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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Pierce Reversible Protein Stain Kit for PVDF Membranes (including MemCode; cat# 24585; Thermo Fisher Scientific) was used to ensure equal loading ...
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bioRxiv - Molecular Biology 2023Quote: ... Pierce Reversible Protein Stain Kit for PVDF Membranes (including MemCode; cat# 24585; Thermo Fisher Scientific) was used to ensure equal loading.
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bioRxiv - Cell Biology 2023Quote: ... the membranes were imaged on a Chemi-Doc using the Pierce ECL kit (Thermo Fisher) for GAPDH and the SuperSignal Pico PLUS kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was extracted from frozen foetal membranes using PureLink Genomic DNA Mini kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: We evaluated the integrity of the neutrophil cell membrane as a metric for cell death using CyQUANT™ lactate dehydrogenase (LDH) cytotoxicity assay (Invitrogen #C20300), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Peroxidase activity was recorded using a commercially available kit (Amplex® Red Hydrogen Peroxide / Peroxidase Assay Kit, ThermoFisher Scientific). Samples were diluted 1:5 to bring values within the range of the kit standards ...
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bioRxiv - Genomics 2021Quote: ... DNA concentration was assessed with Qubit broad range kit (Qubit™ dsDNA BR Assay Kit, Q32850, Thermo Fisher Scientific) or nanodrop ...
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bioRxiv - Cancer Biology 2022Quote: ... following the instructions provided in the kit Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher). For DNA damage analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined with the MicroBCA Assay kit (Pierce BCA protein estimation kit, 23225, ThermoFisher Scientific, United Kingdom).
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bioRxiv - Microbiology 2024Quote: ... Sequencing cleanup was done using the QIAquick PCR Purification Kit followed by quantitation using the Qubit dsDNA HS Assay Kit on the Qubit 2.0 Fluorometer from Invitrogen by Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of the DNA was measured using a QUBIT kit 1x dsDNA HS Assay kit (ThermoFisher, Waltham, MA) and fragment size was assessed on a Femto Pulse (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts or purified sEVs were quantified for protein content using the bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific #23225). Equal amounts of cell lysate or purified sEVs were resolved by SDSPAGE and transferred into a polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2020Quote: ... Total protein concentration was assessed by bicinchoninic acid (BCA) assay using a Pierce BCA Protein Assay Kit accordingly to the manufacturer’s instructions (Thermo Scientific; Lenexa, KS, USA). Equal volumes of 30 μg total protein were denatured by adding Sample Loading Buffers (BIO-RAD Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... mRNA-LNPs were evaluated for encapsulation efficiency and mRNA concentration using RiboGreen assay using the Quant-iT RiboGreen RNA Assay Kit (Catalog # R11490, Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of whole cell lysates in assay buffer were used to quantify protein content using Reducing Agent-compatible Pierce BCA Assay Kit (Thermo Fisher Scientific #23250).
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bioRxiv - Plant Biology 2023Quote: ... were mixed with 100 μl of Y-PER reagent:2X β-Galactosidase Assay Buffer (1:1) using the Yeast β-Galactosidase Assay Kit (Thermo Scientific) in a 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... Total protein in BALF was determined by the bicinchoninic acid (BCA) colorimetric assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, #23227).
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were then purified by SPRI (Agentcourt, 1:1 sample: reagent ratio) and quantified with the Qubit fluorescence assay (Qubit dsDNA HS Assay Kit, ThermoFisher Scientific, MA, USA). Target-enriched RNA-seq libraries were analyzed on an Illumina NextSeq 500 sequencer using a NextSeq500/550 High Output v2 Kit (75 cycles ...
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bioRxiv - Developmental Biology 2023Quote: Cell cycle changes were additionally assessed using the 5-ethynyl-2’-deoxyuridine (EdU) assay using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10418). HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration from whole cell lysate was measured by BCA assay using Pierce™ BCA Protein Assay Kit (Cat# 23227, Thermo Scientific™) as per manufacturer protocol and 40 µg of protein per well was separated on 12% SDS PAGE ...
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bioRxiv - Microbiology 2024Quote: Compound/Virus-induced toxicity was measured by a colorimetric assay to detect lactate dehydrogenase (LDH) released into the basolateral medium using CyQUANT LDH Cytotoxicity Assay kit (Invitrogen, cat. no. C20300). In short ...
