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• NOTUM-MEDIATED WNT SILENCING DRIVES EXTRAVILLOUS TROPHOBLAST CELL LINEAGE DEVELOPMENT

  Vinay Shukla, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Alexa Fluor 568 goat anti-mouse IgG (1:800, A11031, Thermo Fisher), Alexa Fluor 568 goat anti-rabbit IgG ...
• NOTUM-MEDIATED WNT SILENCING DRIVES EXTRAVILLOUS TROPHOBLAST CELL LINEAGE DEVELOPMENT

  Vinay Shukla, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Alexa Fluor 568 goat anti-mouse IgG (1:800, A11031, Thermo Fisher), Alexa Fluor 568 goat anti-rabbit IgG ...
• Proteostatic remodeling of small heat shock chaperones - crystallins by Ran-binding protein 2 and the peptidyl-prolyl cis-trans isomerase and chaperone activities of its cyclophilin domain

  Hemangi Patil, Kyoung-in Cho, Paulo A. Ferreira, bioRxiv - Genetics 2024

  Quote: ... mouse anti-mHsp70 (1:600; Thermo Scientific, Rockford, IL; Cat: MA3–028), rabbit anti-COUP-TF1 [1:1,000 (IB) ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... and Alexa Fluor 488 goat anti-mouse IgG (1:500, A11029, Invitrogen).
• DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks

  Anja Göder, et al., bioRxiv - Biochemistry 2023

  Quote: ... and Goat anti-mouse IgG1 Alexa Fluor 546 (A21123, ThermoFisher; 1:300) to detect incorporated IdU ...
• A ribosome-associating chaperone mediates GTP-driven vectorial folding of nascent eEF1A

  Ibrahim M. Sabbarini, et al., bioRxiv - Biochemistry 2024

  Quote: ... mouse-anti-Pgk1 ([1:2000 - IB], 459250, Thermo Fisher Scientific, Waltham, MA), mouse-anti-Myc 9E10 ([1:10,000 - IB] ...
• An Immunological Synapse Formation Between T Regulatory Cells and Cancer-Associated Fibroblasts Promotes Tumor Development

  Athina Varveri, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and secondary antibodies: Alexa Fluor 555 anti-mouse IgG (1:500; Invitrogen), Alexa Fluor 647 anti-rabbit IgG (1:200 ...
• On growth and form of the mammary gland: Mesenchyme instructs growth while epithelium directs branching

  Qiang Lan, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-mouse Krt14 (RB-9020-P, Thermo Fisher Scientific, 1:500), rat anti-Krt8 (TROMA-1 ...
• Generation of human cerebral organoids with a structured outer subventricular zone

  Ryan Walsh, et al., bioRxiv - Developmental Biology 2023

  Quote: ... donkey anti- mouse Alexa Fluor 488 (Thermo Fisher, A-21202, 1:1000), donkey anti-rabbit Alexa Fluor 555 (Thermo Fisher ...
• Drosophila FMRP recruits the miRISC to target mRNAs to repress translation

  Navneeta Kaul, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and an anti-mouse Alexa 488-conjugaged secondary (1:1000, Molecular Probes). Preps were then mounted on charged slides in DAPI Flourmount G (Southern Biotech ...
• A shift in the mechanisms controlling hippocampal engram formation during brain maturation

  Adam I. Ramsaran, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-mouse Alexa Fluor 647 (1:500, Invitrogen, cat# A-21244), goat anti-mouse Alexa Fluor 488 (1:500 ...
• Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments

  Yuki Ogawa, et al., bioRxiv - Neuroscience 2023

  Quote: ... Alexa Fluor 594 anti-mouse IgG (1:1000 Thermo Fisher Scientific A32742). Streptavidin Alexa Fluor 594 conjugates were purchased from Thermo Fisher Scientific (1:5000 ...
• Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments

