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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... #13354) was immobilized at 1 µg/mL on 96-well assay plates (Nunc Maxisorp, Thermo Fisher Scientific, Waltham, MA). Plates were then blocked with 5% w/v BSA and incubated with clarified cell lysates containing 200 ng/mL total protein followed by incubation with a rabbit polyclonal anti-GRFT antibody (gift of Barry O’Keefe ...
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bioRxiv - Molecular Biology 2022Quote: ... ΔagrA and strains obtained the IVEE at 30°C and transformed with pGID310 were grown overnight in BHI supplemented with Chl at 30°C and streaked in BHI agar plates supplemented with X-Gal 0.1 mg.mL-1 (Thermo Scientific) and incubated for at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 15 min at room temperature with 1-Step Turbo TMB-ELISA Substrate Solution (Thermo Fisher), then terminated with equal volume of 1M phosphoric acid ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated at 140’000 cells / well in a 24-well plate in 1 ml DMEM-GlutaMax (ThermoFisher #10566016) containing 10 % fetal bovine serum for 18 h prior to plasmid transfection ...
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bioRxiv - Microbiology 2020Quote: ... ZIKV-NS1 and MV-H were similarly resuspended in coating buffer (50 mM Na2CO3 [pH 9.6]) at a concentration of 1 μg/ml and then plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl per well ...
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bioRxiv - Microbiology 2020Quote: ... was resuspended in a coating buffer (50 mM Na2CO3 [pH 9.6]) at a concentration of 1 μg /ml and then plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl in each well ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated (250,000 cells per well in a 24 well plate) in plating media (DMEM, 1% penicillin-streptomycin, Glutamax (Gibco), 10% horse serum ...
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bioRxiv - Microbiology 2021Quote: ... The remaining co-cultures were diluted 1:10 to OD600nm 0.01 and pipetted into a round bottom 96-well plate (Nunc). Growth measurements were performed as described above for 72 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Six overnight cultures were diluted 1:1,000 into fresh LB in flat-bottom 96-well plates (Thermo Scientific, Denmark), which were incubated during 24 hours at 37 ºC 250 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Embedded organoids were transferred to a 6-well low attachment plate and maintained in organoid differentiation medium (DMEM:F12 containing 1× N2 and B27 supplements (with no vitamin A, Invitrogen), 1× penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2019Quote: ... and 100g/ml streptomycin-sulfate at a concentration of 1×106 cells/ml in 24-well plates (Thermo Scientific) that were either uncoated (unstimulated cells ...
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bioRxiv - Cell Biology 2019Quote: ... H4 cells were seeded into a 96-well plate at a density of 1 cell per well in 200 μL Iscove’s Modified Dulbecco’s Medium (Thermo Fisher). Clones were grown to confluency then expanded and screened for successful knockout deletion by Western blotting.
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bioRxiv - Synthetic Biology 2020Quote: ... Starter cultures were then diluted 1:100 into M9sa in Nunc™ Edge 2.0 96-well plates (Thermo Fisher). Cells were grown in the presence of the indicated amount of aTc in a Spark plate reader (Tecan ...
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bioRxiv - Biochemistry 2021Quote: ... and complemented with 7.5 μM of Site2hp RNA (4:1 protein:RNA molar ratio) in PBS buffer in a 384 black-well clear-bottom plate (Nunc). A ThT stock was prepared as described above and added to the well to 300 μM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... EBs were transferred to ultra-low attachment 24 well plates and neuroinduction medium was added [1% N2 supplement (Gibco), 1% GlutaMAX (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were seeded at low and high density in 6-or 12-well plates coated with 0,2% type 1 Collagen (Gibco) and grown in PM for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... Plates were blocked for 1 hr at room temperature with 200 μl 5% Blocker™ BLOTTO (Thermo Scientific, #37530) and washed three times with 200 μl PBS-T ...
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bioRxiv - Genomics 2021Quote: ... and plates were washed once with PBS and then fixed for 10 minutes with 1% formaldehyde (Thermo Fisher Scientific) in PBS at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plates were washed three times and then developed using 50 μL 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher). A similar ELISA protocol was followed for detecting RNC complexes displaying glycosylated DQNATpd except that the plates were coated with 50 μL of 5-μg/mL mouse mAb FB11 in PBS and were subsequently detected with HRP-conjugated anti-mouse IgG.
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bioRxiv - Plant Biology 2022Quote: ... The luminescence was detected for 40 min with 1 min interval using Varioskan Flash plate reader (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed four times and incubated with 3,3’,5,5’-tetramethylbenzidine (TMB, 1-Step Ultra TMB solution, ThermoFisher, #34029) for 20-30 min in the dark ...
