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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... induction medium was replaced every 2-3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 μl/ml ...
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bioRxiv - Plant Biology 2023Quote: ... PI staining seedlings were washed for 2-3 min in water and transferred to a chambered cover glass (Thermo Scientific), and imaged either using Confocal laser scanning (CLSM ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed 3 times with TBS-T and 2 times in TBS and developed using SuperSignal West Pico PLUS Chemiluminescence reagent (ThermoFisher). The resulting membrane was imaged on a BioRad ChemiDoc system and visualized using Image Lab software (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... hESCs were differentiated into cardiac mesoderm by culturing for 3 days in RPMI B27-medium (RPMI 1640 GlutaMAX + 2% B27 supplement minus insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... induction medium was replaced every 2-3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 μl/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... MEFs and HeLa) or 2 µL of Lipofectamine 2000 (Calu-3 and VeroE6) according to the manufacturer’s recommendation (ThermoFisher and Invitrogen, respectively). For co-transfections ...
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bioRxiv - Bioengineering 2024Quote: ... and fresh media was added and incubated for a further 24 hours before performing the viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific). The media was replaced with fresh cell culture media containing MTT (0.25 mg/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... cell vials were thawed 2-3 min in a 37°C water bath and transferred in T25 cell culture flasks (Gibco) with 5ml of hASC culture medium containing Minimum Essential Mediun (αMEM ...
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bioRxiv - Genetics 2024Quote: ... The coverslips were then dried for 2-3 min and mounted on clean slides using Prolong Gold AntiFade (Life Technologies). Nikon confocal laser scanning microscope C2 TIRF was used to take images of the cells ...
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bioRxiv - Microbiology 2024Quote: ... the PBS or virus dilution in PBS was aspirated and 3 ml of overlay consisting of 1:1 2 x DMEM (DMEM high glucose, no sodium bicarbonate buffer powder [Gibco # 12-100-046] in 500 mL of RNase-free water ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: Duplex of the MEF2C enhancer sequence containing a mutation site [5′-ATGTATTTTTCTGCAATAAGT-3′ (×2)] in human genomic DNA were synthesized (Invitrogen). Additionally ...
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bioRxiv - Bioengineering 2024Quote: ... 2.08 x 102 ng/cm2 of pDNA was mixed with the 2 μL p3000 reagent per μg DNA then combined with 3 μL/μg Lipofectamine 3000 (Invitrogen) and incubated for 15 minutes before adding over cells ...
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bioRxiv - Immunology 2024Quote: ... the samples were rinsed with PBS 2-3 times and mounted with ProLong Gold antifade reagent containing DAPI (Life Technologies). The samples were then visualized using a Zeiss LSM 880 Confocal Laser Scanning Microscope ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated for 1 hour in 2-3 drops per slide of HRP-conjugated streptavidin (Alexa Fluor 594 Tyramide Superboost Kit, Invitrogen), washed 3 times for 10 minutes each in 1x PBS ...
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bioRxiv - Biochemistry 2024Quote: ... the final transfection mixture contained 3 μL of lipofectamine with 2 μg of total plasmid DNA in 200 μL OptiMEM (31985070, Gibco). The transfection mix was incubated for 20 min at room temperature before adding to seeded HEK cells (confluency ∼ 60-70% ...
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bioRxiv - Bioengineering 2024Quote: ... and primers (Eurofins Scientific, France) at the specified melting temperature (Tm) (Table 2) using a QuantStudio 3 (Thermo Fisher Scientific) thermocycler ...
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bioRxiv - Biochemistry 2024Quote: ... the degree of labelling was compared to hydrolysed succinimidyl 3-(2-pyridyldithio)propionate (SPDP, 0.3 kDa vs. 2.7 kDa, ThermoFisher, UK, #21857). To appraise the percentage labelling the fluorescent signals from the chased samples were calibrated against F-MAL standards ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorogenic probe 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (abbr. CPM, ThermoFisher, Cat# D346) in 100% DMSO was then added to both quench the enzymatic reaction and react with CoASH to yield the fluorescent CPM-SCoA complex for fluorescence quantification 27 ...
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bioRxiv - Neuroscience 2024Quote: The FM™ 1-43 (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) (Invitrogen) membrane probe was used to identify actively firing neurons and to investigate mechanisms of activity-dependent vesicle cycling ...
