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Citations for Thermo Fisher :
2901 - 2950 of 3282 citations for AKAP3 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... L-009522-01-00005) and non-silencing control siRNA (GEHealthcare, Dharmacon: D-001206-14-20) were transfected using Lipofectamine 2000 (Thermo Scientific) according to manufactureŕs protocol.
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bioRxiv - Cell Biology 2021Quote: Human PCNT siRNA (Smart Pool) (M-012172-01-0005; Dharmacon) was transfected into RPE1 cells with Lipofectamine RNAi MAX (13778100; ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... One day after seeding, BeWo cells were transfected with TRPV4 siRNAs (Horizonz, # MQ-004195-00-0002) using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen, # 13778075) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK 293 BRI3BP-HA cell line was transfected with control or BRI3BP silencer select siRNAs with Lipofectamine RNAi Max (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with the indicated siRNA construct (40 nM final concentration) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Specific knockdown of spartin in SUM159 cells was performed by transfection of small interfering RNA (siRNA) duplexes by Lipofectamine RNAiMAX (Life Technologies). The siRNAs for Negative Control (#D-001220-01 ...
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bioRxiv - Cell Biology 2021Quote: U2OS cells were seeded at appropriate density and transfected the following day with 10nM (unless otherwise specified) siRNA using Lipofectamine RNAiMax (Life Technologies) for 72 hrs ...
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bioRxiv - Systems Biology 2020Quote: 5637 cells were placed in 24 wells plate and transfected with the lentiviral vectors pTSB-CMV-puro and SiRNA against ACSS1 reaching 70%-80% confluence using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... with a final concentration of 10nM siRNA and 2uL/ml RNAiMAX reagent used according to the manufacturers’ instructions (Life Technologies, 13778030). The following siRNAs were used ...
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bioRxiv - Genomics 2021Quote: MIN6 cells between passages 17-21 were grown to approximately 80% confluency in 24-well plates and transfected with 30μM of either Socs1 siRNA (Invitrogen Silencer select) or scramble siRNA (Invitrogen Silencer Negative Control No ...
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bioRxiv - Microbiology 2021Quote: ... siRNA reverse transfection was performed by mixing 1.2 μl siRNA 5 μM with 1 μl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 μl with Opti-MEM® (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Dharmacon) and siGENOME Non-Targeting siRNA #2 (D-001210-02-05, Dharmacon) were transfected with Lipofectamine 3000 Reagent (L3000001, Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transfections of plasmids (Supplementary Table T8) or siRNA pools (15 nM; Supplementary Table T7) were carried out using Lipofectamine 2000 or Lipofectamine RNAiMax (Thermo Fisher), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... 2μL of siRNA was combined with 138μL of Opti-Mem Reduced Serum Medium with GlutaMax Supplement (Life Technologies, Cat #51985-034) and incubated at room temperature for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were seeded directly in 12-well plates at a density of 1.1x105 cells/well and were transfected on day 2 with 100 nM siRNA in Opti-MEM (Gibco 31985-070) using Lipofectamine RNAiMAX (Thermo Fisher 13778030 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were transiently transfected with small interfering RNA (siRNA, 50nM, Dharmacon, UK) using Lipofectamine RNAi MAX (Thermo Fisher Scientific, Paisley, UK). BCL-3 was knocked down using a single siRNA sequence ...
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bioRxiv - Cell Biology 2021Quote: ... For RNA interference, 20 nM Stealth siRNAs (Steath RNAi Negative Control Med GC Duplex #2, 12935112; LONP, HSS113887) (Thermo Fisher Scientific) or 5 nM Silencer select siRNAs (Silencer Select Negative Control #1 siRNA ...
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bioRxiv - Cell Biology 2021Quote: ... and were transfected with siRNAs at 50 nM for 48 h using the Lipofectamine™ RNAiMAX Reagent (13778-100, Invitrogen, USA).
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bioRxiv - Microbiology 2021Quote: ... were grown at ~75% confluency in 6 well plates and transfected with target- or negative control siRNAs (Table S2) using Lipofectamine 3000 as per the manufacturer’s (Invitrogen, Carlsbad, USA) instruction ...
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bioRxiv - Cell Biology 2022Quote: ... they were transfected with 0.006 pMol of siRNA (Dharmacon®) for each gene under study using RNAi Max Lipofectamine (Invitrogen®) (Supplementary Table S5) ...
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bioRxiv - Cell Biology 2022Quote: ... 300 pmol of each siRNA and 6 μg of each DNA was electroporated within the dedicated tip (Thermo Fisher Scientific, MPK10096). Electroporation parameters consisted in 4 pulses of 10 ms width and a voltage of 1230 V ...
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bioRxiv - Biochemistry 2022Quote: ... HURP or YFP only were transfected with either HURP or control siRNA (10 nM final concentration) using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen) in OPTIMEM medium (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... an shRNA-generating sequence equivalent to the above siRNA sequence was inserted into the pSilencer 4.1 CMV-Hygro vector (Ambion, Austin TX). The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml ...
