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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: Collected protein flow-throughs (FT) were subjected to protein precipitation for downstream protein content determination using bicinchoninic acid assay (BCA, Thermo Fisher) assay and trypsin protein digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
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bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
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bioRxiv - Neuroscience 2019Quote: ... Newly synthesized proteins were then enriched using the Click-it protein enrichment kit for chemistry capture of azide modified proteins (Invitrogen C10416) following the manufactures protocol with small modifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was performed according to Culturex protocol while protein purification was done using Pierce Protein A Agarose kit (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations across all samples were normalised to the same protein concentration (500 µg/ml) with protein determination quantified using the well-established Pierce BCA Protein Assay (Thermo Fisher) following the recommended guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
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bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
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bioRxiv - Developmental Biology 2024Quote: ... The purified proteins were stored at −80°C and the protein concentration was determined using Bradford plus protein assay reagents (23238, Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... and TAP-tagged proteins (TAP-Csm4, TAP-Mps2, and TAP-Mps3) were detected by an anti-TAP rabbit antibody (1:10,000, Thermo Fisher Scientific, cat#CAB1001). The level of Pgk1 was detected by a Pgk1 antibody (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... then permeabilized and stained with antibodies for intracellular protein for 1 h using the Foxp3 Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA). Data were collected in a FACSCalibur flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Bioengineering 2019Quote: The concentration of the monoclonal antibody in cell culture supernatant samples was determined by the Mab Pac protein A affinity column (Thermo scientific, CA, USA). Two buffer solutions were used for HPLC test ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection efficacy was confirmed by western-blot measurement of the expression of the HA-tagged fusion proteins with the HA tag monoclonal antibody (Thermo Fisher Scientific, USA).
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bioRxiv - Bioengineering 2020Quote: ... The anti-polyhistidine antibody that was used in polyhistidine-mediated S1 protein immobilization (see Figure S1) was purchased from Thermo Fisher (MA1-21315). The horseradish peroxide (HRP ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... containing primary antibodies (rabbit against tyrosine hydroxylase, 1:1000, AB152, Merck Millipore; and chicken against green-fluorescent protein, 1:750, A10262, Invitrogen, Thermo Fisher Scientific) to allow for sufficient target protein-primary antibody interaction ...
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bioRxiv - Genetics 2022Quote: ... After centrifugation 50µl of supernatant was saved as the total input and the rest was incubated with Swm antibody coated DynabeadsTM Protein G (Invitrogen, Cat. No. 10003D) for 2 hours at 4°C while rocking ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1D4 tagged MEGF8 was captured by a 1D4 antibody (The University of British Columbia) covalently conjugated to Protein A Dynabeads (Thermo Fisher Scientific, Invitrogen). Immunoprecipitates were washed once with IP Wash Buffer A (50 mM Tris at pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... the cell surface localized LAMP-1 protein was detected using immunolabeling with rat monoclonal anti-LAMP-1 antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific; RRID:AB_657536). The cells were moved to fresh media (containing chemical treatments ...
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bioRxiv - Immunology 2022Quote: PGT145 and VRC26-family antibodies were captured with Protein A columns (Cytiva, 11003493) and gently eluted with 3 M MgCl2 solution (Thermo Fisher Scientific, 21027). Elutions were then buffer exchanged to HEPES buffer (10 mM HEPES pH 8.0 and 75 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: For anti-Ty immunoprecipitations, Ty antibody (Bastin et al, 1996) was conjugated to agarose beads carrying protein G (Life Technologies cat. no. 22851) using 22 mM DMP ...
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bioRxiv - Biochemistry 2024Quote: ... single HEK293T cell clones exhibiting complete loss of endogenous PTER protein were confirmed via Western blotting using a polyclonal anti-PTER antibody (Invitrogen, TR-1003-G).
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Molecular Biology 2023Quote: Total protein lysates were immunoblotted onto nitrocellulose membranes and incubated with the following primary antibodies: anti-ELP3 (ThermoFisher, Cat.702669, 1:200 dilution), anti-eIF2α (Cell Signalling Cat.9722 ...
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bioRxiv - Molecular Biology 2024Quote: ... the bottom part (below 25 KDa according to the pre-stained protein ladder) was incubated with 1/1000 α-H2A.Z (39) and 1/10000 α-rabbit secondary HRP antibody (Thermo Fisher, NA934V).
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific), and extracts were loaded onto NuPAGE Bis–Tris SDS gels and immunoblots were visualized by LiCOR Odyssey system.
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), and the pHrodo-TDM1 fluorescent signal was normalized to the amount of protein at each time point.
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bioRxiv - Cancer Biology 2021Quote: ... The total protein concentrations were determined using BCA protein assay kit (Thermo Scientific), and IFN-α and IFN-γ protein concentrations were measured using a human IFN-α ELISA kit (1110012 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific) and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... protein concentration of the supernatant was determined using BCA protein assay (Thermo Fisher) and 50 μg (1C and S2F ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein content was quantified using a BCA Protein Assay Kit (Thermo Scientific) and equal amounts of total protein were loaded onto 4 – 15% Tris-Glycine gels (BioRad) ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were determined using the BCA protein assay (Thermo Fisher Scientific). Protein lysates were analyzed by SDS PAGE and Western blot using fluorescently-labeled secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using a reversible protein stain (Memcode, Pierce, Thermo Fisher Scientific) and membranes were incubated with the following primary antibodies listed in Table S3 ...
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bioRxiv - Cell Biology 2020Quote: ... The total protein content was measured using BCA Protein Assay Kit (ThermoFisher Scientific). The relative ALP activity was normalized to total protein content and expressed as units/g·protein.
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured using the Pierce BCA Protein Assay kit (Thermo Fisher). An equal volume of 2x Laemmli buffer (BIO-RAD ...
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bioRxiv - Cell Biology 2020Quote: ... and protein concentration was determined with the Pierce BCA protein assay kit (ThermoFisher). Insoluble materials were removed by centrifugation at 21,000 g for 30 mins at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). Aliquots of lysates were solubilized in Laemmli sample buffer and equal amounts of proteins were separated on 12% SDS-PAGE gels ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific). Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Sample protein concentrations were determined using the BCA Protein Assay Kit (Thermo Scientific). Primary antibodies were anti-Six1 (rabbit polyclonal HPA001893 ...