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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Expi293F cells were transfected with the pAdd2 5FnIII construct using the ExpiFectamine 293 kit (Thermo Fisher Scientific A14524) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was first packaged in 293 FT cells using co-transfection with LV-MAX Lentiviral packaging mix (ThermoFisher) for 48 hrs ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells (2.5×106 cells/ml) were transfected using ExpiFectamine™ 293 transfection reagent (ThermoFisher, cat. no. A14524). The cells were harvested 4-5 days post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293 cells were cultured in a 2L suspension using FreeStyle 293 Expression Medium (Life Technologies, Carlsbad, CA, USA) with a cell density maintained at 1 × 10^6 cells/mL on the day of transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293F cells were maintained in suspension within Freestyle™ 293 Expression Medium (#12338026, ThermoFisher Scientific, Waltham, MA, USA) at a temperature of 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: Human Embryonic Kidney Cells 293 (HEK293T cell) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Scientific™) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: Munc13 was expressed in ExpiHEK-293 cell cultures using ExpiFectamine as a transfection reagent (Thermo Fisher, Waltham, MA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Munc13C was expressed in ExpiHEK-293 cell cultures using ExpiFectamine as a transfection reagent (Thermo Fisher, Waltham, MA). Pellets were resuspended in lysis buffer and lysed using a Dounce homogenizer ...
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bioRxiv - Biochemistry 2023Quote: ... pcDNA5FRT-SNAP was co-transfected with pcDNA5FRT-Halo to the original Flp-In™-293 Cell Line (Invitrogen); and for positive control experiments ...
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bioRxiv - Systems Biology 2023Quote: ... 2.5× 106 cells were transfected with 10.8µg DNA using the ExpiFectamineTM 293 Transfection Kit (Thermo Fisher Scientific, A14525) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293S GnTI− cells (ATCC CRL-3022) were grown in suspension at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Immunology 2023Quote: ... plasmids were transfected into 293 T cell or Hela cell using Lipofectamine™ 3000 Transfection Reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Immunology 2024Quote: Recombinant rabbit mAbs and Fabs were expressed in HEK293F cells using ExpiFectamine™ 293 Transfection reagent (Gibco, A14525). Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse AlexaFluor®647 (1:1000 for Epifluorescence microscopy/1:100 for STORM Invitrogen), Chicken Cy5.5 (1:150 for STORM Jackson/Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... 1:4000) and Donkey anti mouse conjugated with Alexa Fluor 647 (Invitrogen, 1:4000). Cells were washed three times in 1xPBS with 0.1 % Tween 20 and once in 1xPBS ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-β-catenin (Abcam, 1:1000 mouse anti-N-cadherin (Invitrogen; 1:500), mouse anti-Glutamine Synthetase (GS ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200 ...
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bioRxiv - Cell Biology 2021Quote: ... Dylight 488 anti-mouse (1:1000, or 0.5 µg mL-1, ThermoFisher, Waltham, MA).
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bioRxiv - Cell Biology 2021Quote: ... Dylight 550 anti-mouse (1:1000, or 0.5 µg mL-1, ThermoFisher, Waltham, MA), Dylight 488 anti-mouse (1:1000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the coverslips were incubated 1 h with mouse anti–V5 antibody (Invitrogen, 1:500) and 1 h with the secondary antibody which was donkey anti–mouse coupled to Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... anti-H3 (Abcam ab1791, rabbit 1:4000 or Invitrogen #MA3-049, mouse 1:1000), anti-Lamin A/C (Santa Cruz sc-6215 ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with either anti-TIA-1 antibodies (mouse monoclonal, 1:200 dilution, Invitrogen), anti-G3BP1 (mouse monoclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... with the Mouse Monocyte Chemoattractant Protein-1/CCL2 (MCP-1) Uncoated ELISA Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-mouse (1:2000, #7076P2, Cell Signalling or 1:5000, #331430, Thermo Fisher) secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... or detected with a monoclonal mouse antibody (SantaCruz, sc-245, 1:250) and visualised with a donkey anti-mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1000). SCC1/ RAD21 was detected with a monoclonal mouse antibody (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (V/V) fetal bovine serum (HI FBS heat inactivated, Gibco) and GlutaMAX-I (Gibco)39 ...
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bioRxiv - Microbiology 2019Quote: ... 1x Pen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific), and pelleted at 350 xg for 5 min ...
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bioRxiv - Immunology 2021Quote: ... HI sera were tested using PrioCHECK PRRSV Antibody ELISA Kit (Thermo Fisher Scientific) for detection of PRRSV N protein-specific antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and anti-His tag (Alexa Fluor 555 conjugated, Thermo Fisher MA1-21315-A555) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... It was first amplified and inserted into pMT/V5-His B (ThermoFisher Scientific), using primers that introduced EcoRI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... Gβ1 and His-Gγ2 (C68S) were synthesised and cloned into pFastBac Dual (Invitrogen). For mammalian expression ...
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bioRxiv - Biochemistry 2020Quote: N-terminally His-tagged P-Rex2 was synthesised and cloned into pFastBac1 (Invitrogen). His-tagged PTEN ...
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bioRxiv - Molecular Biology 2019Quote: ... Results were detected using Western Blotting using anti-His primary antibody (ThermoFisher, His.H8).
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bioRxiv - Biophysics 2019Quote: ... dye free liposome was prepared with TexasRed conjugated anti-His tag Antibody (ThermoFisher) by mixing lipids with antibody containing buffer ...
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bioRxiv - Microbiology 2021Quote: ... and subcloned into expression vector pBAD/myc-His A (Invitrogen, Carlsbad, CA, USA) that produces a fusion six-histidine tag at the end ...
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bioRxiv - Biophysics 2021Quote: ... We used a rabbit anti-6X-His antibody (Invitrogen™, Waltham, MA, USA) to detect histidine-tagged proteins or mouse 1A9 antibody (GeneTex ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing was performed with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific) using Ion Proton instrument ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS- HI; Gibco) at 37 °C in a humidified 5% CO2 incubator ...
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bioRxiv - Cell Biology 2021Quote: The RNAP2 Ser2ph-mintbody sequence was inserted into pTrc-His (Thermo Fisher Scientific) to harbor the His-tag at the N-terminus ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using the Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher). Sequence reads were mapped to the hg19_AmpliSeq_Transcriptome_ERCC_v1.fasta reference ...
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bioRxiv - Microbiology 2020Quote: ... and His-PbChiB4 were purified with Ni-NTA agarose (Thermo Fisher Scientific, CAD) through native conditions following the manufacturer‘s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... PCR-mediated mutagenesis was performed using Phusion Hi Fidelity polymerase (Thermo Fisher Scientific) with the following thermocycler settings ...
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A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccinesbioRxiv - Cancer Biology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS, GIBCO, Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (10,000 U/mL ...
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A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccinesbioRxiv - Cancer Biology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS, GIBCO, Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (10,000 U/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Samples (2 µL) were suspended in 10 µL Hi-Di formamide (Thermo Fisher) and 0.5 µL MapMarker ROX 1000 (Eurogentec ...
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bioRxiv - Genetics 2020Quote: ... reactions were diluted with Novex Hi-Density TBE Sample Buffer (Thermo Fisher LC6678), and run on precast TBE gels ...
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bioRxiv - Molecular Biology 2022Quote: ... PfP2 sample was purified after binding with Hi-Bind Ni-NTA beads (Invitrogen) for 4h followed by elution of the sample with 20mM Tris-HCl (pH 7.4) ...