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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Individual larval abdomens were placed in 2.0 mL microcentrifuge tubes along with 100mL of MagMAX PK enzyme mix (2mL of 100mg/mL Proteinase K and 98mL of PK buffer, both from Thermofisher) and a 3mm diameter steel bead ...
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bioRxiv - Cell Biology 2023Quote: ... Organoids were centrifuged and the pellet resuspended in 20μl of Proteinase K buffer from the Arcturus DNA PicoPure kit (ThermoFisher, KIT0103). They were incubated for 3 hours at 65°C followed by 10 minutes incubation at 95°C.
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bioRxiv - Neuroscience 2023Quote: ... A stock solution of proteinase K (PK) at a concentration of 20 mg/mL was obtained from Thermo Scientific (#EO0491). For PK digestions ...
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bioRxiv - Microbiology 2023Quote: ... 15 μl of 10% SDS (v/v) and 9 μl of PCR-grade proteinase K (20 mg/mL) (Thermo Fisher) for 30 min at 55°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Chemical composition of the sample surfaces was analyzed by X-ray photoelectron spectroscopy (XPS, Thermo Fisher Scientific K-Alpha XPS) with a 400 μm microfocused monochormatic Al Kα X-ray source ...
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bioRxiv - Immunology 2023Quote: ... each fraction (fraction 1-14) was collected separately and incubated with 20mg/ml Proteinase K (Thermo Scientific 25530049, 1:100), 0.5M EDTA and 20% SDS for 30 minutes at 42°C ...
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bioRxiv - Genomics 2023Quote: ... Samples were placed into separate PCR tubes containing 3 μl of lysis buffer with 0.3 mg/ml protein kinase K (Thermofisher, 26160). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... buffer was exchanged to 200 mM ammonium acetate (pH 4.5) with a Zeba™ 7 K MWCO desalting column (ThermoFisher). After buffer exchange ...
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bioRxiv - Bioengineering 2023Quote: ... Whole conditioned media from flasks and bioreactors was 0.2 μm filtered prior to first round UC at an RCFmax of 133,900 x g (Sorvall WX ultraCentrifuge, ThermoFisher; Fiberlite F37L-8×100 Fixed-Angle Rotor, ThermoFisher, k factor=168; PC Bottle Assembly 70 mL, ThermoFisher) for 1 hour at 4° C ...
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bioRxiv - Developmental Biology 2024Quote: ... These entry clones were recombined with the piggyBac vector42 (a gift from K. Woltjen) by using the Multisite Gateway technology (Invitrogen). The constructs were stably introduced into the human iPSCs and mouse EpiSCs by electroporation with a 4D Nucleofector (Lonza ...
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bioRxiv - Biochemistry 2024Quote: ... by transient transfection of pLV-EF1a-Prom1-Strep with VSVG and Delta 8.9 plasmids (a gift from K. Hochedlinger, Massachusetts General Hospital) using the Lipofectamine 3000 system (Thermo Fisher) and incubated overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in SYTOX Deep Red Nucleic Acid Stain dissolved in K-SFM (0.5 μM final concentration; ThermoFisher S11381) for 30 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... N/Tert-1 cells and all derived cell lines have been described previously and were maintained in keratinocyte-serum free medium (K-SFM; Invitrogen), supplemented with a 1% (vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected with a post-cre recombined version of the pSico plasmid and either mouse CIP4-mScarlet (Fig. 2d, e) or mouse FBP17-mScarlet (Fig. 2j, k) using Lipofectamine 3000 (Invitrogen) following the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and bound RNA oligonucleotides were released by proteolysis of FAST protein in 400 μl of SELEX Washing Buffer supplemented with 25 μg of Proteinase K (Invitrogen) for 1h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... the reactions were quenched by adding 10 µl proteinase digestion buffer (20 mM EDTA, 0.5 mg/ml Proteinase K (Invitrogen 100005393), 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... an aliquot of the reaction was quenched by addition of 500 mM EDTA and 20 μg Proteinase K (Thermo Scientific). Cas9 cleavage products using fluorescently labeled 55-bp linear fragments were resolved on a 15% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then washed in 1x PBS (5 min) and incubated in 1µg/mL Proteinase K (Thermo Fisher, Waltham, MA) at 37°C for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: N/Tert-1 cells and all derived cell lines have been described previously and were maintained in keratinocyte-serum free medium (K-SFM; Invitrogen), and supplemented with previously described antibiotics [27–29,88,92–95].
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bioRxiv - Cell Biology 2024Quote: For all cell biology experiments, mouse IMCD-3-FlpIn cells (gift from Peter K. Jackson, Stanford)79 were cultured in DMEM/F12 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... was added for 15 min at 37°C following addition of 0.1% SDS and 0.5 mg/ml Proteinase K (Thermo Fisher Scientific) for 15 min at 55°C ...
