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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fura-2-acetoxymethyl ester (Fura-2-AM) and Pluronic F-127 were purchased from Molecular Probes (Eugene, OR, USA) and dissolved in dimethylsulfoxide (DMSO) ...
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bioRxiv - Biochemistry 2020Quote: In vivo Δψm was measured using the cell-permeant red-fluorescent dye TMRE (tetramethylrhodamine ethyl ester, Thermo Fisher Scientific). For each time point ...
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bioRxiv - Bioengineering 2020Quote: ... the cell mixtures were stained with 2.5 µM carboxyfluorescein diacetate succinimidyl ester (CFDA SE, ThermoFisher, referred to as CFSE) before restimulation with anti-CD3/CD28 beads ...
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bioRxiv - Biochemistry 2020Quote: ... NosRcaΔC and NosRca-IFmut was labelled at the N terminus with the fluorophore Alexa Fluor 405 NHS ester (ThermoFisher) (∼2.2 ...
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bioRxiv - Biophysics 2021Quote: ... as specified by the commercial protocol) and a fluorescent tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Biophysics 2020Quote: A1-LCD was fluorescently labeled on the N-terminus using Alexa Fluor® 488 NHS ester fluorescent dye (ThermoFisher) to label the primary amines of proteins ...
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bioRxiv - Immunology 2022Quote: ... The barcoding dyes which were used in this study were Pacific Orange and Alexa Fluor 700 NHS esters (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Purified vesicles were covalently labelled with an amine-reactive Alexa Fluor 488 5-SDP ester dye (Molecular Probes/ThermoScientific).
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: Fn purified from human plasma (sigma) or monoclonal antibodies were fluorescently conjugated using AlexaFluor 488 and 647 Ester (Invitrogen). The conjugation and purification were done according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... animals previously injected with vaccine or PBS where injected 24 hrs before sacrifice in the same footpad with 30 μL of 0.5 mM 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen). For assessing migration after 24 hrs ...
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CRISPR-Csx28 forms a Cas13b-activated membrane pore required for robust CRISPR-Cas adaptive immunitybioRxiv - Biochemistry 2021Quote: Protein in vivo crosslinking was carried out with NHS ester, DSS (disuccinimidyl suberate, spacer arm 11.4 Å) (Thermo Scientific) also as suggested by the manufacturer ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... non-viable Nb were incubated in 0.05 M NaHCO3 buffer and 0.1 mg AF488 NHS Ester (Molecular Probes, Invitrogen) for 15 minutes and washed with 0.1M Tris buffer according to the manufacturer’s instructions.
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... non-viable Nb were incubated in 0.05 M NaHCO3 buffer and 0.1 mg AF488 NHS Ester (Molecular Probes, Invitrogen) for 15 minutes and washed with 0.1M Tris buffer according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... Bovine fibrinogen is labeled with a fluorescent reactive dye (Alexa Fluor 647 NHS succinimidyl ester, Thermo Fisher Scientific, Waltham). After purification from the unreacted dye via dialysis ...
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bioRxiv - Neuroscience 2022Quote: ... by incubation for 40 min in Krebs solution containing 20 μM calcein AM (acetoxymethyl ester of calcein; ThermoFisher Scientific). The slices were then washed three times in normal Krebs solution and fixed for at least 2 hours at RT or overnight at 4 °C in PBS PAF 4% ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysate from a T75 flask was labelled with 50 μg Alexa Fluor 647 succinimidyl ester (ThermoFisher Ref A20006) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CM for 48 hours before loading with 2 μM fura-2 acetoxymethyl ester (Molecular Probes) in calcium imaging buffer (CIB ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were stained with carboxifluorescein diacetate succinimidyl ester (CFSE) using the CellTrace CFSE Cell Proliferation Kit (Invitrogen, C34554) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester) acquired from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biophysics 2023Quote: BRICHOS was labeled with Alexa-488 by mixing 43.6 µM BRICHOS dissolved in 100 mM bicarbonate buffer (pH 8.3) and Alexa-488 NHS Ester (Thermo Scientific) dissolved in DMSO in a 1:2 molar ratio followed by 1 h incubation at RT ...
