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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Biochemistry 2022Quote: ... A high-titer virus stock for hPolεCD(insect) was obtained by using the Bac-to-Bac Baculovirus Expression System from Invitrogen. 1.8×109 Sf21 cells in 1 L shaking culture were infected with the recombinant virus at a multiplicity of infection of 2 and cultivated at 27 ºC for 56 hr ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Microbiology 2020Quote: ... Two microliters of extracted RNA were amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Microbiology 2020Quote: ... one for virus quantitation (TCID50) and the other for vRNA extraction in TRIzol (ThermoFisher, 15596026; 1 in 3 dilution). Samples were stored at -80°C until required ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed according to the manufacturer’s instructions using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2022Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in complete media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Foetal Bovine Serum (FBS ...
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bioRxiv - Pathology 2022Quote: ... RNA was reverse transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was amplified by RT-qPCR using the Taqman Fast Virus 1-step master mix (Thermo Fisher Scientific). Primers and probes targeting SARS-CoV-2 RNA-dependent RNA polymerase were described in [25] ...
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bioRxiv - Genomics 2019Quote: ... we reprogrammed fibroblast samples from the 181 individuals in this study using non-integrative Cytotune Sendai virus (Life Technologies) (Ban et al. ...
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bioRxiv - Cell Biology 2019Quote: ... complementary DNA was then reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Invitrogen), using random hexamer oligonucleotides for priming ...
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bioRxiv - Microbiology 2020Quote: ... The purified virus was dissolved with an equal volume of 2x Tris-Glycine SDS Sample Buffer (Thermo Fisher Scientific) and boiled for 5 min without a reducing agent ...
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bioRxiv - Cancer Biology 2019Quote: ... Expression levels of MYC and MEIS1 transcript were quantified using TaqMan Fast Virus 1-Step Master Mix (Life Technologies) using PrimeTime qPCR Probe Assays from Integrated DNA Technologies in a 3:1 ratio of primer to 5′ 6-FAM and 3′ TAMRA labeled probe ...
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bioRxiv - Microbiology 2020Quote: ... and infected at an MOI of 1 with the VSVΔG:mNeon/VSV-G virus diluted in 10 mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) mock- or virus-infected (MOI of 0.01) were extracted using TRIzol reagent (Thermo Fisher Scientific). Superscript® II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Virus was generated by transfecting 3×106 HEK293T cells with plasmids using Lipofectamine 2000 (Thermo Scientific, Cat. No. 11668019) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... and then inoculated intranasally (i.n.) with 30 μL of virus (0.5 HAU) diluted in Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, Loughborough UK). Mock-treated control mice were inoculated similarly with DPBS ...
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bioRxiv - Microbiology 2021Quote: ... was propagated on Vero E6 cells at a multiplicity of infection (MOI) of 0.001 in virus diluent (DMEM supplemented with 2% FBS, 1X Penicillin/Streptomycin (Gibco), 1mM sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Vero FM cells were infected with passage 3 stock at an MOI of 0.01 in virus infection medium [DMEM (Gibco) containing 2% FCS (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2020Quote: ... and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies) targeting the gene E ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were reprogrammed into iPSCs by using the episomal Sendai virus approach (CytoTune-iPS 1.0 Sendai Kit, Life Technologies) to obtain hiPSCs ...
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bioRxiv - Immunology 2022Quote: ... according to manufacturer’s recommendation and qRT-PCR was performed using TaqMan Fast Virus 1-Step Mix (Applied Biosystems, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Viral stocks were passaged and expanded using MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/mL bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2022Quote: ... and then quantified by qRT-PCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with the TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
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bioRxiv - Microbiology 2023Quote: The RNA of H1N1pdm09 was extracted from virus samples using a PureLink™ viral RNA/DNA mini kit (Invitrogen). For qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin used for virus propagation was standard cell culture grade 0.25% porcine trypsin with EDTA and phenol red (ThermoFisher). Huh-7 cells were seeded on a 24-well plate and incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral plaques were passaged at an MOI of ∼0.001 in MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/ml bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were prepared by three passages in Vero (ATCC CCL-81) in Dulbecco’s modified Eagle Medium (DMEM) (Gibco) with 1% Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
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bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...
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bioRxiv - Microbiology 2023Quote: ... The RT-PCR was performed using the Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a QuantStudio3 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells infected with pLENTI-Luciferase-expressing virus (generous gift of Charlotte Kuperwasser, Tufts University) were selected with neomycin (G418, 500μg/mL, Gibco) for 2-3 weeks ...
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bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...