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bioRxiv - Cell Biology 2024Quote: The K-562 cell line was obtained from the ATCC collection (USA) and maintained in RPMI medium (#11875093, Gibco, USA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Blood was then removed from each tube and resuspended in ACK lysis buffer (Thermo Fisher) following the manufacturer’s instructions to lyse the blood cells ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was isolated from frozen parasite-infected blood pellets using a modified Trizol (Invitrogen) extraction protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... NSB-75 and peripheral blood B cells (PBBC) were cultured in RPMI+10% FBS (Gibco), supplemented with 2mM L-glutamine (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... mice peripheral blood from tail vein was lysed with 1× RBC lysis buffer (ThermoFisher Scientific) and stained with anti-human CD45 APC antibody (clone HI30 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted from 50 µl of blood serum using TRIzol LS (Thermo Fisher Scientific) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Neuroscience 2022Quote: ... Red blood cells were lysed using the ammonium chloride potassium (ACK) lysis buffer (Fisher Scientific) and centrifuged ...
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bioRxiv - Cancer Biology 2022Quote: ... red blood cell lysis was performed with RBC Lysis Buffer (Life Technologies, 00-4333-57).
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bioRxiv - Immunology 2020Quote: ... erythrocytes from 50 µL freshly drawn blood were lysed (ammonium-chloride-potassium lysing buffer [Gibco]), and cells were stained with Fixable Viability Dye (eBioscience ...
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bioRxiv - Microbiology 2020Quote: Whole blood (50 µL) was resuspended in 150 µL of TRIzol LS Reagent (Life Technologies). All Organs were added to Omni pre-filled bead tubes containing 1mL of TRIzol and homogenized using the OMNI bead ruptor 12 ...
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bioRxiv - Immunology 2020Quote: ... Blood-filled midguts were placed on 35 mm plastic dishes with glass bottom (Nunc, ThermoFisher). Dishes were mounted on inverted DMI6000 Leica Microscope ...
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bioRxiv - Immunology 2020Quote: ... Blood-filled midguts were placed on 35 mm plastic dishes with glass bottom (Nunc, ThermoFisher). Dishes were mounted on inverted DMI6000 Leica Microscope ...
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bioRxiv - Pathology 2021Quote: ... 100 µl cord blood was stimulated with pHrodo red-conjugated E.coli bioparticles (Phagocytosis kit, Thermofisher) at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... red blood cells were lysed by using the ACK lysis buffer (Thermo Fisher Scientific, A1049201). Cells were incubated at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... red blood cells lysed for 3min using 5cc of ACK lysis buffer (Thermo Fisher #A1049201) and then quenched with 20cc of FACS buffer ...
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bioRxiv - Pathology 2020Quote: ... a fraction of blood was mixed with the lysis/binding solution prepared in isopropanol (Thermofisher) and stored at −80°C for qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... and the SVF fraction was collected after red blood cell lysis (ACK Lysing Buffer, Gibco) and filtration through a 40 μm cell strainer.
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bioRxiv - Immunology 2021Quote: ... After lysis of red blood cells with ACK lysis buffer (Gibco Life Technologies, Waltham, MA), the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF ...
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bioRxiv - Microbiology 2020Quote: ... cells were subjected to red blood cell lysis with ACK lysis buffer (Gibco Catalog #A1049201), washed and counted using the Beckman Coulter Vi-CELL BLU.
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bioRxiv - Immunology 2021Quote: ... After lysis of red blood cells with ACK lysis buffer (Gibco Life Technologies, Waltham, MA), the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... Red blood cells were lysed in ammonium-chloride-potassium (ACK, Thermo Fisher Scientific, A10492-01) buffer and samples were washed in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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BTBD9 is a novel component of IGF signaling and regulates manganese-induced dopaminergic dysfunctionbioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from 250 μL of whole blood using TRIzol LS Reagent (Invitrogen). A total of 300 ng of RNA was used to obtain complementary DNA (cDNA ...
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bioRxiv - Microbiology 2021Quote: ... 90% N2 in a 2% suspension of human red blood cells in RPMI medium (Gibco) modified with the addition of 30 mM NaHCO3 ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA pools from human blood infections were globin-depleted using a GLOBINclear kit (Ambion) according to manufacturer’s specifications ...
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bioRxiv - Microbiology 2020Quote: Blood samples were collected in Greiner Bio-One Hematology K3EDTA Evacuated Tubes (Thermo Fisher Scientific). Complete blood counts ...
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bioRxiv - Immunology 2021Quote: ... and lysis of red blood cells using high yield lysing solution (Life Technologies, Carlsbad, CA). Cells were cryopreserved in 10% dimethyl-sulfoxide (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Red blood cells (RBC) from filtered cells were lysed in ACK lysis buffer (Life Technologies) for 5 min at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells (PBMC) and monocytes were cultured in RPMI 1640 (Invitrogen, Gaithersburg, MD) (R10 ...
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bioRxiv - Genomics 2020Quote: RT-PCR was done using RNA samples from peripheral blood (SuperScript III Reverse Transcriptase, Invitrogen). Specific cDNA primer pairs are (F ...