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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for GCLC Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Preprotech, cat#100- 18B) and 50 ng/mL recombinant sonic hedgehog (SHH, Preprotech, cat#100-45) in Knockout DMEM (Gibco cat #10829-018), 10% Knockout serum replacement (KOSR ...
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bioRxiv - Microbiology 2021Quote: ... ExpiSf9 cells for recombinant baculovirus production were cultured at 27°C and 125 rpm in ExpiSf CD Medium (Thermo Fisher Scientific, Waltham, MA). B19V-infected plasma sample was obtained from a donation center (CSL Behring AG ...
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bioRxiv - Biochemistry 2019Quote: ... A concentration range of indicated recombinant proteins was analyzed by thermal melt assays in SEC buffer using SYPRO Orange (Molecular Probes, cat.# S6651) using a Biorad CFX96 Touch Real-Time PCR C1000 System (ramping from 4 to 100°C at 1°C per minute).
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... were delivered into H9 cells along with recombinant True Cut Cas9 v2 using the Neon Transfection system (Thermo Fisher Scientific, Cat. No MPK5000). H9 cells (1×105 cells ...
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Genomics 2022Quote: ... The mini prep DNA was then used with the Bac-to-Bac™ expression system to generate a recombinant bacmid as per manufacturer conditions with MAX efficiency™ DH10Bac™ Competent Cells (ThermoFisher). After 4 hours of transformation recovery ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Microbiology 2023Quote: ... the binding of HIV+ plasma was detected using a biotin-tagged dimeric recombinant soluble FcγRIIIa (0.2 μg/mL) followed by the addition of Alexa Fluor 647-conjugated streptavidin (Thermo Fisher Scientific; 2 μg/mL). Infected cells were then stained intracellularly for HIV-1 p24 ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Biochemistry 2023Quote: ... The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific, Waltham, Massachusetts, USA). Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius ...
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bioRxiv - Cancer Biology 2022Quote: One million of BMDCs cultures in the absence or presence of 10 ng/ml of recombinant MDK (indicate provider and origin) were incubated with 1 μg/ml of Ovalbumin-AF594 conjugate (Thermo Fisher Scientific; O34781) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Immunology 2021Quote: ... Virus-infected cells were detected using in-house raised rabbit anti-S polyclonal antibody along with peroxidase-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Invitrogen). MN titers represented the reciprocal of the highest serum dilution that yielded >50% reduction in OD values as compared to wells containing virus only ...
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bioRxiv - Neuroscience 2020Quote: ... the brain slices were first washed and then stained for 120 minutes with the secondary antibody (Goat anti-Rabbit conjugated to AlexaFlour555, Invitrogen A21429, 1:500, Donkey anti-Rabbit conjugated to AlexaFlour488, Invitrogen A21206 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... The viral spike protein was stained with an in-house developed rabbit anti-spike antibody and goat anti-rabbit IgG (H+L) highly cross-adsorbed antibody with Alexa Fluor 488 (Invitrogen). Images were acquired using FluoView FV10i Confocal Laser Scanning Microscope (Olympus) ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody (goat anti-chicken 488 1:200, Invitrogen A32931; goat anti-rabbit 647 1:200, Invitrogen A21244; goat anti-rabbit 568 1:200, Invitrogen A11011 ...
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bioRxiv - Biochemistry 2020Quote: ... After washing three times in PBS, Alexa-Fluor antibodies (Donkey anti-Mouse 488, Goat anti-rabbit 488 and Donkey anti-rabbit 546 (Invitrogen) were added at a 1:200 dilution in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... GP was detected by western blotting with an anti-GP1 polyclonal rabbit serum described previously (22) followed by an Alexa-Fluor 680 dye conjugated anti-rabbit secondary antibody (ThermoFisher). Blots were imaged using the LI-COR Fc fluorescence imager (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated overnight at 4°C in a 5% milk solution containing a 1:1000 dilution of rabbit anti-DsbA antisera followed by incubation with a Goat anti-rabbit IgG HRP (ThermoFisher) secondary antibody at a dilution of 1:10,000 at room temperature for 30 minutes prior to development with a chemiluminescent kit (ThermoFisher).