  Yuki Ogawa, et al., bioRxiv - Neuroscience 2023

  Quote: ... Alexa Fluor 488 anti-mouse IgG (1:1000 Thermo Fisher Scientific A11029), Alexa Fluor 594 anti-rabbit (1:1000 Thermo Fisher Scientific A11034) ...
• Axonal ER tubules regulate local translation via P180/RRBP1-mediated ribosome interactions

  Max Koppers, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-mouse Alexa568 (Thermo Fisher; Cat#A-11031, RRID:AB_144696, 1:1000), goat anti-mouse Alexa405 (Molecular Probes ...
• Shedding light on human olfaction: electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

  Andres Hernandez-Clavijo, et al., bioRxiv - Neuroscience 2023

  Quote: ... donkey anti-mouse Alexa Fluor 594 (1:500, A-21203, Life Technologies), donkey anti-mouse Alexa Fluor 488 (1:500 ...
• Motile Living Biobots Self-Construct from Adult Human Somatic Progenitor Seed Cells

  Gizem Gumuskaya, et al., bioRxiv - Bioengineering 2023

  Quote: ... Alexa Fluor 594-conjugated mouse anti-ZO-1 (Thermo Fisher Scientific #339194). Images were collected using a Lecia SP8 FLIM microscope with a 25× water immersion objective ...
• DNA Combing versus DNA Spreading and the Separation of Sister Chromatids

  Alice Meroni, et al., bioRxiv - Molecular Biology 2023

  Quote: ... incubated with anti-mouse Alexa Fluor 647 (1/100, A28181, ThermoFisher Scientific) for 45 min at R.T. ...
• Enhancing glymphatic function with very low-intensity ultrasound via the transient receptor potential vanilloid-4-aquaporin-4 pathway

  Wei-Hao Liao, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse anti-GFAP (Cat No. MA5-12023, 1:500, Thermo Fisher Scientific) and mouse anti-AQP4 (Cat No ...
• Regulation of oncogene-induced senescence by the MRE11 and TREX1 nucleases

  Hervé Técher, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Goat anti-mouse IgG1Alexa 546 (1/100 PBS/T, Molecular Probes, A21123). The ssDNA is detected with Anti-ssDNA (1/100 ...
• The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

  Mizuki Morita, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 1:500 anti-mouse IgG-Alexa 647-conjugated donkey antibody (Invitrogen A31571), and 1:500 anti-goat IgG-Alexa 488-conjugated donkey antibody (Invitrogen A11055 ...
• Chondroitin and dermatan sulfate exposure induces a wound healing state in fibroblasts through Cux1-mediated SerpinB2 transcriptional repression

  Alba Diaz-Pizarro, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Secondary antibodies were Alexa Fluor 488 anti-mouse (1:500, Invitrogen #A21202) and Alexa Fluor 594 anti-goat (1:500 ...
• The AAA+ protein Msp1 selects substrates by recognizing a hydrophobic mismatch between the substrate transmembrane domain and the lipid bilayer

  Heidi L. Fresenius, et al., bioRxiv - Biochemistry 2023

  Quote: ... and 1:10,000 dilution of goat anti-mouse-HRP secondary antibody (Invitrogen).
• Nuclear MTHFD2 secures mitosis progression by preserving centromere integrity

  Natalia Pardo-Lorente, et al., bioRxiv - Cell Biology 2023

  Quote: ... and goat-anti mouse 555 (Thermo Fisher Scientific; #A-28180; 1:1000). Nuclei were visualized with DAPI (Biogen Cientifica ...
• Porous membrane electrical cell-substrate impedance spectroscopy for versatile assessment of biological barriers in vitro

  Oleg Chebotarev, et al., bioRxiv - Bioengineering 2023

  Quote: ... Primary antibodies were mouse monoclonal ZO-1 (Thermo Fisher Scientific, 33-9100) and rabbit polyclonal GFAP (ab7260 ...
• The components of an electrical synapse as revealed by expansion microscopy of a single synaptic contact

  Sandra P. Cárdenas-García, et al., bioRxiv - Neuroscience 2023

  Quote: ... combinations of mouse IgG-Alexa Fluor 546 (Invitrogen, cat. A11030, 1:200), mouse IgG-Alexa Fluor 647 (Invitrogen ...
• A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and asymptomatic bacteria