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bioRxiv - Biophysics 2020Quote: ... This initial PEG treatment was followed by a secondary PEG treatment with 25mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). These treatments resulted in a biotin-coated surface on the slide ...
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bioRxiv - Biophysics 2023Quote: ... then followed by a secondary PEG treatment with 25 mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). A microfluidics chamber was constructed on the slide ...
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bioRxiv - Biophysics 2024Quote: ... the slides were treated with a short-chain PEG solution (25 mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific) in 0.1 M sodium bicarbonate ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 2 mM MgCl2 supplemented with 2 μM Ultrapure Bovine Serum Albumin (Invitrogen) for 10 min at 25 °C ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Immunology 2021Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
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bioRxiv - Biochemistry 2020Quote: ... Cells were split at 1:2 every 2 days using Trypsin-EDTA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37 °C with 2 ul of FastDigest Mph1103I (ThermoFisher cat. no. FD0734) and 2 ul of FastDigest Eco105I (ThermoFisher cat ...
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bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37°C with 2 ul of FastDigest FspAI (ThermoFisher cat. no. FD1664) and 2 ul of FastDigest PmeI (ThermoFisher cat ...
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bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37 °C with 2 ul of FastDigest BamHI (ThermoFisher cat. no. FD0054) and 2 ul of FastDigest SbfI (ThermoFisher cat ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Immunology 2020Quote: ... then labelled with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluoresceinacetoxymethyl ester (Life Technologies, UK). Neutrophils were then added to wells under normoxia or hypoxia ...
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bioRxiv - Immunology 2020Quote: ... IL-2 was measured from culture supernatants using a mouse IL-2 ELISA kit (Thermo Scientific).
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bioRxiv - Genetics 2020Quote: 10T1/2: C3H10T1/2 cells were grown using the following media recipe: DMEM (ThermoFisher Scientific, 11965118), 10% FBS (ThermoFisher Scientific 26140079) ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μg of total RNA was treated with 2 units of DNase I (Thermo Fisher Scientific) and then used as a template for cDNA synthesis with RevertAid Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... in 2% TBS-T (1x Tris Buffered Saline, pH 7.6 with 2% Triton-X100, Fisher Scientific) for 1 h ...
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bioRxiv - Immunology 2024Quote: ... 25 mM N-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid (HEPES, Gibco, Grand Island, NY, USA), 100 U/mL penicillin and streptomycin (Gibco ...
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng purified DNA library was ligated with 2 μl barcode and 2 μl P1 (ThermoFisher). NGS analysis was performed using an Ion Torrent PGM (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mmol/L ethylenediaminetetraacetic acid) and stained with 2 μg/mL propidium iodide (PI; Invitrogen). PI was added just before the analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Equal volume of SYTOX Green (5 μM final in HBSS without Ca+2/Mg+2, Invitrogen) was added to each sample and incubated in the dark for 10 min ...
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bioRxiv - Systems Biology 2023Quote: ... We blocked cysteine residues using 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37 °C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA ...
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bioRxiv - Microbiology 2024Quote: ... heparin was biotinylated by mixing 2 mg heparin and 2 mg amine-PEG3-Biotin (Thermo Scientific), followed by the addition of 10 mg NaCNBH3 ...
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bioRxiv - Systems Biology 2024Quote: ... Cysteine residues were blocked by 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37°C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA ...
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bioRxiv - Bioengineering 2024Quote: ... EBMTM-2 basal media was supplemented with 2% exosome-depleted FBS (Thermo Fisher Scientific, Cat. # A2720803). Media was exchanged every 24-48 hours and cells were used between passages 3-6 ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... in neuronal differentiation medium (Neurobasal-A supplemented with 2% B-27, 2 mM L-glutamine (Gibco), 200 µM L-ascorbic acid 2-phosphate ...
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bioRxiv - Microbiology 2024Quote: ... Samples were washed with FACS buffer (PBS supplemented with 2% FBS and 2 mM EDTA (Gibco)) and were centrifuged at 400 g for 5 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... RNA (2 μg) from each sample was treated with TURBO™ DNase (2 Units, Thermo Scientific), and subjected to cDNA synthesis using iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: iPSC-derived neurons were incubated in 2.5 μM Fura-2-acetoxymethyl ester (Fura-2-AM, Invitrogen) for 50 min at 37°C ...