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Citations for Thermo Fisher :
2801 - 2850 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and used for cDNA synthesis with M-MLV Reverse Transcriptase (Invitrogen, #28025013). The transcript quantification of the target genes containing TISs of interest and Ubiquitin 366 (as an internal control ...
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bioRxiv - Neuroscience 2023Quote: Cell lysates were incubated with Dynabeads™ M-270 Epoxy (14301, Thermofisher) pre-coated with mouse anti-GFP (A11120 ...
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bioRxiv - Physiology 2024Quote: ... samples were embedded in Shandon M-1 Embedding Matrix (Thermo Fisher Scientific), snap-frozen and stored at −80°C until processing ...
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bioRxiv - Molecular Biology 2023Quote: (Per coupling reaction) 4 mg of M-270 Epoxy Dynabeads (Thermo Fisher) were equilibrated and washed in Epoxy A buffer (0.1 M sodium phosphate) ...
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bioRxiv - Microbiology 2023Quote: ... The lysate-RNA samples were incubated with Dynabeads M-280 Streptavidin (Invitrogen) on a shaker for 30 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was generated using total RNA and M-MLV reverse transcriptase (Invitrogen). qPCR was performed to measure 18s rRNA and WNV replicon RNA against a standard curve of a pooled reference to calculate relative RNA abundance.
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bioRxiv - Molecular Biology 2023Quote: ... and 1 µl RevertAid M-MuLV reverse transcriptase (200 U, ThermoFisher Scientific). Reaction mixtures were incubated at 25 °C for 10 min followed by incubation at 42 °C for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... in sterile filtered 0.02 M acetic acid (Thermo Fisher Scientific, Cat No.) and maintained at 37 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... and then reverse-transcribed to cDNA using M-MLV reverse transcriptase (Invitrogen). PCR was performed with a set of primers as listed in Supplementary Table 2.
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Evolutionary Adaptation of an HP1-protein Chromodomain Integrates Chromatin and DNA Sequence SignalsbioRxiv - Genetics 2024Quote: ... 0.15 M NaF using Zeba 7 kDa spin desalting columns (ThermoFisher Scientific) then diluted to approximately 50 µM in the desalting buffer ...
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed with the M-PER extraction reagent (Thermo Scientific, 78501) containing 1X Halt protease and phosphatase inhibitors (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: OPCs/pmOLs were lysed by using M-PER buffer (Thermo Scientific™) containing PhosSTOP™ Phosphatase Inhibitor Cocktail (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2019Quote: ... cfDNA in the plasma and CSF was then extracted using MagMAX™ Cell-Free DNA Isolation Kit (cat# 29319, ThermoFisher Scientific, Waltham, MA, USA) and stored at −20°C until use.
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bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were differentiated with GM-CSF (800U/ml) and IL-4 (500U/ml) in enriched RPMI (Gibco, 10% bovine serum, P/S, 2mM Glutamine) in a 5% CO2 humidified 37°C incubator as previously described (Geijtenbeek et al. ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Cell Biology 2021Quote: HEK293T (ATCC® CRL-3216™) and S1/S2 DKO HEK293 cells were maintained in cell culture in 1X DMEM (41965-039, Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321; Thermo Fisher Scientific, Waltham, MA) at a density of ∼0.5-2 x105 cells per well and grown to ∼80-90% confluence with standard media ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant single-strand AAV vectors were produced using the ultracentrifugation method described in a previous paper were produced using HEK293-T cells (HCL4517; Thermo Fisher Scientific; Waltham, MA), as described previously26 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488 chicken anti-rat IgG (Invitrogen Molecular probes Cat#A21470); and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488 chicken anti-rat IgG (Invitrogen Molecular probes Cat#A21470); and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... primary antibodies were visualized with Alexa 568–conjugated anti-rat antibodies (Invitrogen). Pictures were taken under 50x magnification ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin was visualized with rat anti-E-cadherin (Invitrogen #13-1900), both at 1:100 concentrations as per manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... antibodies diluted in PBS 0,1%Tween20 followed by α-rat 647 (Invitrogen) and α-rabbit AF488 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... goat anti-chicken IgY and goat anti-rat IgG secondary antibodies (Invitrogen) were used ...