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed the following morning and incubated for a minimum of 2h at RT with secondary antibody (rabbit Alexa Fluor-488 and rabbit Alexa Fluor-647, ThermoFisher). Finally ...
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bioRxiv - Microbiology 2020Quote: ... Cells are washed 5 times with 200 μL of PBS and incubated with 100 μL of the secondary antibody anti-rabbit (1/1000 in 2% BSA in PBS, Goat anti-Rabbit Alexa Fluor 647 #A21245 – Invitrogen) for 45 min at 4°C in a humidity chamber ...
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bioRxiv - Cell Biology 2021Quote: ... M2DCTs with an MyBP-C antibody (N-terminal rabbit polyclonal, 1:1000, kind gift from Samantha Harris) and anti-rabbit AlexaFluor secondary (ThermoFisher). Intercalated disk images were obtained by labeling fixed M2DCTs with an N-cadherin antibody (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... which was then blocked with 3% non-fat dry milk in PBS at room temperature for 1 h and incubated with primary antibodies (rabbit anti-pS404 Tau, CST-20194S; rabbit anti-pS262 Tau, Invitrogen-44-750G ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in PBS-Tween-20 containing 5% BSA for 1 h and incubated overnight at 4 °C with rabbit polyclonal anti-MIF antibody Ka565 (Bernhagen et al., 2007) or rabbit polyclonal anti-PF4V1 IgG PA5-21944 (Invitrogen) diluted in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Furin bands were detected using rabbit anti-furin polyclonal at 1:1000 and Goat anti-rabbit HRP (Invitrogen, 1:5000) was used as secondary antibody.
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor conjugated secondary antibodies (donkey-anti-rabbit Alexa-488, ThermoFisher Scientific, C22841, donkey-anti-rabbit Alexa-555, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30, 1:250, Invitrogen; Rabbit anti-MyosinVIIa ...
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bioRxiv - Microbiology 2019Quote: ... then incubated with rabbit antibodies to BspC or IgG at a 1:50 dilution followed by donkey anti-rabbit conjugated to AlexaFluor488 (Invitrogen). Bacteria were imaged using a BZ-X710 fluorescent microscope (Keyence).
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bioRxiv - Cell Biology 2019Quote: ... The parasites were subjected to incubation with secondary antibody (1:400 anti-rabbit Alexa 568, 1:400 anti-rabbit Alexa 488, 1:250 anti-mouse Alexa 594 from Invitrogen) for 1.5 hours followed by three washes with 1X PBS for 10 minutes each ...
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bioRxiv - Microbiology 2019Quote: ... labeled with anti-AgrAC rabbit antisera (Supplementary Fig. S3) and Cy5 goat anti-rabbit IgG secondary antibody (Life Technologies, CA), and visualized using a FluorChem Q Imager ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405, 1:1000, Abcam; donkey anti-rabbit 555, 1:1000, ThermoFisher). After four final washes ...
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bioRxiv - Pathology 2020Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (A11008) and Alexa Fluor 555 goat anti-rabbit IgG (A21428) were purchased from Invitrogen Biotechnology Co. ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
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bioRxiv - Genetics 2021Quote: ... We used an anti-Vasa antibody from Santa Cruz biologicals (anti-rabbit, 1:200) and a goat anti-rabbit secondary antibody from Invitrogen (Alexa Fluor Plus 488 1:500). We imaged ovaries on a Zeiss i880 confocal microscope with 405 and 488 nm laser lines at 40X (Plan-Apochromat 1.4 NA ...
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bioRxiv - Immunology 2021Quote: ... or fluorophore conjugated antibodies (Invitrogen, A32723, goat anti-mouse 488; Invitrogen, A21247, goat anti-rabbit 647; Invitrogen, A21206, goat anti-rabbit 488; Invitrogen, A21424 ...
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bioRxiv - Immunology 2021Quote: ... polyclonal rabbit anti-Banf1 (Cat. PA5-20329) and goat anti-rabbit IgG (H+L)-Alexa Fluor 647 were purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ZO-2 pAb (38-9100) and rabbit anti-ZO-3 pAb (36-4100) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... HA or GFP-tagged DISP1 variants (anti-HA rabbit polyclonal, 1:1000 or anti-GFP rabbit polyclonal, 1:2000, Invitrogen), and β-actin (anti-β-actin rabbit polyclonal ...