Labshake search
Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... Cells were then biotinylated using NHS-PEG4-Biotin (125 µM unless otherwise specified, ThermoFisher Cat. No. A39259) diluted in PBSCMA to achieve a total volume of 50 µL per well for 30 minutes at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... Up to 150 ng of the DNA was then biotinylated with Streptavidin C-1 beads (ThermoFisher Scientific). Tagmentation was performed using Nextera DNA Library Preparation Kit (#FC-121-1030 ...
-
bioRxiv - Neuroscience 2020Quote: ... was added to each sample and mRNA was captured using modified oligo-dT biotinylated beads (Dynabeads, Invitrogen). PCR amplification was performed for 22 cycles ...
-
bioRxiv - Cancer Biology 2021Quote: ... 50 pmol Biotinylated RNA oligos were conjugated with 50 μl of streptavidin beads (50% slurry; Thermo Fisher) in a total volume of 300 μl of RNA-binding buffer (20mM Tris ...
-
bioRxiv - Bioengineering 2020Quote: ... Antibodies were biotinylated using an EZ-Link™ micro Sulfo-NHS-biotinylation kit (ThermoFisher Scientific, Waltham, MA). For surface marker EV detection ...
-
bioRxiv - Bioengineering 2021Quote: ... The absorbance spectrum of UV-cleavable biotinylated cDNA-antibody conjugates were measured by a Nanodrop spectrophotometer (Thermofisher) to determine the degree of labeling (DOL ...
-
bioRxiv - Biophysics 2021Quote: ... the respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was resolved in H2O and directly added to the protein solution in a final molar ratio of 1:1 in 25 mM MES ...
-
bioRxiv - Microbiology 2021Quote: ... the RBD was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA).
-
bioRxiv - Immunology 2021Quote: ... biotinylated SARS-2 and Rs4081 RBDs were incubated with streptavidin-APC (eBioscience™) and streptavidin-PE (ThermoFisher), respectively ...
-
bioRxiv - Cell Biology 2021Quote: To prepare biotinylated salmon sperm DNA for DNA-carpets 10 mg/ml salmon sperm DNA (Thermo Fisher) was sonicated for 5 min (110 watts ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 790 ng of the products were biotinylated with Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher Scientific). After ligation of 15 h ...
-
bioRxiv - Microbiology 2020Quote: ... RBD-6H (340-538; NITN.GPKK) was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256; Life Technologies). Serial half-log dilutions (starting at 1 μM ...
-
bioRxiv - Genomics 2019Quote: ... and the 5’ overhang was repaired using a biotinylated residue (0.4 mM biotin-14-Datp (INVITROGEN, America). The resulting blunt-end fragments were ligated in situ (10X NEB T4 DNA ligase buffer (NEB ...
-
bioRxiv - Molecular Biology 2019Quote: EMSA was performed using double stranded biotinylated DNA probes and the Lightshift Chemiluminescent EMSA kit (Thermo Scientific). Biotin 3’ end-labeled DNA oligomers were prepared using a biotin end-labeling kit (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2019Quote: ... or PD41 underwent stereotaxic surgery to inject biotinylated dextran amine (BDA; 10,000MW anterograde neural tracer; Life Technologies NeuroTrace BDA 10000 Neuronal Tracer Kit ...
-
bioRxiv - Genomics 2019Quote: ... Biotinylated primers and undigested inserts were removed as before using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific). Ligation of pJDrcEPP_lib and minP-Luc2 inserts was performed at a 1:3 ratio as before but with T7 DNA ligase (New England Biolabs) ...
-
bioRxiv - Microbiology 2019Quote: ... Anti-NS1 antibody binding was detected by a biotinylated goat anti-mouse IgG secondary antibody solution (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... The following secondary antibodies were used: biotinylated donkey anti-goat IgG (H+L) (Molecular Probes, Paisley, UK) and biotinylated donkey anti-rat IgG (H+L ...
-
bioRxiv - Biophysics 2020Quote: ... The respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly resolved in H2O ...
-
bioRxiv - Neuroscience 2019Quote: ... we injected the axon tracer biotinylated dextran amine (BDA, 10,000 MW, 10% solution in water, Invitrogen, D1956) into the sensorimotor cortex 2 weeks before the mice were sacrificed ...
-
bioRxiv - Neuroscience 2019Quote: ... A hybridization cocktail containing the biotinylated target was incubated with the GeneChip Mouse 2.0 ST array (Affymetrix) at 60rpm for 16hr at 45°C in a Genechip Hybridisation Oven 645 ...
-
bioRxiv - Microbiology 2020Quote: ... the RBD was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA). The assay was carried out by placing the Streptavidin Biosensors (ForteBio ...
-
bioRxiv - Immunology 2021Quote: ... fragments containing a biotinylated C region were captured by M270 beads which were crosslinked to streptavidin (Invitrogen), with a dissociation ...
-
bioRxiv - Immunology 2019Quote: ... Clone 31-90-25 was biotinylated with the EZ-Link Micro Sulfo-NHS-Biotinylation Kit (Pierce, ThermoFisher). Samples were detected using streptavidin-HRP (Fitzgerald ...
-
bioRxiv - Neuroscience 2019Quote: ... biotinylated dextran amine (BDA, 10 kDa; 10% solution dissolved in PB; Thermo Fisher Scientific, Waltham, MA, USA) was injected using either a brief air pulse (10–20 psi for 40–100 ms ...
-
bioRxiv - Biochemistry 2021Quote: ... biotinylated Ube2k-UBC∼Ub was immobilised to Nunc Maxisorp 96-well plates (Fisher Scientific, Nepean, ON, Canada) coated alternately with neutravidin (New England BioLabs ...