  Carlos Flores, et al., bioRxiv - Microbiology 2023

  Quote: ... or 1:30 for mouse anti-uroplakin-III (UPIII) monoclonal antibody (Invitrogen). After being washed three times in 1% BSA/PBS ...
• A REM-active basal ganglia circuit that regulates anxiety

  Wei Ba, et al., bioRxiv - Neuroscience 2023

  Quote: ... Alexa Fluor 594 goat anti-mouse (1:1000, Invitrogen Molecular Probes, UK).
• Mesothelial cells exhibit characteristics of perivascular cells in an in vitro angiogenesis assay

  Chrysa Koukorava, et al., bioRxiv - Cell Biology 2023

  Quote: ... including optimized mouse primers (Supplementary Table 1) on a ViiA7 (Thermofisher/ABI). Cycles consisted of an initial incubation at 50° C and 95° C for 2 and 10 minutes respectively ...
• Serotonergic reinforcement of a complete swallowing circuit

  Andreas Schoofs, et al., bioRxiv - Neuroscience 2023

  Quote: ... or anti-mouse Alexa Fluor 633 (1:500, goat, Invitrogen, A-21052). For occasional F-actin staining ...
• Transcriptomic comparison of two selective retinal cell ablation paradigms in zebrafish reveals shared and cell-specific regenerative responses

  Kevin Emmerich, et al., bioRxiv - Neuroscience 2023

  Quote: ... Secondary antibodies included: anti-mouse Alexa Fluor 430 (1:500; Life Technologies), anti-mouse Alexa Fluor 635 (1:500 ...
• Targeting Ras signaling excitability in cancer cells through combined inhibition of FAK and PI3K

  Chao-Cheng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Donkey anti-Mouse Alexa FluorTM 647 (ThermoFisher, A-31571, 1:5000).
• Nuclear MTHFD2 secures mitosis progression by preserving centromere integrity

  Natalia Pardo-Lorente, et al., bioRxiv - Cell Biology 2023

  Quote: ... Alexa Fluor 555 goat anti-mouse (Thermo Fisher Scientific; #A21058; 1:1000) and Alexa Fluor 647 goat anti-human (Thermo Fisher Scientific ...
• The proteomic landscape of genotoxic stress-induced micronuclei

  Kate M. MacDonald, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1:500 AlexaFluor 568 Goat anti-mouse IgG secondary (Invitrogen cat #A11004); 1:500 AlexaFluor 488 Goat anti-rabbit IgG secondary (Invitrogen cat #A11034) ...
• The proteomic landscape of genotoxic stress-induced micronuclei

  Kate M. MacDonald, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1:500 AlexaFluor 488 Goat anti-mouse IgG secondary (Invitrogen cat #A11001); 1:500 AlexaFluor 568 Goat anti-mouse IgG secondary (Invitrogen cat #A11004) ...
• Chemogenetic profiling of ubiquitin-like modifier pathways identifies NFATC2IP as a mediator of SUMO-dependent genome integrity

  Tiffany Cho, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Alexa Fluor 647 goat anti-mouse IgG (Invitrogen, cat # A21236, 1:1000), and Alexa Fluor 488 goat anti-human IgG (H+L ...
• TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic pain

  Kathryn A. Swanson, et al., bioRxiv - Neuroscience 2023

  Quote: ... and AlexaFluor488 (goat anti-mouse, 1:500, Thermo Fisher Scientific A-11029). Sections were washed ...
• Mettl1-dependent m⁷G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster

  Shunya Kaneko, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Alexa 488-conjugated anti-mouse IgG (Thermo Fisher, A11029, 1:250 dilution), and Alexa 546-conjugated anti-rabbit IgG (Thermo Fisher ...
• Dysregulated actin dynamics and cofilin correlate with TDP-43 pathology in sporadic amyotrophic lateral sclerosis

