradient ...
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bioRxiv - Genetics 2019Quote: ... Preconcentration of peptides was performed on a precolumn (Pepmap RSLC, Thermo Scientific, C18, 100 µm x 2 cm) for 10 min at 20 µl/min flow (0.1% TFA ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peptides were concentrated using a trap column (0.075 × 20 mm, 3 µm, Acclaim PepMap 100 C18, Thermo Scientific) and then separated using a nano capillary column (0.1 × 150 mm ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: Peptides for each digested EV sample were separated in a Dionex Ultimate 3000 uHPLC (ThermoFisher Scientific, Paisley, UK) with an Acclaim™ PepMap™ 100 C18 trap column (3μm particle size ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting mRNA-peptide libraries (∼1013 hybrids) were adsorbed onto 200 µL of Oligo-dT cellulose (Fisher Scientific), washed with dT washing buffer (0.2 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: Peptides were analyzed on a Q-Exactive Orbitrap Mass Spectrometer attached to an Easy-nLC (Thermo Fisher Scientific) at 400 nL/min ...
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bioRxiv - Biochemistry 2021Quote: The separation of peptide mixture was performed using an Ultimate 3000 RSLCnano chromatographic HPLC system (Thermo Scientific, USA) connected to a Q-exactive HFX mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were loaded into an Acclaim PepMap 100 C18 trap column (0.2 mm × 20 mm, Thermo Fisher Scientific) with a flow rate of 5 μ l/min and separated on an EASY-Spray HPLC Column (75 μ m × 150 mm ...
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bioRxiv - Biophysics 2021Quote: ... Purified peptides were analyzed by mass spectrometry using a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: Eluting peptides were analyzed in a data-dependent acquisition mode on a Fusion Lumos mass spectrometer (ThermoFisher Scientific) coupled to the nano-HPLC (Dionex U3000 RSLCnano ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µl from each sample was saved for Pierce fluoremetric peptide quantification assay (Cat. No. 23290, Thermo Fisher), and 16 µl from each sample were combined to make an average reference sample for TMT batch normalization ...
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bioRxiv - Microbiology 2021Quote: Peptides (100 μg) of each sample were labeled with TMT reagent according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific). Desalted peptides were solubilized in 0.1% formic acid and 3% acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... The TMT-labeled peptide sample was subjected to LC-MS3 analysis using Orbitrap Fusion Lumos (Thermo Fisher Scientific) with the following mass spectrometric parameters ...
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bioRxiv - Cell Biology 2021Quote: ... The peptide was then coupled to the keyhole limpet hemocyanin according to the manufacturer’s protocol (Thermo-Fisher Scientific) and used to immunize rabbits (Cocalico Biologicals) ...
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bioRxiv - Microbiology 2021Quote: ... Extracted peptides were analysed by nanoLC-ESI-MS/MS using the Ultimate 3000/Orbitrap Fusion instrumentation (Thermo Scientific). The UniProt proteome for P ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were separated by reverse-phase chromatography on a 50cm EASY-Spray C18 LC column (Thermo Fisher Scientific) connected to an EASY-nLC 1200 instrument equipped with an autosampler (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Extracted peptides were analyzed by LC-MS/MS using a nanoflow HPLC system (Ultimate 3000 RSLC; ThermoFisher Scientific) coupled to an Orbitrap Fusion (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Lyophilized peptide aliquots of 400 µg (of starting protein) were labeled with TMT10-plex labeling kits (Thermo Fisher). Phosphopeptides were enriched with a 2-step enrichment process consisting of immunoprecipitation (IP ...