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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... was validated by STR analysis using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Cat n:4486467, ThermoFisher Scientific) using the provided protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Proliferation was assessed using a Click-iT EdU Alexa Fluor 594 Imaging Kit (ThermoFisher; n=3/group/time point). EdU was added to media at a concentration of 10 µM at days 2 and 6 of culture and incubated for 24 hours before gels were fixed at days 3 and 7 ...
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bioRxiv - Biochemistry 2021Quote: ... the Lambda DNA in TE from Quant-iT ™ PicoGreen® dsDNA Assay Kit (ThermoFisher Scientific, cat. n. P11496) was used ...
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bioRxiv - Biochemistry 2021Quote: ... H3.3 was cloned into pLEX_305-C-dTAG and p53 as well as RelA were cloned into pLEX_305-N-dTAG using Gateway LR clonase II Enzyme mix kit (Invitrogen). The sequences of the primers are listed below.
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA of all ancestral (A) and evolved (N) lines were isolated using Yeast DNA Extraction kit (Thermo Scientific) following manufacturer’s protocol ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... and was quantified using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies, Grand Island,N NY, USA) and Epoch Microplate Spectrophotometer (BioTek Instruments) ...
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bioRxiv - Cell Biology 2024Quote: ... the primary amine group of N-WASP was labeled using an Alexa Fluor™ 488 labeling kit (Thermo Scientific). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting lysate was used to quantify the amount of clusterin present in each sample using the Mouse Clusterin ELISA Kit (Thermo Fisher EM18RB) according to manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: The concentrations of IL-1β in tissue extracts of ovaries and livers were determined using an IL-1 beta Pro-form Mouse Uncoated ELISA kit (88-8014-22; Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Supernatants and cell pellets were collected from the interaction between IGRP-GFP CD8+ T-cells and βlox5 cells at a 10:1 ratio and were evaluated using a granzyme B ELISA kit (BMS2027; ThermoFisher Scientific, USA.). The standard curve was produced to determine the granzyme B concentration ...
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Immunology 2022Quote: ... Mouse lung homogenates were used to determine IgM protein using an IgM uncoated ELISA kit as directed (Invitrogen-ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... Mouse lung homogenates were used to determine IgM protein using an IgM uncoated ELISA kit as directed (Invitrogen-ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2020Quote: ... The cell supernatant was diluted 10-fold and the TNF-α content was measured with a mouse TNF-α Elisa kit obtained from Invitrogen. The remaining cells were subjected to CCK8 assay to detect cytotoxicity.
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bioRxiv - Bioengineering 2021Quote: ... The samples were allowed to culture for 12h at 37°C and cytokine concentration was measured in the media by Ready-Set-Go™ ELISA kits (Thermo Fisher) as detailed in the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Levels of murine Aβ (1–42) were measured by enzyme-linked immunosorbent assay (ELISA) using commercial kits (Thermo Fisher Scientific, cat# KMB3441) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Levels of phosphorylated proline-rich AKT substrate of 40kDa (phospho-PRAS40 (pT246)) were assessed using PRAS40 ELISA kits (Invitrogen, Camarillo, USA, KHO0421). Cells were lysed in extraction buffer (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of Aβ1-40 and Aβ1-42 in the s-extracts was determined by commercially available ELISA kits (ThermoFisher Scientific: KHB3481 and KHB3544). Aβ1-42 was undetectable in the Aβ negative sample ...
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bioRxiv - Immunology 2024Quote: Individual cytokines in supernatants from cell culture experiments were measured using the species-appropriate ELISA Kits mouse IL-6 (Invitrogen™ 88706488), mouse IL-1beta (Invitrogen™ 88701388) ...
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bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22, murine IL-6: Invitrogen 88-7064-88) and a SpectraMax M2 plate reader ...
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: RNA was isolated using the Qiagen RNeasy Mini Kit and reverse transcribed using the SuperScript III First-Strand Synthesis System (Invitrogen, #18080-51). qPCR was performed using SYBR Green PCR Master Mix (Lifetech ...
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bioRxiv - Genomics 2020Quote: ... cDNA from the extracted RNA was made using SuperScript™ III Reverse Transcriptase first strand kit (Cat. No: 18080093, ThermoFisher Scientific, USA) as per the procedures prescribed ...
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bioRxiv - Neuroscience 2020Quote: ... 400ng of total RNA from each brain was then used to synthesize 20μL of first-strand cDNA by reverse transcription (SuperScript III kit, Invitrogen, Camarillo, California, USA). 40ng of each cDNA preparation (a quantity calculated from the RNA concentration on the assumption that reverse transcription of RNA into cDNA was complete ...