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bioRxiv - Neuroscience 2022Quote: ... sections were stained with Alexa Fluor 647 goat anti-rat IgG (Invitrogen) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and goat anti-rat Alexa Fluor 488 (1:500; Molecular Probes (Invitrogen) Cat# A-11006 ...
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bioRxiv - Neuroscience 2021Quote: ... and goat anti-rat Alexa Fluor 488 (1:500; Molecular Probes (Invitrogen) Cat# A-11006 ...
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bioRxiv - Molecular Biology 2020Quote: ... and goat anti-rat lgG Alexa Fluor 633 (each 1:1000, Invitrogen). Samples were incubated in secondary antibodies for 1 day at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... rat monoclonal anti-LYVE1 (1:1000, Thermo Fisher Scientific, 14-0443-82), rabbit polyclonal anti-LYVE1 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and rat anti-mCherry antibody (M11217, Molecular probes, Thermo Scientific, 1:300). Secondary antibodies used are as following ...
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bioRxiv - Developmental Biology 2022Quote: ... and rat anti-mCherry antibody (M11217, Molecular probes, Thermo Scientific, 1:300). Secondary antibodies used are as following ...
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bioRxiv - Developmental Biology 2019Quote: ... goat-anti rat Alexa Fluor 555 (Molecular Probes, A-21434, 1:1000) and goat anti-rabbit Alexa Fluor 488 (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads sheep anti-rat IgG was purchased from Invitrogen (Carlsbad, CA, USA). VectaMount permanent mounting medium was used from Vector Laboratories (Burlingame ...
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bioRxiv - Developmental Biology 2019Quote: ... antibody and detected with an Alexa 568 goat α-rat (Life Technologies) secondary antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 594 goat anti-rat (1:1000, Invitrogen Molecular Probes, UK). Slices were mounted on slides ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 594 goat anti-rat (1:1000, Invitrogen Molecular Probes, UK). Slices were mounted on slides ...
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bioRxiv - Developmental Biology 2019Quote: ... Alexa Fluor® 546 Goat Anti-Rat IgG (H+L) (A11081, Invitrogen) 1:800/1:400 and Alexa Fluor® 647 Goat Anti-Rabbit (A21244 ...
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bioRxiv - Genetics 2020Quote: ... Secondary anti-rat antibody conjugated to Alexa568 or Alexa 647 (Life Technologies) was used to detect anti-CD105 ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA from rat DRG was reverse transcribed with Superscript II (Invitrogen) and then amplified by PCR using PrimeStar HS polymerase (Takara) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488 donkey anti-rat IgG (H+L) (Molecular Probes, A21208), Alexa Fluor 568 donkey anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... and Alexa-Fluor-647-labelled secondary anti-rat (Molecular Probes 1:400) antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... collagen I in 0.1% acetic acid (Collagen, rat tail, Type I, Invitrogen) was injected via the inlet of the cell-culture chamber ...
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bioRxiv - Bioengineering 2021Quote: ... except with a rat-specific collagen I primary antibody (PA1-36145, ThermoFisher) and a secondary antibody (A-11034 ...
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bioRxiv - Bioengineering 2020Quote: ... and donkey anti sheep 568 goat anti-rat secondary antibody (Thermo Fisher). The secondary antibodies were removed ...
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bioRxiv - Neuroscience 2021Quote: ... and biotinylated anti-mCD133 (rat monoclonal, 1:300, clone 13A4, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and biotinylated anti-mCD133 (rat monoclonal, 1:300, clone 13A4, Thermo Fisher Scientific ...