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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Human TS cells were cultured in six-well plates precoated with 5 μg/mL of CorningTM collagen IV (CB40233, Thermo Fisher) and Basal Human TS Cell Medium [Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12 ...
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bioRxiv - Immunology 2024Quote: ... with 312,500 B cells for 1 h prior to adding to 24 well plates coated with 5 µg/ml anti-CD3 with or without a permeable polycarbonate cell culture insert (Fisher Scientific) in the presence of 2 µg/mL soluble anti-CD28 ...
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bioRxiv - Biochemistry 2024Quote: ... Biolog Microbial Phenotyping Microarray Plates PM1-PM6 were resuspended in 125 μL MilliQ water and 5 μL of each well was aliquoted into MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific) to prepare screening plates ...
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bioRxiv - Biochemistry 2024Quote: ... The well-plate was subsequently incubated at 30 °C for 50 minutes in the thermocycler QuantStudio 5 real-time PCR system (Applied Biosystems). SYBR® Green I fluorescence was recorded each minute throughout the incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amoebae were grown from frozen spores at room temperature on 100 x 15mm plates filled with 30mL SM/5 agar (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Pathology 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.5 × 105 cells were seeded on a fibronectin coated well of a 12 well plate in GK15 medium (GMEM [Life Technologies] with 15% KSR ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µL of template DNA (5-10 ng) were run on 96-well plates on a StepOne Plus Real-Time PCR system (ThermoFisher Scientific). A melting curve analysis was carried out to confirm the amplification of a single product in each reaction ...
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bioRxiv - Microbiology 2023Quote: ... Colony forming units (CFUs) of frozen stocks were calculated based on serial dilution spotting on TSA + 5% sheep’s blood plates (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... At 5-6 dpf each FoxP2.A:FingR(PSD95)+ larva was placed into individual wells of a 6-well plate (Thermo Fisher Scientific) containing approximately 10mL of fish water ...
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bioRxiv - Molecular Biology 2023Quote: ... Raji cells were seeded in 96-well plate (5 x 104/well) and co-cultured with T cells stained with CellTraceTM Violet (Invitrogen, USA) at the indicated E:T ratios ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 KELLY or 5 x 105 TET21N cells were transfected on a 6-well plate using 7.5 µl Lipofectamine RNAiMAX (Thermo Fisher Scientific) and 50 pmol siRNA according to the manufactureŕs instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the multititre plate was pre-incubated at 30 °C for 5 minutes in a BioMate™ 3S spectrophotometer (Thermo Scientific, USA), which was set up at this temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... placed into 48-well plates and incubated overnight at 37°C 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco; Grand Island NY) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Pathology 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... The PSA-NCAM+ cells were re-plated at a density of 50 × 103 cells per cm2 to the plate coated with vitronectin (VTN-N, 5 μg/mL; ThermoFisher Scientific) and laminin (rhLaminin-521 ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were pretreated for 4-5 days with 10 mM nicotinamide before being dislodged from the plate by pipetting and treated for 5 min at 37°C with TrypLE Express (Life Technologies). Organoids were mixed with concentrated lentivirus along with 8 µg/ml polybrene and 10 µM Y27632 (SigmaAldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... Five mL of the viral supernatant and 5 ml of fresh media were added to recipient AsPC1 and Panc1 cell plates with polybrene (TR1003G, Fisher Scientific) at final concentration of 4 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... were added to 384-well plates followed by the addition of 5 μL of Lipofectamine 3000 RNAiMax Transfection Reagent (Invitrogen, 13778030), at 12.5 μL/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... 3×105 cells were seeded in a 6-well plate and transfected 24h later with 5 µg pX459 plasmid DNA using Lipofectamine 3000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
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bioRxiv - Microbiology 2023Quote: ... pylori 26695 strains were grown on Trypticase Soy Agar II plates with 5% sheep blood and in Sulfite-Free Brucella Broth containing cholesterol (Gibco-BRL) (SFBB-chol ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR plates were read on a QuantStudio™ 5 Real-time PCR system (Thermo Fisher Scientific, Applied Biosystems™, MA). The reactions were done in triplicates ...
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bioRxiv - Bioengineering 2024Quote: ... RAW 264.7 cells were maintained in tissue culture flasks or multi-well plates in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were grown in DMEM/high glucose medium supplemented with fetal bovine serum (10% FBS) ...
