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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for Recombinant Mouse Map2k1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
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bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
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bioRxiv - Cell Biology 2020Quote: ... purified His-calmodulin was dialyzed in a Spectra/Por™ 4 RC Dialysis Membrane Tubing (08-667D, Thermo Fisher Scientific, Waltham, MA, USA) against 1,000 volumes of PBS supplemented with 0.5 mM EGTA at 4 °C for 4 h and replaced with fresh 1,000 volumes of PBS twice.
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
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bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
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bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
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bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
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bioRxiv - Plant Biology 2024Quote: ... to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD; ThermoFisher Scientific, Waltham, MA, USA) as described previously (Gampala et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Immunohistochemistry for mCherry (mouse, 1:1000, Takara; goat anti-mouse-alexa594, 1:500, Invitrogen) was used to delineate viral vector spread ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the indicated secondary antibodies: goat α-mouse 488 mouse (Invitrogen A11029), goat α-mouse 568 (Invitrogen A11004) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse GSCs and mouse NSCs were fixed with 1% formaldehyde (Thermo Fisher scientific, 28906) for 10 min and quenched with 0.125 M glycine for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-mouse IgG-Alexa 633 1:200 (mouse, A21052, Thermofisher, 1:200).
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bioRxiv - Bioengineering 2023Quote: ... an additional 45-min block was performed in mouse-on-mouse blocking reagent (Invitrogen). Sections were incubated with primary antibodies (Supplementary Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... and corresponding mouse mouse IgG1-PE isotype control (cat# 12-4714-42, ThermoFisher Scientific). Cells were washed twice in FW and analyzed by flow cytometry.
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bioRxiv - Physiology 2023Quote: ... A mouse anti-mouse antibody conjugated with Alexa Fluor 647 was used (Invitrogen A21058). 4′,6′-Diamidino-2-phenylindole staining was used to counterstain cellular nuclei ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse IL-10 and anti-mouse IL-13 antibodies were purchased from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-Actin (Invitrogen) at 1:3000 in 2% Bovine Serum Albumin (BSA) ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-PGK1 monoclonal (Invitrogen)) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-GAPDH (ThermoFisher AM4300), rabbit anti-Sra-1 (1/1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... or mouse IgG (ThermoFisher, 31903) were added to a spin column containing 20 ml of resin slurry in a 2-ml collection tube ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-V5 (Thermo Fisher Scientific Cat# R960-25 ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse 680 (Invitrogen), goat anti-rabbit 800 (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... or mouse (Invitrogen, 61-6520) secondary antibody conjugated to horseradish peroxidase (diluted 1:50 000 in TBS-T+ 5% non-fat milk ...
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bioRxiv - Microbiology 2021Quote: ... anti-mouse Alexa488 antibody (Invitrogen), and Hoechst 33258 (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2022Quote: ... goat anti-mouse (Invitrogen #G21040), goat anti-rabbit (Invitrogen #G21234).
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bioRxiv - Neuroscience 2021Quote: ... and mouse laminin (Thermo Fisher) as described previously (Venkatesh) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse Atxn3 (Thermofisher; Cat # Mm01336273), Beta Actin (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-V5 (Invitrogen R960-25 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-mouse Alexa 594 (Invitrogen), at 1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Thbs1 (Invitrogen, Mouse 1:100), Col1a2 (GeneTex ...
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bioRxiv - Cell Biology 2022Quote: ... mouse-anti V5 (Invitrogen, R96025). Donkey anti-rabbit Alexa 568 (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... Mouse CD106/VCAM1 PE (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... or mouse IgG (Fisher Scientific) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-GAPDH (Invitrogen, AM4300), mouse anti-β-actin (Proteintech ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse-HRP (1;10,000; Invitrogen), and anti-chicken-HRP (1:20,000 ...
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bioRxiv - Genetics 2020Quote: ... donkey anti-mouse (Invitrogen A21203), rabbit anti-guinea pig (ThermoFisher PA1-28595).
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-Golgin 97 (ThermoFisher), 1μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-acetylated-tubulin (Invitrogen) 1μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-mouse (Thermofisher, A11029) Alexa-fluor secondary antibodies.
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bioRxiv - Bioengineering 2020Quote: ... APC anti-mouse FoxP3 (Invitrogen), AF647 anti-mouse CD40 (BioLegend) ...
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bioRxiv - Bioengineering 2020Quote: ... AF532 anti-mouse CD8a (Invitrogen), PerCP-Cy5.5 anti-mouse CD45 (BioLegend) ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse GAPDH (Ambion; Am4300), anti-mouse HPV18 E6 (Arbor Vita Corporation ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-GAPDH (Ambion) at 1:3,000 dilution ...
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bioRxiv - Plant Biology 2020Quote: ... anti-mouse (ThermoFisher Scientific #A11001) or anti-rabbit (ThermoFisher Scientific #A11008 ...