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bioRxiv - Pathology 2022Quote: ... mRNA levels of target genes were knocked down through reverse transfection with SMARTpool silencing RNA (siRNA, Dharmacon) in Lipofectamine RNAiMAX transfection reagent (Thermo Fisher), used according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... M-100126-01-0005), rat Esrrg (Dharmacon, M-100212-01-0005), rat E2f1 (Dharmacon, M-102754-00-0005) or scramble control siRNA (ThermoFisher, 4390843), using the Viromer BLUE Transfection Reagent (Lipocalyx ...
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bioRxiv - Cell Biology 2022Quote: ... target sequence: GGACUAAGCCUGUAUGUGU) and Non-targeting siRNA (Dharmacon, D-001210-01-05) were transfected with Lipofectamine RNAiMAX Transfection Reagent (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 1µM of either control or CARP2 specific siRNA- sequence is − 5’ UGACAUCUCUACCGAAAUG 3’ using RNAimax (13778-075, Thermo Fisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Total RNA was isolated from drug treated or siRNA transfected HBEC 5637 cells using TRIzol reagent (Life Technologies, Grand Island, NY) and cDNA was synthesized ...
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bioRxiv - Microbiology 2021Quote: ... Cells were allowed to adhere for 4 hours (+/-1h) before transfection with individual siRNAs (10nM) diluted in a mix of OptiMEM (Life Technologies) and 0.05μL of Interferin reagent (Polyplus Transfection) ...
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bioRxiv - Neuroscience 2021Quote: ... RTN3 was knocked down in DRGN/retinal cultures using an siRNA to rat RTN3 (siRTN3; Assay ID 251374, cat no. AM16708, ThermoFisher Scientific). Protrudin (siProtrudin ...
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bioRxiv - Neuroscience 2020Quote: ... The non-coding control siRNA has been used previously and does not target any murine gene (ThermoFisher D-001206-14-05).
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bioRxiv - Microbiology 2021Quote: ... A549 cells in a 12-well format were transfected with 10 pmol of siRNA (Dharmacon; SMART pool against AJUBA, LIMD1 mRNA and a nonspecific control sequence) for 48 h using Lipofectamine RNAiMAX (Life Technologies). Plasmid transfections were performed according to manufacturer’s protocol in 12 -well plates or 4-well Lab Tek II chamber slides (Nunc ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Cells were transfected on two consecutive days with each siRNA (working concentration of 10nM) and 5μl Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... siRNA reverse transfection was performed by mixing 1.2 µl siRNA 5 µM with 1 µl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 µl with Opti-MEM® (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Three pmol of siNC or siAHDC1 stealth siRNA was incubated with 0.125 µl of Lipofectamine™ RNAiMAX Transfection Reagent (Cat. No. 13778030, Thermofisher scientific) in Opti-MEM™ I Reduced Serum Medium for 10 min and treated in each well ...
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bioRxiv - Cancer Biology 2022Quote: ... U2OS-TLR cells were transfected with 10nM final concentration of control or target siRNAs using Lipofectamine™ RNAiMAX (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... against Tmem135 (s91285) or in vivo negative control #1 siRNA (catalog 4459405) were complexed with Invivofectamine® 3.0 reagent (Life Technologies) according to manufacturer recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... VM-CUB1 and RT-112 cells were seeded at 1 × 105 cells per well in 6-well plates along with siRNA duplexes or miRNAs using Lipofectamine RNAiMAX Reagent (Life Technologies). At 72 hours after transfection ...
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bioRxiv - Physiology 2019Quote: ... cells were transfected with 10 nM of either silencer select Negative control No.2 (no. 4390847) or validated silencer select siRNA s15542 to target NR4A3 (Life Technologies). Transfections were performed in OptiMEM reduced serum media with Lipofectamine RNAiMAX transfection reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... BMDMs were seeded on six-well plates at a density of 5 × 105 cells/well overnight and then transfected with siRNA or the negative control at a final concentration of 100 nM using Lipofectamine 3000 (Invitrogen, USA). The interfering efficiency was detected by RT-qPCR or western blot 48 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... TC-32 and CADO-ES1 Ewing sarcoma cells were grown in 25 cm2 cell culture flasks (medium supplemented with 2% FBS) and transfected with indicated siRNA (25 pmol; cell density of 50%) using Lipofectamine RNAiMAX (Life Technologies). A set of pre-validated short interfering RNA (Silencer Select siRNA ...
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bioRxiv - Immunology 2020Quote: ... and 500nM of either BLIMP1 targeted siRNA (Silencer Select assay ID s1992) or scrambled control (Silencer Select Negative control, both from ThermoFisher Sceintific). Cells were electroporated using program U-015 ...
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bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Between 10–30 × 104 HUVECs were electroporated with 50 pmol of control or ArfGAP siRNAs using the Neon transfection system (Thermo Fisher) at 1350V for 30 ms ...
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bioRxiv - Cell Biology 2021Quote: ... 15 × 104 HEK 293 cells were seeded on collagen-coated coverslips (Cellmatrix, Type IV, Nitta gelatin, Osaka, Japan) and transfected with 5 nM siRNA using RNAiMax (Thermo Fisher) using a reverse-transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were first transfected with siRNA for 24 h and then transfected with different plasmids by Lipofectamine 2000 (Invitrogen, 11668019) again ...
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bioRxiv - Genetics 2020Quote: ... the medium was changed to serum-free NW media and siRNA treatment was performed overnight using the Lipofectamine RNAiMAX Transfection Reagent (Invitrogen, 13778150) protocol ...