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bioRxiv - Microbiology 2022Quote: ... Virions were then labeled with 100 µg of Alexa Fluor™ 488 TFP ester following the manufacturer’s instructions (ThermoFisher). Unincorporated dyes were separated from labeled virions using PD-10 gel filtration columns ...
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bioRxiv - Cell Biology 2023Quote: ... We washed the beads twice with phosphate-buffered saline (PBS) and incubated them with 0.5 mL PBS containing 0.05 µg Rhodamine Red-X succinimidyl ester (ThermoFisher Scientific) for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... and α-synuclein) were mixed with an excess of N-hydroxysuccinimide (NHS)-ester-functionalized Alexa 488 dye (Thermo Fisher). The dye-to-protein ratios were optimized to maintain an average degree of labeling (DOL ...
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bioRxiv - Immunology 2024Quote: Immune cells were isolated as described above then stained with the following antibodies (Live Dead AF350 NHS Ester, Thermofisher, #A10168 ...
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bioRxiv - Immunology 2024Quote: ... HT29 cells were washed twice with PBS and incubated with 5 μM pHrodo Red-succinimidyl ester (Invitrogen, Cat# P36600) with rotation at r.t ...
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bioRxiv - Immunology 2024Quote: Cytiva Ficoll-PaqueTM Plus Medium was from Fischer Scientific (Gothenburg, Sweden), Dextran T500 was from Pharmacosmos (Holbæk, Denmark) whereas Fura-2-acetoxymethyl ester (AM) was from Invitrogen by Thermo Fischer Scientific (Gothenburg ...
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Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial functionbioRxiv - Cell Biology 2024Quote: ... HepG2 cells were harvested with 0.05% Trypsin-EDTA and resuspended in PBS with 50 nM tetramethylrhodamine ethyl ester (TMRE, T669, Invitrogen) for 25 minutes at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split every 3 days at 1:10 using Trypsin-EDTA (ThermoFisher Scientific). On day 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1/10th volumes of 3 M Sodium Acetate and 30 μg Glycoblue coprecipitant (Ambion).
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bioRxiv - Developmental Biology 2022Quote: Testes were dissected from 1-3 day old males in SF-900 media (Gibco) then fixed in 4% formaldehyde diluted in PBS for 20-30 minutes and permeabilized in 1% PBT (triton x100 diluted in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The epithelium medium was composed of a 3:1 mixture of DMEM (Hyclone, ThermoFisher) combined with Ham’s F12 medium (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Axotomy was performed using 3 different methods: 1) Trypsin-EDTA 0.25% (Thermo Fisher Scientific) treatment for 15 min (Figure 4C ...
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bioRxiv - Cell Biology 2021Quote: ... 1-3 µg were reverse transcribed with a MuLV reverse transcriptase (Applied Biosystems, UK) using random primers (Bioline ...
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bioRxiv - Molecular Biology 2022Quote: ... using NuPAGE 3-8% Tris-Acetate 1 mm 10-well gels (Thermo Fisher Scientific) run at 150 V for 90 minutes in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Organoids were washed and fresh medium containing TOPRO-3 Iodide (1/1000, Molecular Probes) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... sonicated (3 × 0.5s pulses) with a probe sonicator (Level 1; Fisher Scientific, Waltham, MA) and quantified using a Pierce™ 660 nm assay (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... the chambers were washed 3 times using 1× Hank’s balanced Salt Solution (HBSS, Gibco) and blocked with 1% Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... this included TO-PRO®-3 Iodide nuclear dye (T3605, Life Technologies 1:10,000). Slides were washed 3x PBST prior to mounting with anti-fading PVA/Moviol-DABCO (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated in 1 μM TO-PRO-3 (T3605; Thermo Fisher Scientific, Waltham, MA) in PBS for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated with rabbit anti-Neuroligin-3 (1:250, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-claudin 3 polyclonal antibody (1:100 dilution, PA5-16867, Thermo Fisher Scientific); mouse anti-chondroitin sulfate antibody (1:100 dilution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL NT-3 (PreproTech) and 1 µg/mL Laminin (Gibco, Thermo Fisher) plus DOX ...