  Cyril Jones Jagaraj, et al., bioRxiv - Neuroscience 2023

  Quote: ... and rabbit anti-mouse Alexa Fluor 488 (Invitrogen, #A-11059, 1:200) for 1hr in the dark at RT ...
• Spatially distinct inputs modulate the amount of active Mitotic-phase GAP to locally restrict RhoA signaling for successful cell division

  Friederike Wolff, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Alexa FluorTM 635 goat anti-mouse (1:500, A31574, Life Technologies), and Hoechst 33258 (861405 ...
• mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy

  Lisa Maria Riedmayr, et al., bioRxiv - Bioengineering 2023

  Quote: ... and Alexa Fluor Plus 488 anti-mouse (1:800, Thermo Fisher Scientific). No antibodies were used on FHC sections ...
• Control of atypical PKCι membrane dissociation by tyrosine phosphorylation within a PB1-C1 interdomain interface

  Mathias Cobbaut, et al., bioRxiv - Biochemistry 2023

  Quote: ... Secondary antibody was 1:1500 goat anti-mouse 488 (Thermo Fisher, A11001). Coverslips were mounted using Prolong gold with DAPI (Thermo Fisher Scientific ...
• Human IAPP is a contributor to painful diabetic peripheral neuropathy

  Mohammed M. H. Albariqi, et al., bioRxiv - Neuroscience 2023

  Quote: ... and Alexa Fluor 568 labeled donkey anti-mouse (1:1000, Life Technologies) in the dark for 1 hour ...
• Chronic exposure to odors at naturally occurring concentrations triggers limited plasticity in early stages of Drosophila olfactory processing

  Zhannetta V. Gugel, et al., bioRxiv - Neuroscience 2022

  Quote: ... and Alexa Fluor 633 goat anti-mouse (1:500, Thermo Fisher #A21050) secondary antibodies ...
• Transcriptional networks are dynamically regulated during cell cycle progression in human Pluripotent Stem Cells

  Anna Osnato, Stephanie Brown, Ludovic Vallier, bioRxiv - Cell Biology 2022

  Quote: ... Alexa Fluor® 647 goat anti-mouse IgM (1:1,000, Thermo Fisher). Cells were washed with PBS ...
• A Novel Transcription Factor Combination for Direct Reprogramming to a Spontaneously Contracting Human Cardiomyocyte-like State

  Marisol Romero-Tejeda, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Alexa Fluor 594 Goat anti Mouse IgG1(1:500, Invitrogen, A21125) in 1 mg/mL BSA plus 0.1% Tween (Fisher ...
• The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

  Christoph Wenger, et al., bioRxiv - Biochemistry 2022

  Quote: ... goat anti-mouse Alexa Fluor 596 (ThermoFisher Scientific, dilution IF 1:1’000), goat anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific ...
• A murine model of hnRNPH2-related neurodevelopmental disorder recapitulates clinical features of human disease and reveals a mechanism for genetic compensation of HNRNPH2

  Ane Korff, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1 µg/ml Natural Mouse Laminin (Thermo Fisher Scientific, Waltham, MA, USA), 1uM AraC (Sigma Aldrich ...
• Ribonucleotide synthesis by NME6 fuels mitochondrial gene expression

  Nils Grotehans, et al., bioRxiv - Cell Biology 2022

  Quote: ... and anti-mouse IgM-Alexa 568 nm (Thermo Fisher Scientific; 1:1000) antibodies in 40 µL PBS for 30 min at room temperature ...
• A hierarchical pathway for assembly of the distal appendages that organize primary cilia

  Tomoharu Kanie, et al., bioRxiv - Cell Biology 2023

  Quote: ... goat anti-mouse IgG2b Alexa Fluor 647 (A-21242, Invitrogen, 1:1000), goat anti-mouse IgG2a CF568 (20258 ...
• Downregulation of stromal syntenin sustains AML development

  Raphael Leblanc, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and HRP-conjugated secondary antibodies (Mouse or Rabbit, Thermofisher scientific; 1/10000). Signals were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare).