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Citations for Thermo Fisher :
2751 - 2800 of 6821 citations for Recombinant Human EFNA3 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Individual B cells were sorted into 96-wells PCR plates (Bioké) containing 2 μL lysis buffer (0.2% (v/v) Triton X-100 + 2U/μL RNaseOUT™ Recombinant Ribonuclease (Invitrogen) in UltraPure™ DNAse/RNase free distilled water (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... Purified recombinant HisHA-INF1 or HisHA-Scr74 were incubated with 20 μl Pierce Anti-HA magnetic beads (Thermo Fisher Scientific) at an amount of 500 pmol each for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... crude extracts and purified recombinant SepF and FtsZ in serial dilution were loaded on a 4−12 % (w/v) BisTris PAGE gel (Invitrogen). The amounts of crude extract were 120 µg for FtsZ and 290 µg for SepF ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNAs of NPH3SA and NPH3SE within the pENTR vectors were transferred to both pH35GS and pH35YG binary vectors (Yamaguchi et al., 2008) using an LR recombinant reaction (Invitrogen). 35Spro:NPH3SA ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... 59°C for 30s and 72°C for 30s using recombinant 5U/ul Taq polymerase prepared in 10X Buffer with 50mM MgCl2 (Invitrogen). The 388bp PCR amplicon was digested with 20U/µL EcoRV restriction enzyme prepared in 10X NEB 3.1 buffer (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Immunology 2022Quote: NK cells were enriched and incubated with 50 ng/ml recombinant mouse IL-15 (Preprotech) for four days in RPMI plus 10 % FBS with penicillin and streptomycin (Thermofisher) added in a U-bottom 96-well plate at the concentration of two million cells per ml ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids were transfected into Drosophila S2 cells using Calcium Phosphate Transfection Kit (Thermo Fisher Scientific, Paisley, Scotland, UK). Following antibiotic selection with Zeocin (InvivoGen ...
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bioRxiv - Microbiology 2020Quote: ... a recombinant bacmid DNA was generated from the sequencing-confirmed pFastBac construct using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... The bacmid was transfected into Sf9 cells to obtain recombinant baculovirus using Cellfectin II Reagent (Invitrogen Co., Carlsbad, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... fluorescent conjugate cholera toxin B (CTB; recombinant cholera toxin subunit B conjugated with Alexa fluorophores: 0.2% CTB-488 and CTB-594; Life Technologies) was injected into AL and PM.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
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bioRxiv - Microbiology 2020Quote: ... the macrophages were allowed to adhere to the culture plate for 20 minutes before 100 units (U) of murine recombinant interferon-gamma (IFNγ) (Invitrogen) was added to each well to stimulate the macrophages ...
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bioRxiv - Immunology 2020Quote: ... A total of 100.000 T cells were co-cultured with 50,000 sorted myeloid cells in the presence of 30IU recombinant hIL-2 plus anti-CD3/CD28 Dynabeads (ThermoFisher Scientific) for 3-4 days in round-bottom plates ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... These recombinant cells expressing individual rod opsin variants were then surface immunostained with a Dylight 550-labeled anti-HA antibody (ThermoFisher) and then fractionated according to the immunostaining intensity of expressed variants using FACS ...
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bioRxiv - Cancer Biology 2019Quote: ... The recombinant vector and miR-659 mimic were jointly transfected into MDA-MB-231 cells using Lipofectamine™2000 (Invitrogen). These cells were lysed after 24 h of culture and the activity of luciferase was determined.
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bioRxiv - Microbiology 2019Quote: ... Eight μL of Cellfectin II reagent and 3 ng of each recombinant plasmid were diluted in 100 μL Grace’s medium (Thermofisher Scientific), respectively ...
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bioRxiv - Microbiology 2019Quote: ... Stable cell lines expressing the recombinant protein were initially maintained in complete medium comprising of serum free medium Sf-900 II SFM (ThermoFisher) complemented with 10% v/v foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Microbiology 2019Quote: ... bacteria resuspended to OD660 0.3 were incubated with 1 µg of recombinant protein in the presence of 1x protease inhibitors (Pierce, Thermo Scientific) in sterile 1.5 ml microcentrifuge tubes with gentle end-over-end mixing for 30 min at 25°C.
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then activated by the addition of 0.2 μg/ml LPS and 0.1 ng/ml murine recombinant interferon-γ (IFNγ) (both ThermoFisher Scientific) for 24 h ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... for 3 hr at 37 °C with 10 μg/mL recombinant laminin-521 (BioLamina, LN521-05) diluted in PBS+/+ (Gibco). Single-cell suspensions were then incubated for 3 hr at 37 °C in HUESM medium supplemented with 20 ng/mL bFGF and 10 μM Rock inhibitor Y27632 ...
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bioRxiv - Biochemistry 2020Quote: ... in a donor vector (pDONR-221) and transferred by recombinant cloning into the glutathione S-transferase (GST) fusion vector pDEST15 (Invitrogen). The plasmid was used to transform Escherichia coli ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Immunology 2022Quote: ... supplemented RPMI (RPMI-1640 supplemented with 10% fetal bovine serum, penicillin-streptomycin, Sodium Pyruvate, MEM NEAA, 30U/ml recombinant IL2 (ThermoFisher) and 55 µM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: Recombinant baculovirus was produced using sf9 insect cells following the instructions of Bac-to-Bac TOPO Expression System (Invitrogen A11339). Suspended HEK293S GnTI- cells at the concentration of 3.5-4.0×106/mL in 37°C were infected with P2 virus ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
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bioRxiv - Zoology 2022Quote: ... Cardiac muscle was detected with a rabbit recombinant polyclonal primary antibody to cardiac troponin I (cTnI, Invitrogen, 1HCLC, 1:600) and a donkey anti-rabbit antibody conjugated to the fluorophore Alexa 647 nm (Invitrogen A31573 ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Neuroscience 2024Quote: ... Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 488/555/647 Conjugate (CTB-488/555/647, 0.1% wt/vol, 80 nL, ThermoFisher C34775/C34776/C34778 ...
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bioRxiv - Molecular Biology 2023Quote: Introduction of recombinant genes into the parental HEKR4 cell line was performed according to the manufacturer’s guidelines (Thermo Fisher Scientific). In brief ...
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bioRxiv - Systems Biology 2023Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant pCS2+ plasmids containing the ORs were purified using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). The plasmids were linearized by NotI (Promega ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Cell Biology 2023Quote: Pooled HUVEC sample sets were grown to confluence before treatment with or without recombinant tumour necrosis factor alpha (TNF; 10 ng/mL) (ThermoFisher) in cell culture medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant bacmid DNA that carried the mini-HA domain was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...