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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... rat anti-Vasa (1:100, Developmental Studies Hybridoma Bank, RRID: AB_760351) and rabbit anti-GFP (1:2500, Thermo Fisher, cat# A-11122, RRID: AB_221569). Staining was detected with the following conjugated antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated for two hours at room temperature with goat anti-Rat IgG (H+L) Alexa Fluor 488 secondary antibody (ThermoFisher Scientific, diluted 1:200). Antibodies were diluted in 1×PBS with 5% BSA and incubated in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed by washing and cells stained for 1 hr at RT with Alexa Fluor-488 anti-rat secondary antibody (Thermo Scientific, A11006, 1:1000) for CD44 and Alexa Fluor-594 anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... sc-376764) and anti-PbGAPDH rabbit polyclonal serum (1:100 dilution) or anti-mouse CD3 rat monoclonal antibody (Invitrogen, 14-0032-82; 1;100 dilution) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006; 1:500 and goat anti-rabbit conjugated to Alexa 647; ThermoFisher; A-21247; 1:500). After two final rinses in PB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and scanned using the standard Affymetrix procedure (Affymetrix GeneChip® Mouse Genome 430 2.0 Array or Affymetrix GeneChip® Rat Genome 230 2.0 Array). Quality control and low level analysis of raw fluorescence intensities was performed using the affy package (v1.64.0 ...
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bioRxiv - Neuroscience 2023Quote: Sections were washed in PBS three times for 5 min each and incubated in secondary antibodies in PBS (goat or donkey anti-rabbit, anti-mouse, or anti-rat fluorescent antibodies; Invitrogen Alexa Fluor; 1:500) for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... and HA were labeled with fluorescent secondary antibodies (donkey anti-rat IgG, 1:200; donkey anti-rabbit IgG, 1:200, Thermo-Fisher A-31573; anti-streptavidin IgG, 1:200, Invitrogen Molecular Probes S-32355), mounted onto glass slides ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were washed 3 times with PBS for 5 min each and incubated with secondary antibodies anti-mouse Alexa fluor 594 and anti-rat Alexa fluor 488 (1:250, Invitrogen #A11005 and #A11006, respectively) in blocking buffer for 1h at RT in a dark humid chamber ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed twice with PBS and incubated with a goat anti-rat Alexa Fluor 488 antibody (ThermoFisher, A-11006, 1:1000 dilution), DAPI (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... transfected keratinocytes were seeded onto a 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco BRL, Gaithersburg, MD) overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were washed 3 times with PBS for 5 min each and incubated with secondary antibodies anti-mouse Alexa fluor 594 and anti-rat Alexa fluor 488 (1:250, Invitrogen #A11032 and #A11006, respectively) in blocking buffer for 1h at RT in a dark humid chamber ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Neuroscience 2023Quote: ... and IVH-PHH (Supplementary Figure 2) P7 rats underwent intraventricular injection of 20 µl of 3 kDa Red Dextran Tetramethyl rhodamine (RD/TMR) (D3307, Thermo Fisher Scientific, Waltham, MA), 1.9-nm (1102 ...
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bioRxiv - Genomics 2023Quote: ... sections were incubated for 1 hour with a cocktail of Alexa Fluor-conjugated secondary antibodies at 1:200 (Goat anti-Rabbit IgG AF488 – Thermo Fisher Scientific A32731; Goat anti-Rat IgG AF647 – Thermo Fisher Scientific A21247), followed by counterstaining with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2 h at room temperature in the appropriate combinations of Alexa 488-594-647-conjugated anti-mouse/rabbit/guinea pig or rat IgG secondary antibodies (1:500; Molecular Probes, Eugene, OR, USA). Sections were also stained with a nuclear stain DAPI (4,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were then incubated at 4°C overnight in 1.5% Normal Donkey Serum in PBS-T with rat anti-PDGFR-β antibody (Affymetrix, 14-1402, 1:500), goat anti-CD31/platelet-endothelial cell adhesion molecule-1 (PECAM-1 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were then incubated overnight at 4 °C in rabbit anti-MYOC (1:50, custom) and rat anti-GFAP (Life Technologies, 130300, 1:500) in 5% NGS (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: Rat SMCs (A7r5) were purchased from ATCC® (Manassas, USA) and maintained in DMEM supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA). Human iPSC line SKiPS-31.3 was obtained by reprogramming of human dermal fibroblast of a healthy male adult volunteer as previously described64 ...
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bioRxiv - Neuroscience 2023Quote: Transient knockdown of NaV1.7 mRNA was done using short interfering RNA (siRNA) targeting both mouse and rat channel transcripts (Cat# 4390771, ID: s134909; Thermo Fisher Scientific Ambion, Waltham, MA, USA). Permanent knockout of endogenous NaV1.7 in ND7/23 cells was achieved using CRISPR/Cas9 genome editing with plasmid pD1301-AD (pCMV-Cas9-2A-GFP ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampi from 18 days fetal rats were dissected and incubated with trypsin (0.25% for 15 min at 37°C) (Thermo Fisher Gibco; Cat Number: 15090-046) and mechanically dissociated by trituration with a Pasteur pipette ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections six times (5 min each) and then incubated them for 2 hours in donkey anti-rat-Alexa Flour 594 antiserum (Invitrogen, Product#: A21209, Lot#: 1744721) and biotinylated donkey anti-rabbit secondary antiserum (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2024Quote: ... cells were probed with Alexa Fluor 568-conjugated goat anti-rat antibody or 488-conjugated goat anti-rabbit antibody (Invitrogen-Molecular Probes, Paisley, U.K.), diluted 1:200 or 1:1000 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Molecular Biology 2023Quote: ... kit and a TURBO DNA-free Kit (Thermo Fisher Scientific), the RNA was then further purified using RNeasy kit ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-mouse and anti-rat antibody conjugated to Alexa Fluor 488 or Alexa Fluor 546 (1:100, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) were used as secondary antibodies ...