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bioRxiv - Evolutionary Biology 2024Quote: Further validations were also carried out for miR-277 and miR-34 with labelled microRNA pull down assays (LAMP) as well as expression level analyses (TaqMan™ MicroRNA Reverse Transcription Kit and TaqMan™ MicroRNA Assay (Applied Biosystems), Assay IDs are 464452_mat ...
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bioRxiv - Microbiology 2024Quote: Total protein was extracted by radioimmunoprecipitation assay lysis buffer and protein concentration was determined by a BCA Assay Kit (Thermo Fisher Scientific, USA). Equal protein amounts (60 μg ...
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bioRxiv - Cell Biology 2024Quote: ... the EdU assays were performed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10424), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Dried membranes (Nytran Supercharge Positively Charged Nylon Membrane (Fisher Scientific)) were blocked in 5% low-fat milk in PBS for 1h and incubated with primary antibody (anti-5mC ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the GeneJET Gel Extraction Kit (ThermoFisherScientific, Vilnius, Lithuania) and quantified using a fluorometric RiboGreen kit (Qubit Assay Kit, Invitrogen, Life Technologies Corporation, Oregon, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Generated DNA fragments were quantified with Qubit dsDNA HS Assay Kit (Invitrogen, Cat # Q3285). Libraries were pooled at equimolar concentration and submitted to WCM Genomics core ...
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bioRxiv - Zoology 2021Quote: ... DNAs were quantified fluorometrically by using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... DNA concentration was measured both by Qubit dsDNA HS assay kit (Thermo Fisher Scientific) and by qPCR using a KAPA Library Quantification Kit (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA concentration was estimated by Qubit RNA HS Assay Kit (Thermo Fisher Scientific, USA). The first strand cDNA was synthetized using ProtoScript II kit (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was adjusted using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For co-immunoprecipitation assay ...
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bioRxiv - Immunology 2021Quote: ... the concentration of each pool was measured by Qubit dsDNA HS Assay Kit (Invitrogen) and sizing was determined by Fragment Analyzer HS Small Fragment Kit (Agilent) ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries concentration was quantified with Qubit dsDNA HS (High Sensitivity) Assay Kit (Invitrogen, Q32854), and the final pool was generated by combining individual libraries in equimolar ratio ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), before extracts were stored at −80°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were estimated using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific# 23227). 400-500μg of protein from the stably transfected HEK293 cells were used to immunoprecipitate FLAG-tagged MIB1 using EZview™ Red ANTI-FLAG® M2 affinity gel (MilliporeSigma# F2426 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit (Thermofisher Scientific, 23225) colorimetric reaction and quantified on a SpectraMax i3 Multi-Mode Platform (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... Library concentrations were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher, Q32851) and pooled for sequencing ...
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bioRxiv - Immunology 2021Quote: ... which was measured using a Pierce Rapid Gold BCA Protein Assay kit (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Total protein concentration was assessed using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of whole-cell protein lysates samples were boiled in Laemmli loading buffer ...
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bioRxiv - Genetics 2021Quote: ... Shift assays were performed using the LightShift® Chemilumiescent EMSA Kit (Thermo Scientific 20148). DNA binding reactions were performed in a 20ul system containing biotinylated oligonucleotides and nuclear extracts (12ug) ...
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bioRxiv - Genetics 2021Quote: ... Protein amounts were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein (in vitro samples ...
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bioRxiv - Genetics 2021Quote: ... The resultant library was quantified using Qubit dsDNA HS Assay Kit (Q32854,ThermoFisher Scientific) and its quality was checked with Bioanalyzer using High Sensitivity DNA Kit (5067-4626 ...
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bioRxiv - Genetics 2021Quote: ... UV spectrophotometry and fluorometry (Qubit 4.0, using the Qubit dsDNA HS Assay Kit; Invitrogen) were used for the assessment of the quality and quantity of the isolated DNA ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined via the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Samples were quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA) and 1.2 mg of protein was used for Tandem Mass tags (TMT ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were quantified with Quant-iTTM PicoGreen® dsDNA Assay Kit (Thermo Fisher). Samples were combined in equal amounts (∼250 ng per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... EdU assays were completed with Click-iT Plus EdU Cell Proliferation Kit (Invitrogen, C10640) according to the manufacturer’s protocol.