Labshake search
Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 5T4 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... CFTR expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
-
bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
-
bioRxiv - Cell Biology 2022Quote: ... Synthego, Redwood City, CA, USA), recombinant 2NLS-Cas9 nuclease (20 μM, Synthego, Redwood City, CA, USA) and optiMEM (Invitrogen, Waltham, MA, USA) supplemented with Y27632 (10 μM) ...
-
bioRxiv - Molecular Biology 2022Quote: ... was mixed with 2 µl of recombinant yeast Poly(A) Polymerase (PAP) together with 5 µl of 5X PAP buffer (Thermo Fisher Scientific), 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience) ...
-
bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
-
bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... and 33 µl of this buffer was added to the beads together with 10 µl Recombinant Proteinase K Solution (20 mg/ml, Thermo Fisher, AM2548) and 3 µl RiboLock RNase inhibitor (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... Fragments were end-repaired with Enzymatics low concentration end repair mix (Y9140-LC-L) and A-tailed with Recombinant Taq (Life Technologies, 100021276). Y-Adapters were pre-annealed by incubating a MiSeq Common Adapter with Adapters (A01-A10 ...
-
bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
-
bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
-
bioRxiv - Microbiology 2023Quote: ... The sequences of all four recombinant viruses generated in this study were confirmed by sequencing with a SeqStudio Genetic Analyzer (Thermo Fisher Scientific) and an outsourced service (Fasmac).
-
bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
-
bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
-
bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
-
bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
-
bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
-
bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
-
bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
-
bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
-
bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 constructs were tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
-
bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-β-PIX antibody (4515S; Cell Signalling Technology) and rabbit polyclonal anti-GST antibody (71-7500; Invitrogen).
-
bioRxiv - Neuroscience 2021Quote: ... 1:1,000] and secondary antibodies (horseradish peroxidase-conjugated anti-rabbit antibody [Cytiva, 1:2,500], Alexa Fluor 488-conjugated anti-rabbit antibody [Thermofisher, 1:2,500] ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with anti-rabbit Alexa 568 (1:1000; goat anti-rabbit IgG Alexa Fluor 568; Invitrogen #A11011) and anti-mouse Alexa A647 (1:1000 ...
-
bioRxiv - Microbiology 2022Quote: ... The membrane was further probed with 1:10,000 rabbit anti-PfExp2 primary antibody followed by 1:10,000 HRP conjugated secondary anti-rabbit antibody (Thermo Fisher) for 1h ...
-
bioRxiv - Immunology 2019Quote: ... conjugated rabbit anti-mouse (1:64) or AlexaFluor 594-conjugated rabbit anti-donkey antibody (1:250; Invitrogen, Paisley, UK). All secondary antibodies had been tested and found to be negative for cross-reactivity against human epithelial cells ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibodies were diluted as follows: rabbit anti-Cid51:2500 and goat anti-rabbit (Invitrogen Alexa Fluor 568, A-11011) 1:2000
-
bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies included monoclonal rabbit anti-ASO (Ionis, Carlsbad, CA) and polyclonal rabbit anti-AR (PA1-110, Invitrogen). The bound antibodies were detected using ImmPACT™ DAB Peroxidase (HRP ...
-
bioRxiv - Microbiology 2022Quote: ... incubated with secondary antibodies [Alexa Fluor 594- or 488 labelled goat anti-rabbit or donkey anti-rabbit (ThermoFisher Scientific)] diluted 1:1,000 in TBS supplemented with 0.5% BSA and 0.5 μg/ml of Hoechst 33342 for 45 min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-rabbit IgG -Alexa 488 or anti-rabbit IgG-Alexa 647 (A-11032, A-11034, A21245, Thermo Fisher Scientific). Nuclei were counterstained with 0.5 μg/mL DAPI (D9542 ...
-
bioRxiv - Immunology 2022Quote: ... The residual amount of endotoxin in the recombinant S100A4 preparation was examined using a Pierce™ Chromogenic Endotoxin Quant Kit (Thermo Fisher Scientific). Mice were anesthetized using isoflurane prior to immunization ...
-
bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
-
bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
-
bioRxiv - Plant Biology 2020Quote: The interaction of the NAB1 promoter fragments with recombinant LCRF-SBP (rLCRF-SBP) was analyzed by using the LightShift®Chemiluminescent EMSA Kit (Thermo Scientific™), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
-
bioRxiv - Biochemistry 2020Quote: Recombinant bacmids containing the gene of interest (PDI) were generated according to the Baculovirus Expression system manual (Thermo Fisher Scientific, Cat. No. 10359016). In brief ...
-
bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
-
bioRxiv - Microbiology 2022Quote: Cu-sensitive strains harboring the recombinant pTR plasmids were harvested for metal content determination by using inductively coupled plasma mass spectrometry (ICP-MS) (Thermo Fisher Scientific, USA). E ...
-
bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2020Quote: ... The linearized recombinant plasmid was used as templates to synthesize capped RNAs (cRNAs) with the mMessage mMachine T7 Kit (Life Technologies, Carlsbad, CA). The cRNAs were dissolved in RNase-free water at a concentration of 1000 ng/μL ...
-
bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...