-
bioRxiv - Molecular Biology 2020Quote: EVs were biotinylated using EZ-Link Sulfo-NHS-LC LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA). Biotinylated EVs were loaded onto Zeba spin desalting columns (7 K MWCO ...
-
bioRxiv - Molecular Biology 2021Quote: ... Where required antibodies were biotinylated using EZ-link NHS-LC-Biotin according to the manufacturer’s instructions (Thermofisher), or were attached to cyanogen bromide-activated agarose using the manufacturer’s protocol (GE Healthcare).
-
bioRxiv - Immunology 2022Quote: ... The trimeric spike SARS-CoV-2 and its variants were biotinylated (EZ-Link Sulfo-NHS-Biotin, ThermoFisher), desalted (Zeba Spike Desalting ...
-
bioRxiv - Immunology 2022Quote: ... Bovine serum albumin (BSA) was biotinylated using the EZ-Link Micro NHS-PEG4-Biotinylation Kit (Thermo Fisher); excess unreacted biotin was removed using 7-kDa Zeba desalting columns (Pierce).
-
bioRxiv - Microbiology 2022Quote: ... Biotinylated lectins were precomplexed (on ice, 20 min) with 0.2 µg streptavidin Alexa Fluor 488 (S32354, Invitrogen). For titration experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... Then 0.1 μl of 10% biotinylated dextran amine (BDA, 10,000 MW, ThermoFisher Scientific, Waltham, MA, United States) or 4% FluroGold (FG ...
-
bioRxiv - Neuroscience 2022Quote: ... Biocytin and biotinylated WFA were both visualized using streptavidin conjugated to Alexa Fluor 647 (Invitrogen S21374, RRID:AB_2336066). These antibodies have previously been shown to have high specificity (Hitti & Siegelbaum ...
-
bioRxiv - Microbiology 2022Quote: SEC purified PfRH5 in 1X PBS was chemically biotinylated using EZ-LinkTM Sulfo-NHS-Biotin (Thermofisher Scientific) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 pmol of biotinylated DNA were coupled to 10 μL Dynabeads MyOne Streptavidin T1 (Thermo Fisher Scientific) and free ends blocked with 3 pmol streptavidin afterwards ...
-
bioRxiv - Biochemistry 2024Quote: ... TcA(914Cys) and TcA(1279Cys) were biotinylated using EZ-link NHS-PEG12-Biotin (ThermoFisher, cat. no. A35389). Biotinylation was performed in labelling buffer simultaneous to the labelling reaction with BFL and Atto647N ...
-
bioRxiv - Molecular Biology 2024Quote: ... Biotinylated bridge adapter and RNA-DNA chimeras were then pulled down using streptavidin beads (Thermo Fisher, 65002) and a switch template oligonucleotide (IDT ...
-
bioRxiv - Genomics 2024Quote: ... DNA molecules containing biotinylated ligation junctions were pulled down on Dynabeads MyOne Streptavidin T1 beads (Life Technologies). For each capture ...
-
bioRxiv - Immunology 2024Quote: ... cloned and expressed in-house) were biotinylated using EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific) with 50-fold molar excess biotin for 48hr at 4 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... The detecting antibody was the biotinylated HT7 that recognizes residue 159-163 of tau (Thermo Fisher Scientific). To measure p-Tau396 ...
-
bioRxiv - Cell Biology 2023Quote: ... biotinylated molecules were pulled down using the Pierce™ Cell Surface Biotinylation and Isolation Kit (Thermo Fisher). Proteins were not removed from the beads as per the kit’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Biotinylated DNA fragments were then purified using activated beads from the Dynabeads® kilobaseBINDER™ kit (Invitrogen). Following this ...
-
bioRxiv - Developmental Biology 2023Quote: ... Biotinylated nascent RNA was captured according to protocols within the Click-iT Nascent RNA Capture Kit (Invitrogen) and used in RT-qPCR.
-
bioRxiv - Molecular Biology 2023Quote: ... Biotinylated peptides (Biotin-PNTAYQK(Ac)QQDTLI) previously immobilized on magnetic Streptavidin beads (Dynabeads MyOne Invitrogen™ 65601) were incubated in presence or absence of SIRT1 −/+ NAD+ (0.5 nM ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1 μl 10 μM 5‘-biotinylated oligo-dT30VN (IDT) and 1 μl 10 mM dNTP (Thermo Scientific). Cells were sorted at one cell per well ...
-
bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 RBD (BEI Resources NR-52309) was biotinylated with Sulfo-NHS-LC Biotin (Thermo Fisher), then incubated with 1μm fluorescent neutravidin beads (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... a solution of biotinylated primary antibodies in DPBS + 3% fetal bovine serum + 10mM EDTA (Invitrogen, 15575-038) was prepared ...
-
bioRxiv - Immunology 2024Quote: ... Biotinylated RBD was subjected to Zeba™ Spin Desalting Columns (7k MWCO) (Thermo Scientific, USA, catalogue 89889) to remove excess biotin.
-
bioRxiv - Immunology 2024Quote: ... The bat albumin was biotinylated using EZ-Link® NHS-SS-Biotin (Thermo Fisher Scientific, Ref#21441) following a protocol recommended by the manufacturer ...
-
bioRxiv - Biochemistry 2024Quote: ... biotinylated monomeric EVH1 domain (1 µM) was pre-tetramerized by incubation with streptavidin-phycoerythrin (SA-PE; ThermoFisher) for 30 min ...