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bioRxiv - Biochemistry 2024Quote: ... applying the required temperature gradient across the plate using the VeriFlex technology of a QuantStudio 5 RT-PCR machine (Applied Biosystems), before lysis by addition of 1% NP-40 followed by rocking at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... cell suspension was filtered through 100 µm cell strainers and washed three times with Ca2+Mg2+ free-HBSS before counting alive cells and kept in culture (37 °C, 5% CO2) for 14 days in a tissue culture treated 24-well plate (ThermoFisher Scientific) until REP stage in TIL-RCM ...
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bioRxiv - Biophysics 2024Quote: ... cells were resuspended by pipetting and diluted by transferring 5 μL of the saturated pre-cultures to a new Nunc 384 well flat-bottom plate (Thermo Fisher) loaded with 95 μL of water per well ...
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bioRxiv - Cell Biology 2024Quote: ... were grown in T25 cm2 flasks at 28 °C or flat bottom well plates at 28 °C + 5 % CO2 in filter- sterilised M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5×10^5 cells per well were cultured overnight in a 6-well plate and surface labeled with NHS-LC biotin (Thermofisher#21335) at a concentration of 0.2 mg/ml in PBS for 20 minutes on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein expression levels were detected with enhanced chemiluminesence (SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher, 34580) using LiCor and BioRad ChemiDoc XRS imagers ...
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bioRxiv - Immunology 2022Quote: ... following the reaction with the enhanced chemiluminescence substrate (SuperSignal™ West Pico PLUS Chemiluminescent Substrate, ThermoFisher Scientific). Actin ...
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bioRxiv - Microbiology 2019Quote: ... The resolved proteins on the membrane blots were detected using the Novex ECL HRP Chemiluminescent substrate (Invitrogen) or the Luminata Forte Western HRP substrate (Millipore ...
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bioRxiv - Synthetic Biology 2019Quote: ... The blot was developed for 1-2 minutes using a SuperSignal West Pico PLUS Chemiluminescent substrate (ThermoFisher) and imaged.
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bioRxiv - Developmental Biology 2019Quote: ... The signal was revealed using a chemiluminescent reagent (Pierce® ECL Plus Western Blotting Substrate, Thermo Scientific) and was detected using hyperfilm (HyperfilmTM ECL ...
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bioRxiv - Genetics 2021Quote: ... detection was carried out using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA). Imaging was done using a ChemiDoc XRS+ imaging System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Bound antibody was detected by chemiluminescence using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) and imaged by the ChemiDoc™ MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2021Quote: ... and imaged after the addition of SuperSignal™ West Pico PLUS Chemiluminescent substrate (ThermoFisher, cat. no. 34578). Antibodies used in western blots were anti-EWSR1 (clone B-1 ...
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bioRxiv - Bioengineering 2020Quote: ... The membrane was then soaked in 25 mL of SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher) for 5 min ...
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bioRxiv - Bioengineering 2020Quote: ... Bound antibodies were visualized with the enhanced chemiluminescent detection system (Pierce) on autoradiography film (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... The PVDF membrane was incubated with Horseradish peroxidase chemiluminescent substrate (Pierce ECL Western Blotting Substrate, Thermo Scientific) before detection on photographic film and developed by an X-ray film processor (Xograph Compact X4).
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bioRxiv - Biophysics 2021Quote: ... membranes were developed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific™, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed five times in TBST then proteins were detected using enhanced chemiluminescent HRP substrate (ThermoFisher). Antibody dilutions were 1:1000 for anti-mTOR ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were visualized using a mixture of SuperSignal West Pico and Femto chemiluminescent substrates (Thermo Fisher Scientific). For the loading control visualization ...
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bioRxiv - Cell Biology 2020Quote: ... After washing and reacting the membranes with Super-Signal West Pico Chemiluminescent substrate (1856135; Thermo Fisher Scientific), signals were detected by FUSION Solo S (Vilber-Lourmat) ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were detected using the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (34580, Thermo Scientific, USA), the SuperSignal™ West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed with TBST and exposed to a chemiluminescent substrate (Thermo Fisher Scientific, Cat. #34580) for 5 minutes prior to being scanned ...