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bioRxiv - Immunology 2019Quote: ... Samples were washed thrice with blocking buffer and incubated for 2 hr with anti-rat Alexa Fluor® 488- and anti-rabbit Alexa Fluor® 594-conjugated secondary antibodies (ThermoFisher Scientific, 1:400). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in PBS and incubated for 2 hours at room temperature in a donkey anti-rat secondary antiserum (1:500; Alexa Fluor 594, Thermo Fisher Scientific, Cat.#: A-21209). Last ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with secondary antibodies (donkey anti-rat-AF488 [A212008, Invitrogen] and donkey anti-goat AF647 [A21447, Invitrogen] diluted at 1:400 in blocking buffer) for 2 days at 4°C with shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were warmed for 1 hour at room temperature before washing for 15 minutes in 1x PBS and incubating for 90 minutes at room temperature in the secondary antibody solution consisting of a mixture of anti-rabbit Alexa Fluor® (488)with anti-rat Alexa Fluor® 568 and/or anti-guinea pig Alexa Fluor® 568(Invitrogen, Thermo Fisher Scientific) diluted in 1x PBS (1:600) ...
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bioRxiv - Developmental Biology 2022Quote: ... Dual RNAscope and immunofluorescence using probes Mm-Sntn (ACD cat.# 574191) and Mm-Mia (ACD cat.# 498011) and rat anti-Ki67 antibody (Thermo Fisher Scientific, 14-5698-82) was performed as described above for mouse samples.
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bioRxiv - Evolutionary Biology 2019Quote: ... the tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen, Cat# A11006, RRID: AB_141373), at a final dilution 1:30 and washed for 12 hours in PBS in refrigerator at +4-5°C.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for IF are as follows: donkey anti-rat IgG (H+L) highly cross-adsorbed secondary antibody (Alexa Fluor 488, 1:500, Thermo Fisher Scientific, A21208; RRID: AB_2535794), and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Pathology 2023Quote: ... and rat monoclonal IgG2a anti-LGALS3 (Cederlane, cat. no. CL8942AP, 1:250) followed by Alexa 488-conjugated donkey anti-mouse (Invitrogen, cat. no. A21202, 1:400) or Alexa 488-conjugated donkey anti-rat (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... followed by binding of the primary antibody and a detection step facilitated by the incubation with Alexa Fluor 488-conjugated goat anti-rat or anti-mouse secondary antibody (Thermo Fisher Scientific, Waltham, MA, USA). Alexa Fluor 594-conjugated streptavidin was used (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Additional L3 larvae were generated by inoculating a Sprague Dawley rat with approximately 5,000 L3 in a 0.5 ml suspension of sterile PBS (Gibco™ PBS [10X], pH 7.4, Dublin, Ireland) subcutaneously into the intrascapular area using a 23-gauge needle (BD PrecisionGlide™ Single-use Needle ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies (A11007, Alexa Fluor™ 594, goat anti-rat IgG; A11073, Alexa Fluor™ 488, goat anti-guinea pig IgG; Invitrogen, Waltham, Massachusetts, USA) were diluted 1:250 in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies diluted in 3% BSA/PBST were incubated with the samples at 37°C for 1 h: goat anti-rat IgG (H+L) (Thermo Fisher Scientific A11006, 1:100), goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibodies (1:500 Goat anti -rabbit Alexa Fluor 647 [Thermo Fisher Scientific, A-21245], 1:500 Donkey anti-rat Alexa Fluor 594 [Thermo Fisher Scientific, A-21209]) in PBS for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibodies (1:500 Goat anti -rabbit Alexa Fluor 647 [Thermo Fisher Scientific, A-21245], 1:500 Donkey anti-rat Alexa Fluor 594 [Thermo Fisher Scientific, A-21209]) in PBS for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampi from 18 days fetal rats were dissected and incubated with trypsin (0.25% for 15 min at 37°C) (Thermo Fisher Gibco; Cat Number: 15090-046) and mechanically dissociated by trituration with a Pasteur pipette ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were washed in PBS-T 3×5min and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA, USA) for 2 hours at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then washed three times followed by an incubation for 1 h with the following secondary antibodies: goat anti-rat Alexa Fluor 647 antibody (Thermo Fisher, #A78947, 1 µg/ml), goat anti-mouse Alexa Flour 488 antibody (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in 500 μL of primary antibody solution containing rat anti-alpha-tubulin YL1/2 (MA1-80017, Invitrogen, CA, SAD, diluted 1:500) overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-rabbit secondary antibody conjugated with green Alexa Flour 488 and donkey anti-rat secondary antibody conjugated with green Alexa Flour 488 were purchased from Invitrogen (cat. # A21206 and cat. # A21208). Confocal microscopic images were acquired using a Zeiss 710 laser scanning confocal microscope and the associated image acquisition and processing software Zen.
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bioRxiv - Neuroscience 2024Quote: ... the dissected tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen, Cat# A11006, RRID: AB_141373) at a final dilution 1:40.
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bioRxiv - Cancer Biology 2022Quote: ... Kit (ThermoFisher Scientific) and subsequently 10 clones per cell line were sequenced (LightRun GATC Carlo Erba) ...
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bioRxiv - Genetics 2020Quote: ... coli kit (Invitrogen). The transfection was performed according to the manufacturer’s guidelines and vector assembly was tested by Sanger sequencing.
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bioRxiv - Systems Biology 2022Quote: A kit (Ambion Magmax™-96 total RNA isolation kit ...