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bioRxiv - Microbiology 2022Quote: C2C12 myoblasts (1 × 105 cells/well) were cultured in six-well culture plates in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen-Gibco), containing 10% fetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... and washing the plate with an additional 1 ml of 1X PBS supplemented with antibiotic/antimycotic solution (Gibco, USA). Nasal washes were then aliquoted and frozen at −80°C until further processing ...
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bioRxiv - Biochemistry 2024Quote: ... Prepared plates were centrifuged at 2250 rcf for 1 min and sealed using MicroAmp Optical Adhesive Film (ThermoFisher Scientific). Melt experiments were performed using a QuantStudio Real-Time PCR thermocycler (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The absorbent (A450) was measured by the plate reader after HRPO substrate (1-Step Ultra TMB-ELISA, ThermoFisher Scientific) was ...
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bioRxiv - Microbiology 2023Quote: ... The plates were slapped dry and blocked for 1 hour at 37°C using Casein in PBS (Thermo Fisher). Following blocking ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Microbiology 2023Quote: Co-cultures protist:bacteria were established in 0.01 M Tris-HCl pH 7.5 buffer as described above (1:200 protist:bacteria ratio) and transferred to a 96 well black plate (Thermo Scientific). GFP fluorescence was monitored automatically by measuring green fluorescence with 480 nm excitation and 512-552 nm emission wavelengths nm with a TECAN Infinite MPlex plate reader (Tecan Group Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... the plate was developed using 50 µl of 1-Step TMB Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-NKp46 and NKp30 agonist antibodies (R&D) were coated (1 µg/mL) in NUNC 96-well plates (Thermofisher) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... AB+ heat inactivated serum with 0.5%(v/v) gentamycin per condition in 1 mL volumes in 24-well plates (Nunc). The plates were incubated at 37°C and with mixed gas (5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Neuroscience 2023Quote: PFB-FDGlu GCase activity assay: Cells in 96-well plates were pre-loaded for 30 minutes with lysotracker deep red (1:20,000, Invitrogen). Media was then exchanged for FluoroBrite imaging media (Thermo ...
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bioRxiv - Neuroscience 2024Quote: ... confluent iPSC cultures were detached using 0.5mM EDTA and plated 1:4 on Matrigel ES-coated plates in StemFlex Medium (Gibco) supplemented with 10μM ROCK inhibitor (SellChem) ...
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bioRxiv - Microbiology 2024Quote: ... cells were plated at 1×106 in 4 10cm plates 24 hours prior to harvest through trypsinization (ThermoFisher Scientific) for adherent cells ...
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bioRxiv - Developmental Biology 2024Quote: Confluent wells of iPSCs cultured on Geltrex-coated plates in mTeSR-1 medium were singularized with Accutase (ThermoFisher Scientific) and plated on Geltrex coated plates at 4.5-6 ×104 cells/cm2 in DMEM/F-12 ...
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bioRxiv - Neuroscience 2024Quote: ... 100’000 for 24WP) onto the PLL/Laminin coated plates with neuronal differentiation medium (B27 supplement 1:50 (17504001, Gibco), Glutamax 1 mM ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated IgG retained on plates was reacted with a 1:10,000 dilution Streptavidin-HRP conjugate (Thermo Scientific, cat. N504) in ADA-WB containing 10 mg/ml BSA for one hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the cells were seeded at a density of 7 x 105 cells/well and cultured overnight in a 24-well plate (TPP) with 1 ml of Dulbecco’s Modified Eagle medium (DMEM, Gibco) supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2024Quote: ... the plate was washed six times and developed with 1-Step™ TMB ELISA Substrate Solutions (Thermo Fisher Scientific). The absorbance was measured at 450 nm using a SpectraMax M2 microplate reader (Molecular Devices) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 96-well plate (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc) were coated with the appropriate antigen (S1 protein 1.2 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 96-well ELISA plates (Nunc, Germany) were coated with SARS-CoV-2 specific antigen (S1-RBD at a concentration of 1.5μg/well in PBS pH 7.4) ...
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bioRxiv - Neuroscience 2021Quote: ... Custom TaqMan array plates (Thermofisher, 4413261) were used for the Wnt ligand screen ...
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bioRxiv - Cell Biology 2022Quote: ... black optical 96 well plates (Nunc) and allowed to attach overnight ...
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bioRxiv - Immunology 2022Quote: MaxiSorp ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml purified RBD or S protein in 1xBBS (140 mM NaCl ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... MaxiSorp ELISA plates (Thermo Fisher Scientific) were coated with 10-20µg/ml of anti-mouse immunoglobulin (Ig ...
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bioRxiv - Microbiology 2022Quote: ... High-binding ELISA plates (Thermo Scientific) were coated with capture antibody and incubated overnight at room temperature ...
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bioRxiv - Immunology 2019Quote: ... flat MaxiSorp 96-well plates (Nunc) were coated overnight with the vaccine (diluted 1:3000 in DPBS ...