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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Transfection of 1 µg per well of plasmids encoding SARS-CoV-2 tag-free nucleocapsid protein was performed using 6 µL per well of Lipofectamine 2000 on HEK-293T cells with 70-80% confluency on 6-well plates (Invitrogen, 11668027). Compounds were added to the cells with fresh media after 6 hours of incubation with plasmids for 24-hour treatment before cell lysis and immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded at 30 000 cells/cm2 and were treated 6 h later with 6 µM of the calcium-sensitive Rhod2AM probe (ThermoFisher Scientific) for 1 h before refreshing the cell culture medium ...
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bioRxiv - Molecular Biology 2021Quote: The human HCT 116 colon cancer cell line was kindly provided to us by the Johns Hopkins Sidney Kimmel Comprehensive Cancer Center (Baltimore, USA) and cultivated in McCoy’s medium (Gibco™, Thermo Fisher Scientific Inc., cat n°22330-021). K-562 (ATCC ...
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bioRxiv - Microbiology 2023Quote: ... The template for generating homology-directed repair DNA for gene tagging was constructed by inserting a P2A viral skip peptide in frame with a downstream blasticidin-S deaminase (BSD) or puromycin N-acetyl-transferase (puro) and TOPO cloned into a pCR4 backbone (Thermo Fisher, Waltham, MA, United States of America) 51 ...
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bioRxiv - Plant Biology 2019Quote: To assess GUS expression driven by the maize ubiquitin promoter in transgenic barley transformed with the pBRACT214m-GUS construct we collected different tissues and stained these with 1mg/ml of X-gluc (5-bromo-4-chloro-3-indolyl-B-D-glucuronic acid, Thermo Scientific, USA) in X-gluc buffer (100mM sodium phosphate buffer pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: Overlay of EM and LM data (Fig. 3) and most of the segmentations (Fig. 4A,B,D,E) were done using Amira (Thermo Fisher Scientific). Additional segmentation was performed using Microscopy Image Browser (Belevich et al ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Microbiology 2022Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:3000; Thermo Fisher, HYB-076-08-02), rabbit anti- CFP-10 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:1,000; Thermo Fisher, HYB-076-08-02), rabbit anti-CFP-10 (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... at a 1:9 ratio (ng of DNA) using Lipofectamine 2000 prior to selection using 200 μg/ml of Hygromycin B (Thermo Fisher Scientific) for positive clones ...
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bioRxiv - Cell Biology 2019Quote: ... cultured neurons on 6-10 DIV were switched to pre-warmed Eagle’s minimum essential medium (EMEM, Thermo Fisher) supplemented with GlutaMax 1 hour before transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
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bioRxiv - Neuroscience 2019Quote: Neurons were grown on 6 cm dishes and treated with or without 10 μM chloroquine (Fisher Scientific, ICN19391980) in maintenance medium for 16 h ...
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bioRxiv - Physiology 2021Quote: ... 6-10 microliters of a mixture of green fluorescent particles (Thermo Scientific, 1.05 g/cm3, fluorescence 589 nm) were injected using a 2.5 μm Hamilton glass syringe with a pulled borosilicate capillary tube (Fig.2A) ...
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bioRxiv - Immunology 2020Quote: ... For poly(I:C) stimulation cells were transfected in 6-well plates with 10 μg polyI:C using Lipofectamine 2000 (Invitrogen) for 2 h before imaging ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ΔFZD1-10 or ΔLRP5/6 cells were transiently transfected in suspension using Lipofectamine 2000 (Thermo Fisher Scientific 11668). An approximately 80-90% confluent T-75 flask was re-suspended in 20 ml yielding a suspension with 4-5×105 of cells ml-1 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
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bioRxiv - Cell Biology 2024Quote: We seeded HEK293T cells at 2.5 × 10^6 cells per 15 cm plate in DMEM (Thermo Fisher 11965118), enriched with 10% FBS (Atlanta Biologicals S11150H) ...
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bioRxiv - Biochemistry 2024Quote: ... 6 second blot time and 10 second wait time before plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) set at 100% humidity and 4 °C ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... approximately 10 female and 6 male flies were placed into a flask (Fisher Scientific, Cat. No.:AS-355) of food and maintained at 25°C for one week to allow for mating and egg laying ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... buffer B:100 mM HFIP and 9 mM TEA in LC–MS grade methanol (Fisher Scientific); column ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted cell mixtures were resuspended in 10 μl of 1:9 collagen/setting buffer (10× Earle’s Balanced Salt Solution (Life Technologies), 0.2 M NaHCO3 and 50 mM NaOH) ...
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bioRxiv - Microbiology 2020Quote: ... sections of liver biopsies were performed as described previously (6) using a pan-genotype probeset (genotype A-D, VF6-20095, Affymetrix, CA. USA). Sections were stained with NBT (Nitroblue tetrazolium ...
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bioRxiv - Cell Biology 2020Quote: Glass-bottomed imaging dishes were exposed to UV light for 6 minutes and coated with Poly-D-Lysine (Thermo Fisher Scientific, A3890401) for a minimum of 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells (from B. D. Dynlacht) were grown in DMEM (Nacalai tesque) supplemented with 10% Calf Serum (Thermo Fisher Scientific) and 100 units/ml penicillin and 100 μg/ml streptomycin (P/S ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cell Biology 2019Quote: ... in addition to 7-Aminoactinomycin D (7AAD; ThermoFisher Scientific, Ref#A1310) for viability to monitor donor chimerism within the different lineages and the appearance of leukemic blasts characterized by a distinct scatter and lower GR1 and CD11b expression within the myeloid compartment ...
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bioRxiv - Immunology 2024Quote: ... with 20 μg/ml of 7-aminoactinomycin D (7AAD, Invitrogen A1310) in perm/wash (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 hours prior to collection organoids were incubated with 1 μM EdU (Thermo fisher, #C10636).
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Bioengineering 2020Quote: ... then incubated in 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindol) 1:10,000 (Thermo Fisher Scientific). Images were recorded using an Axioimager 2 Microscope with cooled CCD camera (SPOT Imaging Solutions ...
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bioRxiv - Neuroscience 2022Quote: ... using samples diluted to 1:1000 while interleukin (IL)-6 (Thermo Fisher Scientific, Cat. #BMS625) was evaluated using samples diluted 1:2 ...
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bioRxiv - Immunology 2022Quote: ... in 6-well plates and cultured in 1 ml/well RPMI-1640 medium (Thermo Fisher) containing 4% B-27 (Life Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... cell nuclei were stained with 1:1000 DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride, ThermoFisher) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific). Alexa Fluor 568 phalloidin was used to stain filamentous actin (F-actin ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Antibodies are listed in the Key Resources Table ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1:10K, Thermo Fisher Scientific) and mounted onto Superfrost slides (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and all sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 1:1000).
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bioRxiv - Immunology 2020Quote: ... the sections were stained with DAPI (4′, 6-diamidino-2-phenylindole; 1 μg/ml; Invitrogen). In the immunofluorescence study ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was done with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000; Thermo Fisher) for 5 minutes at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...