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bioRxiv - Bioengineering 2020Quote: ... For qPCR one microgram of total RNA from each cell line in triplicate was reverse transcribed with Super Script III First Strand Synthesis Super Mix Kit (Invitrogen, Carlsbad, California). The relative abundance of Piezo1 cDNA fragments was determined using a SYBR-Green Master Mix (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was stored short-term at −20°C or long-term at −150°C or immediately used in a retrotranscription reaction (ProtoScript® AMV First Strand cDNA Synthesis Kit, New England Biolabs or SuperScript® III First-Strand Synthesis System, Invitrogen). In each reaction ...
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bioRxiv - Microbiology 2020Quote: ... Genomic RNA was then amplified in six equal-sized segments using overlapping primers sets (Supplementary Table S8) using SuperScript III One-Step RT-PCR kit (ThermoFisher Scientific, USA) with Platinum Taq ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA synthesis from each RNA samples was carried out by using cDNA synthesis kit SuperScript® III First-Strand Synthesis System for RT-PCR (Invitrogen, US). PCR reactions were set up in 10µl reaction mixture in 96-well PCR plates (Don et al. ...
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bioRxiv - Microbiology 2020Quote: ... TaqMan RT-qPCR was performed with the SuperScript™III Platinum™One-Step qRT-PCR kit (Invitrogen, Fischer Scientific, Illkirch, France) using the protocol described by Corman et al ...
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bioRxiv - Microbiology 2021Quote: ... The presence of SARS-CoV-2 RNA was determined by conventional reverse transcription PCR using the SuperScript®III One-Step RT-PCR System with Platinum® Taq DNA Polymerase kit (Invitrogen) in a 25μl reaction volume containing 12.5μl of 2X buffer ...
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bioRxiv - Immunology 2021Quote: Gene expression was assessed by RT-PCR as we described (25): cDNA was generated using SuperScript™ III First-Strand Synthesis System cDNA Synthesis Kit (Cat. No. 18080051) from Thermo Fisher, according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed from oligo(dT)20 primers (Integrated DNA Technologies) using the SuperScript III first strand synthesis kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 subgenomic RNA of the E gene was reverse transcribed and copy numbers quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the SuperScript III OneStep RT-PCR Kit (Invitrogen Life Technologies) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: One microgram of RNA was used for reverse transcription using Invitrogen’s SuperScript III First-Strand kit with the provided oligo-dT (ThermoFisher, cat # 18080-051). For qPCR ...
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bioRxiv - Physiology 2022Quote: ... and 1.5 μg of DNase treated RNA was used to synthesize cDNA using SuperScript III First-Strand Synthesis system for RT-PCR kit (Thermo Fisher Scientific) with Oligo (dT)20 primers according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from the gray matter of 5 CRL patients and 5 control patients (see Supplemental Table 1) using Trizol and cDNA was prepared using a Super Script III First Strand Synthesis kit (Invitrogen, Carlsbad, CA). Real time PCR was performed using the PrimePCR CSF3 assay qHsaCED0043218 from BIO-RAD ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using 5 µg RNA as a template per reaction using the Superscript III First Strand Synthesis System Kit (Invitrogen,18080-051) and random hexamer primers using the following protocol ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were amplified from extracted RNA using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA). A 20 μl reaction was assembled in PCR 8-tube strips through the addition of 10 μl 2× reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... First-strand cDNA was synthesized from 1 μg total RNA using the SuperScript III Reverse Transcriptase enzyme kit (Invitrogen, Carlsbad, CA, USA). Samples were labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies ...
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bioRxiv - Pathology 2020Quote: ... a one-step qRT-PCR was conducted in a final volume of 25 μL containing 5µL of extracted viral RNA from the SuperScript™ III Platinium™ One-Step qRT-PCR Kit (Life Technologies), 20 µL of a mix containing the probe/primer mix (Table 1) ...
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bioRxiv - Neuroscience 2021Quote: Single-stranded cDNAs were synthesized with the Superscript III first strand cDNA synthesis kit (Cat#: 18080051, Thermo Fisher Scientific, Waltham, MA, USA), according to the kit protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... between 0.2-1 µg of RNA was reverse transcribed using Superscript III First Strand Synthesis kit (Life Technologies cat. no. 18080-051) and oligo-dT primers to generate cDNA libraries.
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bioRxiv - Neuroscience 2021Quote: ... The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™). 5 μl of the resulting cDNA was used for a final volume of 20 μl PCR with indicated oligo primers ...
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bioRxiv - Immunology 2021Quote: ... a one-step RT-PCR was performed for 25 cycles using heavy chain BIOMED-2 variable antibody gene-specific primers as previously described 45–47 and the OneStep SuperScript III with Platinum® Taq High Fidelity kit (Invitrogen, 11304011).
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bioRxiv - Developmental Biology 2021Quote: ... the mixture is cooled on ice for 1 minute before adding reagents for reverse transcription from the SuperScript III Reverse Transcriptase Kit (Invitrogen Cat. 18080093) along with 1 unit of RNaseOUT Recombinant RNase Inhibitor (Invitrogen Cat ...